Microfluidic Templated Stem Cell Spheroid Microneedles for Diabetic Wound Treatment

Abstract

Wound dressing patches based on stem cells have demonstrated promising potential in the treatment of diabetic wounds, while their capabilities in the maintenance of cell stemness, effective exchange of cellular substance, and precise targeting of the stem cells remain to be promoted. Here, a novel stem cell spheroid loaded microneedle (MN@SPs) patch is presented using microfluidic templating technology. By leveraging the precise fluid manipulation capabilities of the microfluidic template, stem cell spheroids (SPs) with uniform size can be generated in situ. The resulting SPs exhibit advanced viability and cell functions, as evidenced by the overexpressed genes related to extracellular matrix organization and angiogenesis. By loading these SPs into the microneedles (MNs), the platform enables the precise delivery and exchange of multiple active substances, which contribute to advanced neovascularization, collagen deposition, and tissue reconstruction of diabetic wounds. Overall, this microfluidic‐engineered stem cell therapy platform demonstrates significant promise in promoting wound healing.

Multifunctional Microneedle Patch Based on Metal-Phenolic Network with Photothermal Antimicrobial, ROS Scavenging, Immunomodulatory, and Angiogenesis for Programmed Treatment of Diabetic Wound Healing

“…Compared with TA, the C–O stretch in TM shifted from 1208 to 1192 cm –1 and the bending vibration of O–H in TM shifted from 1314 to 1327 cm –1 , suggesting that TA was successfully coordinated with Eu 3+ . All of the above results demonstrate the successful synthesis of TM NPs. , …”

Biological scaffold as potential platforms for stem cells: Current development and applications in wound healing

“…The patch demonstrated excellent efficacy in diabetic wound healing, highlighting the potential value of the proposed composite core-shell microneedle patch for clinical wound healing applications. Wu et al [ 111 ] developed a novel microneedle patch loaded with stem cell spheroids (SPs) using microfluidic templating technology. By incorporating SPs into microneedles, this platform allows for the precise delivery and exchange of multiple active substances, leading to enhanced neovascularization, collagen deposition, and tissue reconstruction in diabetic wounds.…”

Hydrogel-Encapsulated Pancreatic Islet Cells as a Promising Strategy for Diabetic Cell Therapy

“…5 A). Following this, researchers have endeavored to expand the scope of microencapsulation methodologies [ 96 – 98 ]. For instance, experimentation with agarose gels for microencapsulation has been conducted [ 99 ], and xenotransplantation has been explored.…”

“…Despite the progress made, it is still challenging to maintain long-term islet cell activity after microneedle implantation and to load larger amounts of islet cells, so more exploration of microneedles for diabetic glycemic control remains in the area of drug delivery. In recent years, microneedles loaded with ADSC [ 98 , 113 ] and MSC [ 114 ] for diabetic wounds have been attempted in many cases and have made many good advances, which suggests that microneedles encapsulating cells for delivery have a strong potential for the treatment of diabetic complications. All of these provided creative explorations for microencapsulation of pancreatic islet cells.…”