Microfluidic Systems with Ion-Selective Membranes

Slouka, Zdeněk; Senapati, Satyajyoti; Chang, Hsueh‐Chia

doi:10.1146/annurev-anchem-071213-020155

Cited by 105 publications

(115 citation statements)

References 94 publications

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“…However, DEP based microfluidic devices especially for our device with 1D nano-slit structures has another advantage of large scale integration for lab-ona-chip device since most of these fabrication methods are based on planar microfabrication. 31 Thus, one can choose the best method for their own applications according to the fabrication facilities and the target entities.…”

Section: Conclusion and Outlooksmentioning

confidence: 99%

On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis

Hui

et al. 2015

Biomicrofluidics

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Electrodeless dielectrophoresis is the best choice to achieve preconcentration of nanoparticles and biomolecules due to its simple, robust, and easy implementation. We designed a simple chip with microchannels and nano-slits in between and then studied the trapping of DNA in high conductive medium and low conductive medium, corresponding to positive and negative dielectrophoresis (DEP), respectively. It is very important to investigate the trapping in media with different conductivities since one always has to deal with the sample solutions with different conductivities. The trapping process was analyzed by the fluorescent intensity changes. The results showed that DNA could be trapped at the nano-slit in both high and low conductive media in a lower electric field strength (10 V/cm) compared to the existing methods. This is a significant improvement to suppress the Joule heating effect in DEP related experiments. Our work may give insight to researchers for DNA trapping by a simple and low cost device in the Lab-on-a-Chip system. V C 2015 AIP Publishing LLC. [http://dx

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Section: Conclusion and Outlooksmentioning

confidence: 99%

On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis

Hui

et al. 2015

Biomicrofluidics

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“…The membrane action essentially establishes de-ionized water condition at the depletion region. 110,111 The same is true for the probe-based capture module. The depletion action of ion-selective membrane still occurs when the main channel is filled with gel, which allows improving the separation and concentration of the nucleic acids in the gel without bulk flow.…”

Section: -9mentioning

confidence: 82%

“…A module that concentrates nucleic acids at a spot by ionic-strength depletion action of a membrane follows. 110,111 This concentration action not only reduces assay time but also shifts the thermodynamics in favor of low-copy numbers. Finally, a probe-functionalized solid-state membrane performs yet another target extraction step with much higher selectivity and is used for quantitative sensing.…”

Section: A Ionic-membrane Integrated Platformmentioning

confidence: 99%

“…From fluorescent measurements, we estimate that despite the robust flow, nucleic acids are concentrated by a factor of 200 with no more than 1% analyte loss. 111 Since the hydrodynamic and electrophoretic mobilities of nucleic acids are size sensitive, by imposing pressure-driven flow onto the concentrated band, larger molecules are separated by flowisotachophoresis. An additional advantage to introducing pressure-driven flow is that, as shear is an irreversible process unencumbered by thermodynamics, the washing action of the high shearrate flow across the probe-based capture unit can enhance selectivity beyond what is permitted by adsorption isotherm for non-targets, making possible efficient removal of non-specifically bound non-targets and single-mismatch discrimination.…”

Section: -9mentioning

confidence: 99%

“…The membrane ion depletion action expands the equilibrium Debye layer into an extended space charge region by osmotic pressure and induces surface microvortex instabilities. 111,114 Mixing due to these vortices results in an overlimiting current and an inflection point in the current-voltage (I-V) curve (Fig. 3(b)).…”

Section: -9mentioning

confidence: 99%

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Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine

et al. 2016

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Nucleic acid biomarkers have enormous potential in non-invasive diagnostics and disease management. In medical research and in the near future in the clinics, there is a great demand for accurate miRNA, mRNA, and ctDNA identification and profiling. They may lead to screening of early stage cancer that is not detectable by tissue biopsy or imaging. Moreover, because their cost is low and they are non-invasive, they can become a regular screening test during annual checkups or allow a dynamic treatment program that adjusts its drug and dosage frequently. We briefly review a few existing viral and endogenous RNA assays that have been approved by the Federal Drug Administration. These tests are based on the main nucleic acid detection technologies, namely, quantitative reverse transcription polymerase chain reaction (PCR), microarrays, and next-generation sequencing. Several of the challenges that these three technologies still face regarding the quantitative measurement of a panel of nucleic acids are outlined. Finally, we review a cluster of microfluidic technologies from our group with potential for point-of-care nucleic acid quantification without nucleic acid amplification, designed to overcome specific limitations of current technologies. We suggest that integration of these technologies in a modular design can offer a low-cost, robust, and yet sensitive/selective platform for a variety of precision medicine applications.

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Designing Angstrom‐Scale Asymmetric MOF‐on‐MOF Cavities for High Monovalent Ion Selectivity

et al. 2022

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Biological ion channels feature angstrom‐scale asymmetrical cavity structures, which are the key to achieving highly efficient separation and sensing of alkali metal ions from aqueous resources. The clean energy future and lithium‐based energy storage systems heavily rely on highly efficient ionic separations. However, artificial recreation of such a sophisticated biostructure has been technically challenging. Here, a highly tunable design concept is introduced to fabricate monovalent ion‐selective membranes with asymmetric sub‐nanometer pores in which energy barriers are implanted. The energy barriers act against ionic movements, which hold the target ion while facilitating the transport of competing ions. The membrane consists of bilayer metal‐organic frameworks (MOF‐on‐MOF), possessing a 6 to 3.4‐angstrom passable cavity structure. The ionic current measurements exhibit an unprecedented ionic current rectification ratio of above 100 with exceptionally high selectivity ratios of 84 and 80 for K+/Li+ and Na+/ Li+, respectively (1.14 Li+ mol m−2 h−1). Furthermore, using quantum mechanics/molecular mechanics, it is shown that the combined effect of spatial hindrance and nucleophilic entrapment to induce energy surge baffles is responsible for the membrane's ultrahigh selectivity and ion rectification. This work demonstrates a striking advance in developing monovalent ion‐selective channels and has implications in sensing, energy storage, and separation technologies.

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Microfluidic Systems with Ion-Selective Membranes

Cited by 105 publications

References 94 publications

On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis

On-chip DNA preconcentration in different media conductivities by electrodeless dielectrophoresis

Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine

Designing Angstrom‐Scale Asymmetric MOF‐on‐MOF Cavities for High Monovalent Ion Selectivity

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