Microfluidic Systems for Whole‐Animal Screening with
            <i>C. elegans</i>

Ghorashian, Navid; Gökçe, Sertan Kutal; Ben‐Yakar, Adela

doi:10.1002/9783527801312.ch10

Cited by 5 publications

(5 citation statements)

References 97 publications

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“…Therefore, the pH 10 solution showed a slight frictional dragging effect in that it deflected slightly in the middle or final stages of the fluid flow patterns with the increase in the fluid flow rate in comparison with that of the pH 4 solution. Nevertheless, the combined flow is laminar owing to the nature of the flow patterns in the internal layers given the non-mixing flow nature of BSs with pH 4 and 10 and the consideration of smaller dimensions 29,30) of the MFC of the MC.…”

Section: Resultsmentioning

confidence: 99%

Terahertz imaging technique for monitoring the flow of buffer solutions at different pH values through a microfluidic chip

Ahmed

Mahana

Taniizumi

et al. 2021

Jpn. J. Appl. Phys.

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Polydimethylsiloxane (PDMS)-based mold prepared using a 3D-printed structure is a cost-effective material and tool to fabricate robust microfluidic chips (MCs) without requiring expensive clean room facilities. A capillary-based MC made of PDMS can be attached onto a glass substrate to visualize the chemical reactions in different types of pH buffer solutions (BSs) flowing through microflow channels (MFCs) using terahertz (THz) image sensing technology. In this study, we designed a microfluidic structure with two inlet wells and an outlet well, equipped with a Si:sapphire substrate to visualize the chemical interaction between BSs injected at different pH values (4 and 10) through an MFC. THz imaging maps were captured during the flow of the BSs using a THz chemical microscope, and the fluid dynamics was studied. We determined and plotted the variation in the THz amplitude data with respect to the BS concentration and analyzed the characteristics of the data.

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Section: Resultsmentioning

confidence: 99%

Terahertz imaging technique for monitoring the flow of buffer solutions at different pH values through a microfluidic chip

Ahmed

Mahana

Taniizumi

et al. 2021

Jpn. J. Appl. Phys.

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“…Pressure-driven platforms currently attract the most research efforts in the microfluidics community, with an average of 30,000 papers per year since 2015 (see Figure 6 for an overview of the publication trend in channel-based microfluidics). Comprehensive surveys cover the existing microfluidics research in cell analysis [55,56,57], Caenorhabditis elegans modeling [58,59,60], organ-on-chip [61,62,63,64], infectious diseases [65] and point of care diagnosis [66,67,68].…”

Section: Current Trends In Microfluidics Researchmentioning

confidence: 99%

Mobile Microfluidics

Alistar

2019

Bioengineering

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Microfluidics platforms can program small amounts of fluids to execute a bio-protocol, and thus, can automate the work of a technician and also integrate a large part of laboratory equipment. Although most microfluidic systems have considerably reduced the size of a laboratory, they are still benchtop units, of a size comparable to a desktop computer. In this paper, we argue that achieving true mobility in microfluidics would revolutionize the domain by making laboratory services accessible during traveling or even in daily situations, such as sport and outdoor activities. We review the existing efforts to achieve mobility in microfluidics, and we discuss the conditions mobile biochips need to satisfy. In particular, we show how we adapted an existing biochip for mobile use, and we present the results when using it during a train ride. Based on these results and our systematic discussion, we identify the challenges that need to be overcome at technical, usability and social levels. In analogy to the history of computing, we make some predictions on the future of mobile biochips. In our vision, mobile biochips will disrupt how people interact with a wide range of healthcare processes, including medical testing and synthesis of on-demand medicine.

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“…Numerous microfluidics‐based solutions were previously proposed for confining worms in a controlled space, where each platform served different purposes and varied in fabrication complexity and experimental operation. [ 136–149 ] In one well‐exploited example, the “Worm Spa” microfluidic device, was used in several applications such as surveillance of C. elegans response to environmental cues, serotonin promotion of feeding dynamics, and longitudinal imaging. [ 150–153 ]…”

Section: Introductionmentioning

confidence: 99%

“…Numerous microfluidicsbased solutions were previously proposed for confining worms in a controlled space, where each platform served different purposes and varied in fabrication complexity and experimental operation. [136][137][138][139][140][141][142][143][144][145][146][147][148][149] In one well-exploited example, the "Worm Spa" microfluidic device, was used in several applications such as surveillance of C. elegans response to environmental cues, serotonin promotion of feeding dynamics, and longitudinal imaging. [150][151][152][153] Despite the significant potential of using NIR imaging with functionalized SWCNTs in C. elegans confined within a microfluidic device, thorough investigations of such a platform that can utilize long-term NIR imaging for in vivo applications are lacking.…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Tracking of Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes in C. Elegans Nematodes Confined in a Microfluidics Platform

Sharaga,

Hendler‐Neumark,

Kamber

et al. 2023

Adv Materials Technologies

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Caenorhabditis elegans (C. elegans) nematodes are a powerful model organism for diverse biological and biomedical studies, benefiting from their genetic similarities to humans, small size, and transparency. However, fluorescence imaging of C. elegans can be challenging due to the strong autofluorescence in the visible range, which obscures the signal of common fluorescent proteins or dyes. Single‐walled carbon nanotubes (SWCNTs) fluoresce in the near‐infrared (NIR) range, where there is no autofluorescence background. Herein, a platform is developed for in vivo NIR imaging of C. elegans gastrointestinal tract using biocompatible SWCNTs functionalized by single‐stranded DNA or phospholipid‐polyethylene glycol (PEG). The SWCNTs serve as fluorescent tracking probes within the worm gut, following internalization along with food intake. A microfluidic confinement device is employed to ensure an anesthetics‐free feeding environment, allowing spatiotemporal control over the SWCNTs intake imaging. Furthermore, improvements in spectral colocalization, real‐time detection of intracorporeal SWCNT dynamics, and digestive trajectory tracking are demonstrated. Owing to the unique optical properties of SWCNTs and the confinement of the worms in the microfluidics system, the proposed platform facilitates advanced in vivo imaging of C. elegans in both the visible and NIR regions, opening numerous avenues for advancing research of C. elegans and other microscopic model organisms.

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Microfluidic Systems for Whole‐Animal Screening with C. elegans

Cited by 5 publications

References 97 publications

Terahertz imaging technique for monitoring the flow of buffer solutions at different pH values through a microfluidic chip

Terahertz imaging technique for monitoring the flow of buffer solutions at different pH values through a microfluidic chip

Mobile Microfluidics

Spatiotemporal Tracking of Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes in C. Elegans Nematodes Confined in a Microfluidics Platform

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