Microfluidics Based Magnetophoresis: A Review

Alnaimat, Fadi; Dagher, Sawsan; Mathew, Bobby; Hilal-Alnqbi, Ali; Khashan, Saud

doi:10.1002/tcr.201800018

Cited by 117 publications

(83 citation statements)

References 114 publications

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“…On the other hand, when dealing with magnetic beads as capture probes, the strong particle-particle interactions often create stable aggregates which hinders their further utilization. In addition, the entrapment of nonspecific impurities of sample in the capturing regions might affect the performances of the device, in terms of recovery efficiency and purity, making this approach suitable only for certain analytical applications [ 175 ].…”

Section: Isolation Of Exosomes and Microvesicles From Biological Fmentioning

confidence: 99%

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Chiriaco

Bianco

Nigro

et al. 2018

Sensors

125

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Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.

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Section: Isolation Of Exosomes and Microvesicles From Biological Fmentioning

confidence: 99%

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Chiriaco

Bianco

Nigro

et al. 2018

Sensors

125

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show abstract

“…So the crucial factors are the magnitude and non-uniformity of the magnetic field, particle/cell magnetic susceptibilities, and suspension media. Because of its advantages of high penetrability, low-heat generation, being gentle, and non-destructive for proteins or peptides, magnetophoresis is greatly utilized to manipulate cells, such as cell separation, trapping, and measurements, when combined with microfluidic technology [93,94]. Zhao et al made a biocompatible ferrofluid as the media to separate a variety of low-concentration (~100 cancer cells/mL) cancer cells with a high throughput of 1.2 mL/h from undiluted white blood cells.…”

Section: Biomedical Sorting and Diagnosticsmentioning

confidence: 99%

The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review

Song

et al. 2020

Micromachines

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Microfluidic systems have been widely explored based on microfluidic technology, and it has been widely used for biomedical screening. The key parts are the fabrication of the base scaffold, the construction of the matrix environment in the 3D system, and the application mechanism. In recent years, a variety of new materials have emerged, meanwhile, some new technologies have been developed. In this review, we highlight the properties of high throughput and the biomedical application of the microfluidic chip and focus on the recent progress of the fabrication and application mechanism. The emergence of various biocompatible materials has provided more available raw materials for microfluidic chips. The material is not confined to polydimethylsiloxane (PDMS) and the extracellular microenvironment is not limited by a natural matrix. The mechanism is also developed in diverse ways, including its special physical structure and external field effects, such as dielectrophoresis, magnetophoresis, and acoustophoresis. Furthermore, the cell/organ-based microfluidic system provides a new platform for drug screening due to imitating the anatomic and physiologic properties in vivo. Although microfluidic technology is currently mostly in the laboratory stage, it has great potential for commercial applications in the future.

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“…Among them, magnetic beads are employed via specific or non-specific adsorption of surface-modified magnetic beads with the corresponding targeted DNA to form new complexes, which are then separated from other non-targeted substances under the influence of magnetic field. Based on the methods in which the magnetic field is created, magnetophoretic separation can be divided into three categories: electromagnet separation [8,9], integrated soft magnet separation [10][11][12], and external permanent magnet separation [13][14][15]. In terms of electromagnet separation, it produces a small magnetic force but flexibly controls the magnitude of the magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Continuous Microfluidic Purification of DNA Using Magnetophoresis

et al. 2020

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Automatic microfluidic purification of nucleic acid is predictable to reduce the input of original samples and improve the throughput of library preparation for sequencing. Here, we propose a novel microfluidic system using an external NdFeB magnet to isolate DNA from the polymerase chain reaction (PCR) mixture. The DNA was purified and isolated when the DNA-carrying beads transported to the interface of multi-laminar flow under the influence of magnetic field. Prior to the DNA recovery experiments, COMSOL simulations were carried out to study the relationship between trajectory of beads and magnet positions as well as fluid velocities. Afterwards, the experiments to study the influence of varying velocities and input of samples on the DNA recovery were conducted. Compared to experimental results, the relative error of the final position of beads is less than 10%. The recovery efficiency decreases with increase of input or fluid velocity, and the maximum DNA recovery efficiency is 98.4% with input of l00 ng DNA at fluid velocity of 1.373 mm/s. The results show that simulations significantly reduce the time for parameter adjustment in experiments. In addition, this platform uses a basic two-layer chip to realize automatic DNA isolation without any other liquid switch value or magnet controller.

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Microfluidics Based Magnetophoresis: A Review

Cited by 117 publications

References 114 publications

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review

Continuous Microfluidic Purification of DNA Using Magnetophoresis

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