Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA

Ölçer, Zehra; Esen, Elif; Ersoy, Aylin; Budak, Sinan; Kaya, Dılşa Mızrak; Gok, M.; Barut, Serkan; Üstek, Duran; Uludag, Yildiz

doi:10.1016/j.bios.2015.03.052

Cited by 39 publications

(13 citation statements)

References 38 publications

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“…Several reviews have been published over recent years about the plenitude of microfluidics application [153,154,155,156,157], although, in light of this review, the development of microfluidics chips to detect microorganisms are the most relevant [152,158,159,160]. Detection of pathogenic bacteria and viruses using Chips is possible by recurring small sample volumes with great sensitivity, and it has huge applicability in food safety control, environmental monitoring, and clinical diagnosis.…”

Section: Biochemical Analytical Methods To Detect Microorganismsmentioning

confidence: 99%

“…Recent approaches for microbial detection and identification are focusing on combinations of analytical standard techniques within microfluidics chips, without the need for labeling procedures. In particular, there are several reports of combinations of microfluidics devices with PCR [161,162], MS approaches [163,164,165], spectrometry [166], electrochemistry [154,167], among others.…”

Section: Biochemical Analytical Methods To Detect Microorganismsmentioning

confidence: 99%

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Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

et al. 2019

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Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.

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Section: Biochemical Analytical Methods To Detect Microorganismsmentioning

confidence: 99%

Section: Biochemical Analytical Methods To Detect Microorganismsmentioning

confidence: 99%

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

et al. 2019

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“…An electroactive substrate is then added to the chip for electrochemical readout. The LOD of 6 pM was obtained with an assay that takes about 75 min to complete [102]. …”

Section: General Overview Of Applicationsmentioning

confidence: 99%

Gold Nanoparticles for Diagnostics: Advances towards Points of Care

et al. 2016

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted developments in the exploration of biomolecular interactions with AuNP-containing systems, in particular for biomedical applications in diagnostics. These systems show great promise in improving sensitivity, ease of operation and portability. Despite this endeavor, most platforms have yet to reach maturity and make their way into clinics or points of care (POC). Here, we present an overview of emerging and available molecular diagnostics using AuNPs for biomedical sensing that are currently being translated to the clinical setting.

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“…In this work, a new automated biosensor device (MiSens) has been used, where the assay has been performed using a microfluidic channel integrated biochip with the sequential injections of the assay reagents. [28][29][30][31] MiSens biosensor device relies on the Real-time Electrochemical Profiling (REP™) technology and has been utilized to investigate the reversible binding of glycoprotein peroxidase on the phenylboronic acid coated biochip surface. Aminophenylboronic acid modified self-assembled monolayer (SAM), immobilization of horseradish peroxidase, and potentiometric measurements, cyclic voltammetry (CV), chronoamperometry, have been carried out in the biosensor device.…”

Section: Introductionmentioning

confidence: 99%

Design of an Automated Electrochemical Biosensor Modified with Phenylboronic Acid to Study Glycoprotein Immobilization

Ölçer

2020

ChemistrySelect

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It is important that biosensor surface chemistry assays because of the easy immobilization of biological sensing molecules on the chip surface and especially not damage of these molecules through surface effects. Boronic acid modified self-assembled monolayers can be formed on gold electrodes and used immobilizations of glycoproteins. Glycoproteins which have short oligosaccharides exist in various cellular events with different functions and serve in many cellular events as cell surface recognition. It was successfully fabricated a new phenylboronic acid self-assembled layer on gold electrodes for immobilization of glycoproteins, where 4-aminophenylboronic acid (APBA) is covalently bound to the 11-mercapto-1undecanol (MUOL) layer with glutaraldehyde linkage. The specific binding of glycoprotein with self-assebled layer was studied using horseradish peroxidase (HRP) as a model protein. All the experiments, voltametric and amperometric measurements were carried out during microfluidic flow first time using automated new biosensor device that enabled fast and easy modification of electrode surface with boronic acid and immobilization of the HRP protein in a short period of time.

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Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA

Cited by 39 publications

References 38 publications

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

Gold Nanoparticles for Diagnostics: Advances towards Points of Care

Design of an Automated Electrochemical Biosensor Modified with Phenylboronic Acid to Study Glycoprotein Immobilization

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