Microfluidic Systems Applied in Solid-State Nanopore Sensors

Abstract

Microfluidic system, as a kind of miniature integrated operating platform, has been applied to solid-state nanopore sensors after many years of experimental study. In the process of introducing microfluidic into solid-state nanopore sensors, many novel device structures are designed due to the abundance of analytes and the diversity of detection methods. Here we review the fundamental setup of nanopore-based microfluidic systems and the developments and advancements that have been taking place in the field. The microfluidic systems with a multichannel strategy to elevate the throughput and efficiency of nanopore sensors are then presented. Multifunctional detection represented by optical-electrical detection, which is realized by microfluidic integration, is also described. A high integration microfluidic system with nanopore is further discussed, which shows the prototype of commercialization.

Nanopore sensors for viral particle quantification: current progress and future prospects

“…At first, the role of microfluidic system in nanopore sensing was to provide a structural platform. However, the recent advancements in microfluidics have resulted in improved performance of microfluidic-based nanopore sensors [ 121 ]. Integration of solid-state nanopores with microfluidics allows analyzing biomolecules while benefiting from sample processing capabilities of microfluidic.…”

“…Also, minimizing the required fluidic tubing and electrode increases the scalability. Integrated microelectrodes with fixed positions provide precise measurement by reducing the noise of the system and the chance of air exposure [ 121 ]. Microfluidics also enables obtaining a high throughput nanopore sensing platform by designing nanopore arrays and performing parallel sensing operations on-chip, leading to a high-throughput sensing platform at the single-molecule level.…”

Microfluidic Technology and Biomedical Field

“…The Lab on a Chip (LOC) concept is a system that combines fluid mechanics, physics and nanotechnology in which large laboratory tests are reduced to a single stage. On-chip sensors integrated with microfluidic devices have great potential in the On-chip laboratory or independent systems for various biological and biomedical applications [12]. The flow of the surface tension in the liquid through the microchannels is triggered due to the change in liquid volumes.…”

Performance Comparison of Flow-Through Optofluidic Biosensor Designs

“…The goal of continued biosensor development is to increase the sensitivity of the device, which for this platform means designing the sensor to collect and transmit a fluorescent signal both high in intensity and with low variance. Some developments in the field of biosensing include the use of nanopores to isolate bio-targets for specific examination [8][9][10][11]. The nanopore allows for the electrical detection of targets, including single-molecule targets, and offers some benefit of controlling the delivery of the sample to the sensing structure of the device [8,11].…”

“…Some developments in the field of biosensing include the use of nanopores to isolate bio-targets for specific examination [8][9][10][11]. The nanopore allows for the electrical detection of targets, including single-molecule targets, and offers some benefit of controlling the delivery of the sample to the sensing structure of the device [8,11]. Nanopore applications can even trap complex nucleic molecules and facilitate translocation of their subcomponents [9].…”