Microfluidics Based Generation of Curcumin Loaded Microfibrous Mat against Staphylococcus aureus Biofilm by Photodynamic Therapy

Abstract

The rapid increase in multidrug resistant biofilm infections is a major concern for global health. A highly effective therapy is required for the treatment of biofilm related infections. In this study, curcumin loaded alginate microfibers were generated by using the microfluidic technique. In this strategy, alginate microfibers are used as a carrier for the encapsulation of curcumin and then are irradiated with blue light to assess the efficacy of a combined therapy (blue light + curcumin) against drug resistant Staphylococcus aureus (S. aureus). The advantage of utilizing photodynamic therapy (PDT) is the usage of a non-antibiotic mode to inactivate bacterial cells. In the presence of blue light, the curcumin loaded alginate microfibers have shown good eradication activity against biofilms formed by multidrug resistant S. aureus. We achieved different diameters of curcumin loaded alginate microfibers through manipulation of flow rates. The curcumin loaded microfibers were characterized for their size, morphology, and curcumin encapsulation. Further, the efficacy of these microfibers in the presence of blue light has been evaluated against biofilm forming S. aureus (NCIM 5718) through optical and electron microscopy. This study employs microfluidic techniques to obtain an efficacious and cost-effective microfibrous scaffold for controlled release of curcumin to treat biofilms in the presence of blue light.

A biomass-derived dual crosslinked DNA-nanoparticle hydrogel for visible light-induced photodynamic bacterial inactivation

“…5A and B). DNA-NP was found to inhibit the growth of biofilm significantly up to ∼76% for E. coli and ∼82% for S. aureus , respectively, as quantified through the OD taken at 595 nm 30 (Fig. 5C).…”

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

“…Recently, Sharma et al used alginate microfibers as a carrier of curcumin, followed by being irradiated with blue light (450 nm) to assess the efficacy of the combined therapy against MRSA. The curcumin-loaded microfibers exhibited great potential when combined with photodynamic therapy, which resulted in the complete eradication of the MRSA biofilms [ 140 ]. These results suggested that the combination of natural products and photodynamic therapy is a potent tool against biofilm-forming bacteria that can reduce their antimicrobial resistance.…”

Quaternized Silk Fibroin Nanocarriers Loaded with Chlorin e6 for Photodynamic Elimination of Drug-Resistant Bacteria and Biofilms

“…At present, multidrug-resistant (MDR) bacterial infections remain a serious public health threat and may have lasting effects on the normal functioning of affected organs. − The common clinical approach to eliminating bacterial infections relies heavily on antibiotic therapy. − However, the therapeutic efficacy is inevitably undermined, and the bacterial resistance has increased with the irrational and indiscriminate use of antibiotics. , In addition, most pathogenic bacteria do not typically exist in the human environment as individual organisms or in planktonic states. Instead, they tend to form populations known as biofilms, making it difficult for small-molecule antimicrobial agents to penetrate effectively and ultimately leading to insensitivity to antimicrobial agents. − Therefore, the development of a new antimicrobial nanomaterial targeting biofilms formed by drug-resistant bacteria is urgently needed.…”