Microfluidic trap-and-release system for lab-on-a-chip-based studies on giant vesicles

Nuss, Hermann; Chevallard, Corinne; Guénoun, P.; Malloggi, Florent

doi:10.1039/c2lc40782e

Cited by 15 publications

(15 citation statements)

References 35 publications

(52 reference statements)

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“…Features etched in the ceilings of microchannels have been shown to guide and capture water-in-oil droplets, [44,45] but they can also serve as a simple and effective way of trapping GUVs as demonstrated by the Baroud and Bassereau labs. A solution to this is to divert the flow around the trapped vesicles as shown by Nuss et al [46] In their device, tens of GUVs remain trapped at low-hanging beam structures while the main fluid flow is diverted away to one side allowing fast fluidic exchange without loss of trapping. By increasing the flow rates, the vesicles could also be released for off-line analysis.…”

Section: Microstructured Featuresmentioning

confidence: 99%

“…Using this method, reagents can, in theory, be added and removed more rapidly compared to deadend channels, but this could also result in vesicle untrapping. A solution to this is to divert the flow around the trapped vesicles as shown by Nuss et al [46] In their device, tens of GUVs remain trapped at low-hanging beam structures while the main fluid flow is diverted away to one side allowing fast fluidic exchange without loss of trapping. Recently, Yandrapalli et al presented a design able to capture over 23 000 GUVs per device allowing for high-throughput membrane studies (see Figure 2d).…”

Section: Microstructured Featuresmentioning

confidence: 99%

“…As discussed, Nuss et al were able to trap tens in a one location but without a high degree of control over the arrangement or number of vesicles. [46] Future works should take advantage of microfluidic methods to create a specific assembly with a defined number of GUVs which will find its uses in creating tissue-like structures from the bottom-up and also for studying artificial cell-to-cell communication. [97] Moreover, microfluidic handling of large collections of giant unilamellar vesicles as recently presented by the Robinson lab [43] could be adapted for enhanced manipulation and creation of tissue-like systems based on droplet-droplet interfaces (i.e., DIBs).…”

Section: Perspectives For Microfluidic Handling Of Artificial Cellsmentioning

confidence: 99%

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Microfluidic Handling and Analysis of Giant Vesicles for Use as Artificial Cells: A Review

Robinson

2019

Adv. Biosys.

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One of the goals of synthetic biology is the bottom‐up construction of an artificial cell, the successful realization of which could shed light on how cellular life emerged and could also be a useful tool for studying the function of modern cells. Using liposomes as biomimetic containers is particularly promising because lipid membranes are biocompatible and much of the required machinery can be reconstituted within them. Giant lipid vesicles have been used extensively in other fields such as biophysics and drug discovery, but their use as artificial cells has only recently seen an increase. Despite the prevalence of giant vesicles, many experiments remain challenging or impossible due to their delicate nature compared to biological cells. This review aims to highlight the effectiveness of microfluidic technologies in handling and analyzing giant vesicles. The advantages and disadvantages of different microfluidic approaches and what new insights can be gained from various applications are introduced. Finally, future directions are discussed in which the unique combination of microfluidics and giant lipid vesicles can push forward the bottom‐up construction of artificial cells.

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Section: Microstructured Featuresmentioning

confidence: 99%

Section: Microstructured Featuresmentioning

confidence: 99%

Section: Perspectives For Microfluidic Handling Of Artificial Cellsmentioning

confidence: 99%

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Microfluidic Handling and Analysis of Giant Vesicles for Use as Artificial Cells: A Review

Robinson

2019

Adv. Biosys.

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“…The release of trapped droplets has been achieved by flowing an excess continuous phase of oil or by reversing the flow of the trapped droplets. 10,[14][15][16]29,32 In a trapping system for giant unilamellar vesicles (GUVs) the continuous phase has to be increased over a critical value for releasing the trapped GUVs, 33 which is quite similar to another publication. 34 To overcome these limitations, other studies have been made.…”

Section: Introductionmentioning

confidence: 72%

Fast selective trapping and release of picoliter droplets in a 3D microfluidic PDMS multi-trap system with bubbles

Rambach

Biswas

Yadav

et al. 2018

Analyst

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The selective manipulation and incubation of individual picoliter drops in high-throughput droplet based microfluidic devices still remains challenging. We used a surface acoustic wave (SAW) to induce a bubble in a 3D designed multi-trap polydimethylsiloxane (PDMS) device to manipulate multiple droplets and demonstrate the selection, incubation and on-demand release of aqueous droplets from a continuous oil flow. By controlling the position of the acoustic actuation, individual droplets are addressed and selectively released from a droplet stream of 460 drops per s. A complete trapping and releasing cycle can be as short as 70 ms and has no upper limit for incubation time. We characterize the fluidic function of the hybrid device in terms of electric power, pulse duration and acoustic path.

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“…Cells usually retain their shape under shear stress, whereas GUVs can deform which is not always desirable. 19 Hence, trapping using these regular microfluidic cell traps is not efficient as GUVs are lost or pass through geometric traps easily. We solved this issue by diverting the fluid flow around single traps in a circular chamber after the GUVs have been trapped (Fig.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore

et al. 2013

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We present a microfluidic platform able to trap single GUVs in parallel. GUVs are used as model membranes across many fields of biophysics including lipid rafts, membrane fusion, and nanotubes. While their creation is relatively facile, handling and addressing single vesicles remains challenging. The PDMS microchip used herein contains 60 chambers, each with posts able to passively capture single GUVs without compromising their integrity. The design allows for circular valves to be lowered from the channel ceiling to isolate the vesicles from rest of the channel network. GUVs containing calcein were trapped and by rapidly opening the valves, the membrane pore protein a-hemolysin (aHL) was introduced to the membrane. Confocal microscopy revealed the kinetics of the small molecule efflux for different protein concentrations. This microfluidic approach greatly improves the number of experiments possible and can be applied to a wide range of biophysical applications. V C 2013 AIP Publishing LLC.

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Microfluidic trap-and-release system for lab-on-a-chip-based studies on giant vesicles

Cited by 15 publications

References 35 publications

Microfluidic Handling and Analysis of Giant Vesicles for Use as Artificial Cells: A Review

Microfluidic Handling and Analysis of Giant Vesicles for Use as Artificial Cells: A Review

Fast selective trapping and release of picoliter droplets in a 3D microfluidic PDMS multi-trap system with bubbles

Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore

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