Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae

Bodénès, Pierre; Wang, Hsiang‐Yu; Lee, Tsung-Hua; Chen, Hung-Yu; Wang, Chun-Yen

doi:10.1186/s13068-019-1369-z

Cited by 37 publications

(24 citation statements)

References 144 publications

(225 reference statements)

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“…C. vulgaris has been tested inside microfluidics with different designs for a variety of purposes [18,19,20,21,22]. A microfluidic device (lab-on-a-chip) offers the capability to manipulate a very small volume of fluids; 10 −6 to 10 −18 L through connected microchannels with dimensions from 10 to 100 µm [23].…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions

et al. 2019

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Biodiesel is an eco-friendly renewable fuel that can be derived from microalgae. Maximization of biomass and lipid productivities are considered the main challenges for algal biodiesel production. Since conventional batch cultures are time-, space-, and reagent-consuming with many restrictions to apply many replicates, microfluidic technology has recently emerged as an alternative low-cost and efficient technology with high throughput repeatability and reproducibility. Different applications of microfluidic devices in algal biotechnology have been reported, including cell identification, sorting, trapping, and metabolic screening. In this work, Chlorella vulgaris was investigated by encapsulating in a simple droplet-based micro-array device at different light intensities of 20, 80, and 200 µmol/m2/s combined with different nitrate concentrations of 17.6, 8.8, and 4.4 mM. The growth results for C. vulgaris within microfluidic device were compared to the conventional batch culture method. In addition, the effect of combined stress of deficiencies in irradiance and nitrogen availability were studied to illustrate their impact on the metabolic profiling of microalgae. The results showed that the most optimum favorable culturing conditions for Chlorella vulgaris growth within the microfluidic channels were 17.6 mM and 80 µmol/m2/s.

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Section: Introductionmentioning

confidence: 99%

High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions

et al. 2019

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“…Wang, Zhu, et al, 2019). The advantages and disadvantages of various techniques integrated with microfluidic platforms for microalgal research are detailed with respect to their application areas in Table 1. 2 | SORTING, CULTIVATION, AND HARVESTING VIA MICROFLUIDICS 2.1 | Sorting by shape, size, fluorescence activity, and chemical composition Microalgae can create biofilm structures like bacteria do (Bodenes et al, 2019). The development of biofilm reactors for microalgae is an advantageous way to increase system capacity in large biomass applications required in industrial production.…”

Section: Introductionmentioning

confidence: 99%

Microfluidics for microalgal biotechnology

Özdalgiç

Ustun

Dabbagh

et al. 2021

Biotech & Bioengineering

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Microalgae have expanded their roles as renewable and sustainable feedstocks for biofuel, smart nutrition, biopharmaceutical, cosmeceutical, biosensing, and space technologies. They accumulate valuable biochemical compounds from protein, carbohydrate, and lipid groups, including pigments and carotenoids. Microalgal biomass, which can be adopted for multivalorization under biorefinery settings, allows not only the production of various biofuels but also other value‐added biotechnological products. However, state‐of‐the‐art technologies are required to optimize yield, quality, and the economical aspects of both upstream and downstream processes. As such, the need to use microfluidic‐based devices for both fundamental research and industrial applications of microalgae, arises due to their microscale sizes and dilute cultures. Microfluidics‐based devices are superior to their competitors through their ability to perform multiple functions such as sorting and analyzing small amounts of samples (nanoliter to picoliter) with higher sensitivities. Here, we review emerging applications of microfluidic technologies on microalgal processes in cell sorting, cultivation, harvesting, and applications in biofuels, biosensing, drug delivery, and nutrition.

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“…In this frame, microfluidics and lab-on-a-chip (LOC) developments propose low-cost, multiparameter, real-time, continuous monitoring methods [4,5] while focusing on electrochemical [6] and optical [7] detection techniques. In order to deal with selectivity, beyond the use of several microalgae, the functional integration of electrochemical and optical methods will be required.…”

Section: Introductionmentioning

confidence: 99%

Accurate physiological monitoring using lab-on-a-chip platform for aquatic micro-organisms growth and optimized culture

Belaidi

Salvagnac

Souleille

et al. 2020

Sensors and Actuators B: Chemical

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The present work was dedicated to the development of a lab-on-chip microsystem for the monitoring of microalgal photosynthetic activity. Thanks to integrated electrochemical microcells, dissolved oxygen O2 concentrations due to photosynthetic microalgal activity were measured in a continuous way and oxygen production rates of microalgae cultures were finally determined in the frame of artificial night/day cycles. Application was performed by studying the physiological metabolisms of the green microalga Chlamydomonas reinhardtii. First, the different growth dynamics of microalgal cultures were characterized, enabling the determination of the induction, "exponential growth", "declining growth rate" and stationary phases. Then, the influences of carbon-based nutrients, such as sodium bicarbonate HCO3Na and methanol CH3OH, as well as of pollutants, such as silver nitrate AgNO3, were studied, evidencing contradictory behaviors according to the competition between nutritional properties, toxicity effects and acclimation phenomena. This paves the way to the development of analysis microsystems for the understanding of microalgal metabolisms as well as for the improvement of microalgae growth processes and associated industrial production.

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Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae

Cited by 37 publications

References 144 publications

High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions

High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions

Microfluidics for microalgal biotechnology

Accurate physiological monitoring using lab-on-a-chip platform for aquatic micro-organisms growth and optimized culture

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