Microfluidics analysis of red blood cell membrane viscoelasticity

Tomaiuolo, Giovanna; Barra, Mario; Preziosi, Valentina; Cassinese, A.; Rotoli, Bruno; Guido, Stefano

doi:10.1039/c0lc00348d

Cited by 119 publications

(96 citation statements)

References 32 publications

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“…Some examples are the measurement of the RBC cellular trajectories and deformation under a transient high shear stress in microchannels with a rectangular cross-section 20,21 and the determination of cell deformability of a single RBC flowing in a microfluidic device with a microchannel thickness comparable to the RBC size. 19 Studies focusing on the effect of extensional flow field on RBC flow and deformability are scarce despite extensional flow often being present in the microcirculation, particularly when there is a sudden change in geometry, e.g., in stenosis and in microvascular networks composed of short irregular vessel segments linked by numerous bifurcations. In converging flows such as those found in microstenoses, extensional flow can be generated due to the dramatic change of velocity when the fluid flows from a wide to a narrow region of the channel.…”

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confidence: 99%

“…The distinct advantage of the microfluidic devices to test a large number of cells using only a small amount of blood has prompted a vast amount of research in this field. 3,8,12,15,[19][20][21] However, most of the proposed devices focus mainly on the effect of shear flow alone. Some examples are the measurement of the RBC cellular trajectories and deformation under a transient high shear stress in microchannels with a rectangular cross-section 20,21 and the determination of cell deformability of a single RBC flowing in a microfluidic device with a microchannel thickness comparable to the RBC size.…”

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Human red blood cell behavior under homogeneous extensional flow in a hyperbolic-shaped microchannel

et al. 2013

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It is well known that certain pathological conditions result in a decrease of red blood cells (RBCs) deformability and subsequently can significantly alter the blood flow in microcirculation, which may block capillaries and cause ischemia in the tissues. Microfluidic systems able to obtain reliable quantitative measurements of RBC deformability hold the key to understand and diagnose RBC related diseases. In this work, a microfluidic system composed of a microchannel with a hyperbolic-shaped contraction followed by a sudden expansion is presented. We provide a detailed quantitative description of the degree of deformation of human RBCs under a controlled homogeneous extensional flow field. We measured the deformation index (DI) as well as the velocity of the RBCs travelling along the centerline of the channel for four different flow rates and analyze the impact of the particle Reynolds number. The results show that human RBC deformation tends to reach a plateau value in the region of constant extensional rate, the value of which depends on the extension rate. Additionally, we observe that the presence of a sudden expansion downstream of the hyperbolic contraction modifies the spatial distribution of cells and substantially increases the cell free layer (CFL) downstream of the expansion plane similarly to what is seen in other expansion flows. Beyond a certain value of flow rate, there is only a weak effect of inlet flow rates on the enhancement of the downstream CFL. These in vitro experiments show the potential of using microfluidic systems with hyperbolic-shaped microchannels both for the separation of the RBCs from plasma and to assess changes in RBC deformability in physiological and pathological situations for clinical purposes. However, the selection of the geometry and the identification of the most suitable region to evaluate the changes on the RBC deformability under extensional flows are crucial if microfluidics is to be used as an in vitro clinical methodology to detect circulatory diseases. V C 2013 AIP Publishing LLC. [http://dx

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Human red blood cell behavior under homogeneous extensional flow in a hyperbolic-shaped microchannel

et al. 2013

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“…Red blood cells (RBCs) play an important role in cell metabolism and aerobic respiration [1,2]. The physical and mechanical features of RBCs have been regarded as an important indicator in the studies of bio-processes, pathology, and toxicology [3,4].…”

Section: Introductionmentioning

confidence: 99%

Rotation and deformation of human red blood cells with light from tapered fiber probes

Liu

Huang

et al. 2016

Nanophotonics

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Dynamic rotation and deformation of human red blood cells (RBCs) are extremely important to investigate the survival and mechanical features of cells, which will be of great physiological and pathological significance. Here, we report an optical approach that is capable of both rotating and deforming RBCs with light from two tapered fiber probes (TFPs). With laser beams at the wavelength of 980 nm injected into the TFPs, a single RBC was rotated around different axes while single or multiple RBCs were stretched by adjusting the points of action and magnitude of the optical forces from the TFPs. The biological safety of the approach was also discussed by taking the laser power required into account.

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“…Abkarian et al [23][24] experimentally observed the alterations of deformability between healthy and unhealthy RBC in a microchannel. Later, the deformation of RBC subjected to pressure-driven flows in a microchannel has been comprehensively studied [25][26][27]. Recently, Chen et al [28] fabricated a lab-on-a-chip device with a capillary network to study the RBC hydrodynamics.…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic motion of a deformable particle: Dielectrophoretic effect

Mauroy

Sharma

et al. 2011

Electrophoresis

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Electrokinetics-induced motion and deformation of a hyperelastic particle confined in a slit microchannel has been numerically investigated for the first time with a full consideration of the fluid-particle-electric field interactions and the dielectrophoretic (DEP) effect. When the initial orientation of a cylindrical particle with respect to the applied electric field, θ p0 , is 90 o , the particle tends to curl up as a "C" shape when moving from left to right. The electrokineticsinduced particle deformation is due to the joint effects of the shear force arising from the nonuniform Smoluchowski slip velocity on the particle surface, and the asymmetric DEP force with respect to the center of the deformed particle arising from the spatially non-uniform electric field surrounding the particle. The electrokinetics-induced particle deformation is opposite to that of a particle moving in the same direction subjected to a pressure-driven flow. When the initial particle orientation is 0 < θ p0 < 90 o , a net torque arising from the DEP effect progressively rotates and aligns the particle with its longest axis parallel to the applied electric field, thus decreasing the non-uniformity of the electric field and accordingly the particle deformation. The numerical predictions are in qualitative agreement with our previous experimental observation. The results show that the DEP effect is significant and must be taken into account in the modeling of electrokinetic motion of a deformable particle in microfluidics.3

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Microfluidics analysis of red blood cell membrane viscoelasticity

Cited by 119 publications

References 32 publications

Human red blood cell behavior under homogeneous extensional flow in a hyperbolic-shaped microchannel

Human red blood cell behavior under homogeneous extensional flow in a hyperbolic-shaped microchannel

Rotation and deformation of human red blood cells with light from tapered fiber probes

Electrokinetic motion of a deformable particle: Dielectrophoretic effect

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