Microfluidic tool based on the antibody‐modified paramagnetic particles for detection of 8‐hydroxy‐2′‐deoxyguanosine in urine of prostate cancer patients

Zítka, Ondřej; Křížková, Soňa; Krejčová, Ludmila; Hynek, David; Gumulec, Jaromír; Masařík, Michal; Sochor, Jiří­; Adam, Vojtěch; Hubálek, Jaromír; Trnková, Libuše; Kizek, René

doi:10.1002/elps.201100430

Cited by 28 publications

(24 citation statements)

References 94 publications

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“…Cytosine-adenine reduction (CA peak) was detected by square wave voltammetry coupled with adsorptive transfer technique (AdTS SWV) [45,46,47,48,49,50]. The parameters of the electrochemical determination were as it follows: initial potential 0 V; end potential –1.85 V; frequency 80 Hz; potential step 0.005 V; amplitude 0.025 V. Time of accumulation was optimized.…”

Section: Methodsmentioning

confidence: 99%

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

Krejčová

Hynek

Kopel

et al. 2013

Viruses

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Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads® with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids.

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Section: Methodsmentioning

confidence: 99%

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

Krejčová

Hynek

Kopel

et al. 2013

Viruses

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“…Immunohistochemistry are often used as indirect method for determination of DNA oxidation [38][39][40]. Interaction of antibodies, which have low specificity, or DNA reparation enzyme, is used [39].…”

Section: Determination Of 8-ohdgmentioning

confidence: 99%

“…denaturation [107]. Indirect detection with 1-naphthol generated after 8-OHdG immunoseparation using paramagnetic particles from biological sample has successfully been implemented [40] and it is shown in Fig. Nanoparticles have a lot of physico-chemical advantages [109,110].…”

Section: Perspectives Of 8-ohdg Determination Using Microfluidics Andmentioning

confidence: 99%

Electrochemical Study of DNA Damaged by Oxidation Stress

Zítka¹,

Křížková²,

Skaličková³

et al. 2013

Combinatorial Chemistry & High Throughput Screening

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Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed. The main aim of this review is to report the advantages and features of the electrochemical detection of guanine oxidation product as 8-OHdG and other similarly produced molecules as markers for DNA damage.

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“…This includes 8-hydroxy-2′-deoxyguanosine (8OHdG) which acts as an indicator of DNA oxidation (Ogino and Wang, 2007;Valavanidis et al, 2009;Zwart et al, 1999). Nowadays, the methods used to quantify this biomarker involve complex, expensive and non-portable techniques (Fan et al, 2012;Li et al, 2011;Wang et al, 2011;Zitka et al, 2011), which impair a more effective utilization of OS biomarkers as a predictive tool for degenerative disease installation. Only an immunosensor is presented very recently in literature for 8OHdG, but the overall procedure is very complex (Darwish et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Disposable immunosensor using a simple method for oriented antibody immobilization for label-free real-time detection of an oxidative stress biomarker implicated in cancer diseases

Ferreira

Sales

2014

Biosensors and Bioelectronics

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a b s t r a c tThis work proposes a novel approach for a suitable orientation of antibodies (Ab) on an immunosensing platform, applied here to the determination of 8-hydroxy-2′-deoxyguanosine (8OHdG), a biomarker of oxidative stress that has been associated to chronic diseases, such as cancer. The anti-8OHdG was bound to an amine modified gold support through its Fc region after activation of its carboxylic functions. Nonoriented approaches of Ab binding to the platform were tested in parallel, in order to show that the presented methodology favored Ab/Ag affinity and immunodetection of the antigen.The immunosensor design was evaluated by quartz-crystal microbalance with dissipation, atomic force microscopy, electrochemical impedance spectroscopy (EIS) and square-wave voltammetry. EIS was also a suitable technique to follow the analytical behavior of the device against 8OHdG. The affinity binding between 8OHdG and the antibody immobilized in the gold modified platform increased the charge transfer resistance across the electrochemical set-up. The observed behavior was linear from 0.02 to 7.0 ng/mL of 8OHdG concentrations. The interference from glucose, urea and creatinine was found negligible. An attempt of application to synthetic samples was also successfully conducted.Overall, the presented approach enabled the production of suitably oriented Abs over a gold platform by means of a much simpler process than other oriented-Ab binding approaches described in the literature, as far as we know, and was successful in terms of analytical features and sample application.

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Microfluidic tool based on the antibody‐modified paramagnetic particles for detection of 8‐hydroxy‐2′‐deoxyguanosine in urine of prostate cancer patients

Cited by 28 publications

References 94 publications

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

Electrochemical Study of DNA Damaged by Oxidation Stress

Disposable immunosensor using a simple method for oriented antibody immobilization for label-free real-time detection of an oxidative stress biomarker implicated in cancer diseases

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