Microfluidic viscometer by acoustic streaming transducers

Jiang, Ruoyu; Yoo, Paul D.; Sudarshana, Abhinand Melukote; Mock, Marissa; Pelegri-O’Day, Emma M.; Chhabra, Sandeep; Lee, Abraham P.

doi:10.1039/d3lc00101f

Cited by 3 publications

(1 citation statement)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A falling ball viscometer has been implemented in a microfluidic platform using a photo-interrupter by Kim et al 14 More recently, acoustic techniques have attracted the attention of researchers. 15,16 Electromechanical 17 and optical 18,19 methods are other mechanisms that have been used to measure the fluid viscosity. The complexity of fabrication, instrument adjustment and the need to use external pumps and expensive transducers are some of the drawbacks of these studies.…”

Section: Introductionmentioning

confidence: 99%

Optoelectronic microfluidic device for point-of-care blood plasma viscosity measurement

Bakhtiaridoost,

Musuroi,

Volmer

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Optoelectronic microfluidic device for point-of-care blood plasma viscosity measurement

Bakhtiaridoost,

Musuroi,

Volmer

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

Acoustofluidic Virus Isolation via Bessel Beam Excitation Separation Technology

Xia,

Wang,

Becker

et al. 2024

ACS Nano

View full text Add to dashboard Cite

The isolation of viruses from complex biological samples is essential for creating sensitive bioassays that assess the efficacy and safety of viral therapeutics and vaccines, which have played a critical role during the COVID-19 pandemic. However, existing methods of viral isolation are time-consuming and labor-intensive due to the multiple processing steps required, resulting in low yields. Here, we introduce the rapid, efficient, and highresolution acoustofluidic isolation of viruses from complex biological samples via Bessel beam excitation separation technology (BEST). BEST isolates viruses by utilizing the nondiffractive and self-healing properties of 2D, in-plane acoustic Bessel beams to continuously separate cell-free viruses from biofluids, with high throughput and high viral RNA yield. By tuning the acoustic parameters, the cutoff size of isolated viruses can be easily adjusted to perform dynamic, size-selective virus isolation while simultaneously trapping larger particles and separating smaller particles and contaminants from the sample, achieving high-precision isolation of the target virus. BEST was used to isolate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from human saliva samples and Moloney Murine Leukemia Virus from cell culture media, demonstrating its potential use in both practical diagnostic applications and fundamental virology research. With high separation resolution, high yield, and high purity, BEST is a powerful tool for rapidly and efficiently isolating viruses. It has the potential to play an important role in the development of nextgeneration viral diagnostics, therapeutics, and vaccines.

show abstract

Microfluidic viscometer using capillary pressure sensing

Kang

2023

Physics of Fluids

View full text Add to dashboard Cite

Blood viscosity is considered as a vital determinant of the efficiency of blood flow in blood-vessel networks. The coflowing method is considered as a promising technique for measuring blood viscosity. However, it requires two precise syringe pumps to supply two fluids (i.e., the reference fluid and blood), calibration in advance, and long waiting time for securing steady blood flow. To solve these problems, a single syringe pump is adopted to supply blood into a microfluidic device without requiring a reference fluid. Two key parameters—fluidic resistance and compliance coefficient—are suggested and obtained by analyzing the fluid velocities in a microfluidic channel and calculating the air pressure in the air compliance unit. Using a discrete fluidic circuit model, the pressure difference is analytically derived and utilized as the nonlinear regression formula. The two key parameters are then obtained through nonlinear regression analysis. According to experimental results, the air cavity and flow rate contribute to increasing the compliance coefficient. The fluidic resistance increases significantly at higher concentrations of glycerin solution ranging from 20% to 50%. The proposed method underestimates the values by approximately 27.5% compared with the previous method. Finally, the proposed method is adopted to detect the effects of hematocrit and red blood cell sedimentation in the driving syringe based on two vital parameters. Regarding the fluidic resistance, the normalized difference between the proposed and previous methods is less than 10%. Therefore, two key parameters can be considered as effective for quantitatively monitoring the hematocrit variation in blood flow. In conclusion, from a biomechanical perspective, the proposed method is highly promising for quantifying blood flow in a microfluidic channel.

show abstract

Microfluidic viscometer by acoustic streaming transducers

Abstract: Measurement of fluid viscosity represents a huge need for many biomedical and materials processing applications. Sample fluids containing DNA, antibodies, protein-based drugs, and even cells have become important therapeutic options....

Cited by 3 publications

References 52 publications

Optoelectronic microfluidic device for point-of-care blood plasma viscosity measurement

Optoelectronic microfluidic device for point-of-care blood plasma viscosity measurement

Acoustofluidic Virus Isolation via Bessel Beam Excitation Separation Technology

Microfluidic viscometer using capillary pressure sensing

Contact Info

Product

Resources

About