Microfluidics-based device for the measurement of blood viscosity and its modeling based on shear rate, temperature, and heparin concentration

Khnouf, Ruba; Karasneh, Dina; Abdulhay, Enas; Abdelhay, Arwa; Sheng, Weian; Fan, Z. Hugh

doi:10.1007/s10544-019-0426-5

Cited by 27 publications

(26 citation statements)

References 33 publications

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“…A microfluidic device has several advantages, including small volume consumption, fast measurement, easy sample handling, high sensitivity, and disposability. Thus, it has been widely used to measure various biophysical properties of blood samples (i.e., blood viscosity [15], RBCs aggregation [16], RBCs deformability [17,18], and hematocrit [19]).…”

Section: Introductionmentioning

confidence: 99%

“…Second, various devices such as a microelectromechanical system (MEMS)-based microfluidic device, a 3D-printed microfluidic device [13,24], and a paper-based device [25] have been suggested for inducing blood flow in a specifically constrained direction. Third, quantification techniques such as advancing meniscus (i.e., variations of a blood column over time) [15,22,26,27], the falling time of a metal sphere in a tube [28], electric impedances (i.e., resistance, capacitance) [29,30], droplet length [31], digital flow compartment with a microfluidic channel array [11,12], interface detection in co-flowing streams [32,33], and reversal flow switching in a Wheatstone bridge analog of a fluidic circuit [14] have been suggested to measure blood viscosity.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic-Based Biosensor for Blood Viscosity and Erythrocyte Sedimentation Rate Using Disposable Fluid Delivery System

Kang

2020

Micromachines

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To quantify the variation of red blood cells (RBCs) or plasma proteins in blood samples effectively, it is necessary to measure blood viscosity and erythrocyte sedimentation rate (ESR) simultaneously. Conventional microfluidic measurement methods require two syringe pumps to control flow rates of both fluids. In this study, instead of two syringe pumps, two air-compressed syringes (ACSs) are newly adopted for delivering blood samples and reference fluid into a T-shaped microfluidic channel. Under fluid delivery with two ACS, the flow rate of each fluid is not specified over time. To obtain velocity fields of reference fluid consistently, RBCs suspended in 40% glycerin solution (hematocrit = 7%) as the reference fluid is newly selected for avoiding RBCs sedimentation in ACS. A calibration curve is obtained by evaluating the relationship between averaged velocity obtained with micro-particle image velocimetry (μPIV) and flow rate of a syringe pump with respect to blood samples and reference fluid. By installing the ACSs horizontally, ESR is obtained by monitoring the image intensity of the blood sample. The averaged velocities of the blood sample and reference fluid (<UB>, <UR>) and the interfacial location in both fluids (αB) are obtained with μPIV and digital image processing, respectively. Blood viscosity is then measured by using a parallel co-flowing method with a correction factor. The ESR is quantified as two indices (tESR, IESR) from image intensity of blood sample (<IB>) over time. As a demonstration, the proposed method is employed to quantify contributions of hematocrit (Hct = 30%, 40%, and 50%), base solution (1× phosphate-buffered saline [PBS], plasma, and dextran solution), and hardened RBCs to blood viscosity and ESR, respectively. Experimental Results of the present method were comparable with those of the previous method. In conclusion, the proposed method has the ability to measure blood viscosity and ESR consistently, under fluid delivery of two ACSs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic-Based Biosensor for Blood Viscosity and Erythrocyte Sedimentation Rate Using Disposable Fluid Delivery System

Kang

2020

Micromachines

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“…In addition to temperature, hemodiluents and the fluid’s flow rate variation also impacts blood behavior [ 9 ]. Blood has a non-Newtonian (viscosity shear dependents) behavior at shear rates below 150 s −1 [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Temperature and Flow Rate on the Cell-Free Area in the Microfluidic Channel

2021

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Blood cell manipulation in microdevices is an interesting task for the separation of particles, by their size, density, or to remove them from the buffer, in which they are suspended, for further analysis, and more. This study highlights the cell-free area (CFA) widening based on experimental results of red blood cell (RBC) flow, suspended in a microfluidic device, while temperature and flow rate incrementally modify RBC response within the microflow. Studies of human red blood cell flow, at a concentration of 20%, suspended in its autologous plasma and phosphate-buffered saline (PBS) buffer, were carried out at a wide flow rate, varying between 10 and 230 μL/min and a temperature range of 23 °C to 50 °C. The plotted measures show an increment in a CFA near the channel wall due to cell flow inertia after a constricted channel, which becomes more significant as temperature and flow rate increase. The temperature increment widened the CFA up to three times. In comparison, flow rate increment increased the CFA up to 20 times in PBS and 11 times in plasma.

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“…Syringe pumps are an excellent tool used in many different fields: from physics [1], flow chemistry [2], microfluidics [3,4], to biology and microscopy [5]. However, even a simple syringe pump, with little possibility of automation, can cost close to €1000 [6].…”

Section: Introductionmentioning

confidence: 99%

Ender3 3D Printer Kit Transformed into Open, Programmable Syringe Pump Set

Baas¹,

Saggiomo

2021

Preprint

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Microfluidics-based device for the measurement of blood viscosity and its modeling based on shear rate, temperature, and heparin concentration

Cited by 27 publications

References 33 publications

Microfluidic-Based Biosensor for Blood Viscosity and Erythrocyte Sedimentation Rate Using Disposable Fluid Delivery System

Microfluidic-Based Biosensor for Blood Viscosity and Erythrocyte Sedimentation Rate Using Disposable Fluid Delivery System

Effect of Temperature and Flow Rate on the Cell-Free Area in the Microfluidic Channel

Ender3 3D Printer Kit Transformed into Open, Programmable Syringe Pump Set

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