Microfluidics-based rapid measurement of nitrite in human blood plasma

Gaikwad, R; Thangaraj, P R; Sen, A. K.

doi:10.1039/d2an00020b

Cited by 2 publications

(3 citation statements)

References 69 publications

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“…Principally, our sensor showed impressive LoD and sensitivity. From previous studies, the nitrite concentration in healthy human blood is in the range of 0.4–1.2 µM [ 60 ]. The wide linear range of this nitrite sensor can be useful to analyze nitrite levels in real samples directly, without diluting samples.…”

Section: Resultsmentioning

confidence: 99%

An Electroanalytical Enzymeless α-Fe2O3-ZnO Hybrid Nanostructure-Based Sensor for Sensitive Quantification of Nitrite Ions

Ahmad,

Abdullah,

Rehman

et al. 2024

Nanomaterials

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Nitrite monitoring serves as a fundamental practice for protecting public health, preserving environmental quality, ensuring food safety, maintaining industrial safety standards, and optimizing agricultural practices. Although many nitrite sensing methods have been recently developed, the quantification of nitrite remains challenging due to sensitivity and selectivity limitations. In this context, we present the fabrication of enzymeless iron oxide nanoparticle-modified zinc oxide nanorod (α-Fe2O3-ZnO NR) hybrid nanostructure-based nitrite sensor fabrication. The α-Fe2O3-ZnO NR hybrid nanostructure was synthesized using a two-step hydrothermal method and characterized in detail utilizing x-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). These analyses confirm the successful synthesis of an α-Fe2O3-ZnO NR hybrid nanostructure, highlighting its morphology, purity, crystallinity, and elemental constituents. The α-Fe2O3-ZnO NR hybrid nanostructure was used to modify the SPCE (screen-printed carbon electrode) for enzymeless nitrite sensor fabrication. The voltammetric methods (i.e., cyclic voltammetry (CV) and differential pulse voltammetry (DPV)) were employed to explore the electrochemical characteristics of α-Fe2O3-ZnO NR/SPCE sensors for nitrite. Upon examination of the sensor’s electrochemical behavior across a range of nitrite concentrations (0 to 500 µM), it is evident that the α-Fe2O3-ZnO NR hybrid nanostructure shows an increased response with increasing nitrite concentration. The sensor demonstrates a linear response to nitrite concentrations up to 400 µM, a remarkable sensitivity of 18.10 µA µM−1 cm−2, and a notably low detection threshold of 0.16 µM. Furthermore, its exceptional selectivity, stability, and reproducibility make it an ideal tool for accurately measuring nitrite levels in serum, yielding reliable outcomes. This advancement heralds a significant step forward in the field of environmental monitoring, offering a potent solution for the precise assessment of nitrite pollution.

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Section: Resultsmentioning

confidence: 99%

An Electroanalytical Enzymeless α-Fe2O3-ZnO Hybrid Nanostructure-Based Sensor for Sensitive Quantification of Nitrite Ions

Ahmad,

Abdullah,

Rehman

et al. 2024

Nanomaterials

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“…Therefore, identification of a method characterized by instant monitoring, low cost, and easy operation has become a research hotspot. Paper-based analytical devices (PADs) may provide point-of-care diagnostics with a potential solution [68][69][70][71][72][73][74][75][76][77][78][79], as summarized in Table 5. The schematic assembly of the PAD reported by Noiphung et al [70] is shown in Figure 11.…”

Section: Paper-based Analytical Devicesmentioning

confidence: 99%

“…Gaikwad et al [78] reported a fluorescence-based microfluidic method to improve the sensitivity of µPADs. In the range of 0 µM−20 µM, there was good linearity, and sensitivity was greatly improved (LOD: 60 nM).…”

Section: Paper-based Analytical Devicesmentioning

confidence: 99%

Advancements in Preprocessing and Analysis of Nitrite and Nitrate since 2010 in Biological Samples: A Review

Liu,

Guo,

Zhao

et al. 2023

Molecules

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As a substance present in organisms, nitrite is a metabolite of nitric oxide and can also be ingested. Nitrate is the metabolite of nitrite. Therefore, it is necessary to measure it quickly, easily and accurately to evaluate the health status of humans. Although there have been several reviews on analytical methods for non-biological samples, there have been no reviews focused on both sample preparation and analytical methods for biological samples. First, rapid and accurate nitrite measurement has significant effects on human health. Second, the detection of nitrite in biological samples is problematic due to its very low concentration and matrix interferences. Therefore, the pretreatment plus measuring methods for nitrite and nitrate obtained from biological samples since 2010 are summarized in the present review, and their prospects for the future are proposed. The treatment methods include liquid–liquid microextraction, various derivatization reactions, liquid–liquid extraction, protein precipitation, solid phase extraction, and cloud point extraction. Analytical methods include spectroscopic methods, paper-based analytical devices, ion chromatography, liquid chromatography, gas chromatography–mass spectrometry, electrochemical methods, liquid chromatography–mass spectrometry and capillary electrophoresis. Derivatization reagents with rapid quantitative reactions and advanced extraction methods with high enrichment efficiency are also included. Nitrate and nitrate should be determined at the same time by the same analytical method. In addition, much exploration has been performed on formulating fast testing through microfluidic technology. In this review, the newest developments in nitrite and nitrate processing are a focus in addition to novel techniques employed in such analyses.

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Microfluidics-based rapid measurement of nitrite in human blood plasma

Abstract: Nitric oxide (NO) is one of the vital gasotransmitters that takes part in many biological pathways such as infection, inflammation and ischemia, immune response, neurotransmission, and cardiovascular systems. Nitrite is...

Cited by 2 publications

References 69 publications

An Electroanalytical Enzymeless α-Fe2O3-ZnO Hybrid Nanostructure-Based Sensor for Sensitive Quantification of Nitrite Ions

An Electroanalytical Enzymeless α-Fe2O3-ZnO Hybrid Nanostructure-Based Sensor for Sensitive Quantification of Nitrite Ions

Advancements in Preprocessing and Analysis of Nitrite and Nitrate since 2010 in Biological Samples: A Review

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