Microfluidic Western Blot

Pan, Wenying; Chen, Wei; Jiang, Xingyu

doi:10.1021/ac1000493

Cited by 70 publications

(67 citation statements)

References 29 publications

(43 reference statements)

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“…Moreover, such assays require retrieval of the biological material from the microdevice, a feature that is often not considered in designing organs-on-a-chip. The development and integration of highly sensitive microfluidic PCR, 60 western blot assays, 27,61,62 and flow cytometry 104 systems into microdevices may alleviate these constraints, but much study remains to be done before these microfluidic modules can be considered as readily available and accessible to researchers engaged in this field. Hence, the practical utility of such microengineered platforms can often be limited in obtaining biological information, at sufficient throughput for robust hypothesis testing.…”

Section: Discussionmentioning

confidence: 99%

Organs-on-a-Chip: A Focus on Compartmentalized Microdevices

et al. 2011

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Advances in microengineering technologies have enabled a variety of insights into biomedical sciences that would not have been possible with conventional techniques. Engineering microenvironments that simulate in vivo organ systems may provide critical insight into the cellular basis for pathophysiologies, development, and homeostasis in various organs, while curtailing the high experimental costs and complexities associated with in vivo studies. In this article, we aim to survey recent attempts to extend tissue-engineered platforms toward simulating organ structure and function, and discuss the various approaches and technologies utilized in these systems. We specifically focus on microtechnologies that exploit phenomena associated with compartmentalization to create model culture systems that better represent the in vivo organ microenvironment.

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Section: Discussionmentioning

confidence: 99%

Organs-on-a-Chip: A Focus on Compartmentalized Microdevices

et al. 2011

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“…We combines a microfluidic immunoassay with conventional protein blotting to develop a microfluidic western blot (µWB) (Figure 1e). [19] To realize multiplexed blotting, a parallel, serpentine microfluidic channels chip can dramatically improve the WB system (Figure 1f). [20] This multiplexed micro-blotting Figure 1.…”

Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Materials for Microfluidic Immunoassays: A Review

Mou

Jiang

2017

Adv Healthcare Materials

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118

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Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user‐friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials‐related aspects of the recent advances in microfluidics‐based immunoassays for point‐of‐care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.

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“…We also improved conventional Western blot by integrating it with microfluidics ( Figure 4), incorporating the internal molecular weight marker, loading control, and antibody titration in the same protocol [14]. Compared with the conventional Western blot that could detect only one protein by consuming more than 1 mL of antibody solution in one experiment, the microfluidic Western blot (μWB) can analyze at least 10 proteins simultaneously from a single sample, and it requires only about 1% of the amount of anti-body used in the conventional Western blot.…”

Section: Protein Detection In Microfluidic Systemsmentioning

confidence: 99%

Recent progress in the application of microfluidic systems and gold nanoparticles in immunoassays

Chen

Li²,

et al. 2011

Sci. China Chem.

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Immunoassays are useful for many bioassays. Many new techniques and materials are introduced into the immunoassay to improve the efficiency. This paper reviews recent progress in the application of microfluidic systems and gold nanoparticles in immunoassay. The micro/nano technologies and materials can offer good sensitivity, fast detection, cost-effectiveness and easy signal readout. In particular, the miniaturization of microfluidics and colorimetric assays based on gold nanoparticles have dramatically improved the efficiency of immunoassays.

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Microfluidic Western Blot

Cited by 70 publications

References 29 publications

Organs-on-a-Chip: A Focus on Compartmentalized Microdevices

Organs-on-a-Chip: A Focus on Compartmentalized Microdevices

Materials for Microfluidic Immunoassays: A Review

Recent progress in the application of microfluidic systems and gold nanoparticles in immunoassays

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