Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip

Godin, Jessica; Chen, Chun‐Hao; Cho, Sung Hwan; Qiao, Wen; Tsai, Frank S.; Lo, Yu‐Hwa

doi:10.1002/jbio.200810018

Cited by 149 publications

(107 citation statements)

References 96 publications

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“…9,12,13 Thus, this review article will focus on recent advances in developing a miniaturized, microfluidic flow cytometer, where optics and microfluidics are highly integrated on a tiny chip for novel functionalities.…”

Section: A Flow Cytometry-principles and Applications In Biomedical mentioning

confidence: 99%

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Review Article: Recent advancements in optofluidic flow cytometer

Cho¹,

Godin²,

Chen³

et al. 2010

Biomicrofluidics

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139

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There is an increasing need to develop optofluidic flow cytometers. Optofluidics, where optics and microfluidics work together to create novel functionalities on a small chip, holds great promise for lab-on-a-chip flow cytometry. The development of a low-cost, compact, handheld flow cytometer and microfluorescence-activated cell sorter system could have a significant impact on the field of point-of-care diagnostics, improving health care in, for example, underserved areas of Africa and Asia, that struggle with epidemics such as HIV/AIDS. In this paper, we review recent advancements in microfluidics, on-chip optics, novel detection architectures, and integrated sorting mechanisms.

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Section: A Flow Cytometry-principles and Applications In Biomedical mentioning

confidence: 99%

“…For other nonfluorescence-activated cell sorting mechanisms, such as magnetophoresis 77 and acoustophoresis, 78 readers can refer to a number of recently published articles. 12,[79][80][81] …”

Section: Microfluidic Fluorescence-activated Cell Sorter " Facs…mentioning

confidence: 99%

Review Article: Recent advancements in optofluidic flow cytometer

Cho¹,

Godin²,

Chen³

et al. 2010

Biomicrofluidics

Self Cite

139

105

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“…Indeed, the microfluidic approach favors analysis at the single-cell level, a reduced sample contamination, an increased analysis sensitivity and a higher processing automation 1,2 . To be truly competitive with standard instrumentation, these microfluidic devices should satisfy several challenging constraints: (i) a high throughput, necessary to process large amounts of samples in a reasonable amount of time; (ii) a low pressure drop over the microfluidic device, which basically means low fluidic resistance, thus allowing vacuum driven flows; (iii) optical accessibility inside the microchannel to allow high quality optical detection 3 .…”

Section: Introductionmentioning

confidence: 99%

Particle focusing by 3D inertial microfluidics

et al. 2017

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Three-dimensional (3D) particle focusing in microfluidics is a fundamental capability with a wide range of applications, such as on-chip flow cytometry, where high-throughput analysis at the single-cell level is performed. Currently, 3D focusing is achieved mainly in devices with complex layouts, additional sheath fluids, and complex pumping systems. In this work, we present a compact microfluidic device capable of 3D particle focusing at high flow rates and with a small footprint, without the requirement of external fields or lateral sheath flows, but using only a single-inlet, single-outlet microfluidic sequence of straight channels and tightly curving vertical loops. This device exploits inertial fluidic effects that occur in a laminar regime at sufficiently high flow rates, manipulating the particle positions by the combination of inertial lift forces and Dean drag forces. The device is fabricated by femtosecond laser irradiation followed by chemical etching, which is a simple two-step process enabling the creation of 3D microfluidic networks in fused silica glass substrates. The use of tightly curving three-dimensional microfluidic loops produces strong Dean drag forces along the whole loop but also induces an asymmetric Dean flow decay in the subsequent straight channel, thus producing rapid cross-sectional mixing flows that assist with 3D particle focusing. The use of out-of-plane loops favors a compact parallelization of multiple focusing channels, allowing one to process large amounts of samples. In addition, the low fluidic resistance of the channel network is compatible with vacuum driven flows. The resulting device is quite interesting for high-throughput on-chip flow cytometry.

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“…As a result, clinical flow cytometry assays have been relegated to well-equipped, centralized laboratories. [34][35][36][37][38][39] In order to overcome the limitations of conventional flow cytometry systems, researchers have made significant efforts to developing microfluidics-based miniature flow cytometry devices that could be more accessible and affordable for research laboratories and clinics. 10,12,[40][41][42][43][44][45][46][47][48][49][50][51][52][53] In these approaches, the key is to develop microfluidic structures to focus particles/cells three-dimensionally.…”

Section: Introductionmentioning

confidence: 99%

An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing

et al. 2012

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In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate onchip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a singlelayer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics. V C 2012 American Institute of Physics. [http://dx

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Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip

Cited by 149 publications

References 96 publications

Review Article: Recent advancements in optofluidic flow cytometer

Review Article: Recent advancements in optofluidic flow cytometer

Particle focusing by 3D inertial microfluidics

An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing

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