Microfluidic system for cell fusion

Grabowski, Marek; Buchenauer, Andreas; Hasni, Akram El; Klockenbring, Thorsten; Barth, Stefan; Mokwa, W.; Schnakenberg, Uwe

doi:10.1016/j.proeng.2010.09.360

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Since this device relied on cells that were in close contact when PEG was introduced, the fusion efficiency was 28.8% only. In a separate study by Grabowski et al, a microfluidic trap and a pneumatic valve were used to actuate the flow to bring cells into contact, followed by the introduction of PEG to induce fusion . The cells tested were human myeloid cell line U937 and human lymphoid cell line L540.…”

Section: Fusionmentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

et al. 2022

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Microscale surgery on single cells and small organisms has enabled major advances in fundamental biology and in engineering biological systems. Examples of applications range from wound healing and regeneration studies to the generation of hybridoma to produce monoclonal antibodies. Even today, these surgical operations are often performed manually, but they are labor intensive and lack reproducibility. Microfluidics has emerged as a powerful technology to control and manipulate cells and multicellular systems at the micro- and nanoscale with high precision. Here, we review the physical and chemical mechanisms of microscale surgery and the corresponding design principles, applications, and implementations in microfluidic systems. We consider four types of surgical operations: (1) sectioning, which splits a biological entity into multiple parts, (2) ablation, which destroys part of an entity, (3) biopsy, which extracts materials from within a living cell, and (4) fusion, which joins multiple entities into one. For each type of surgery, we summarize the motivating applications and the microfluidic devices developed. Throughout this review, we highlight existing challenges and opportunities. We hope that this review will inspire scientists and engineers to continue to explore and improve microfluidic surgical methods.

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Section: Fusionmentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

et al. 2022

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“…Small volume systems drastically decrease sample and reagent consumption, reduce mixing and reaction times, and are amenable to a high degree of system automation which enables portable, cost-effective, and high-throughput analyses. Microfluidic systems have been widely used in biological analyses, such as polymerase chain reaction (PCR), , DNA analysis, , cell analysis, , and protein separation. , However, the continued development of miniaturized integrated analysis systems requires the development of microfluidic components with superior performance …”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Glass/PDMS Microfluidic Valve Assemblies Enhances Valve Electrical Resistance

Wang

Agasid

Baker

et al. 2019

ACS Appl. Mater. Interfaces

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Microfluidic instrumentation offers unique advantages in biotechnology applications including reduced sample and reagent consumption, rapid mixing and reaction times, and a high degree of process automation. As dimensions decrease, the ratio of surface area to volume within a fluidic architecture increases, which gives rise to some of the unique advantages inherent to microfluidics. Thus, manipulation of surface characteristics presents a promising approach to tailor the performance of microfluidic systems. Microfluidic valves are essential components in a number of small volume applications and for automated microfluidic platforms, but rigorous evaluation of the sealing quality of these valves is often overlooked. In this work, the glass valve seat of hybrid glass/PDMS microfluidic valves was surface modified with hydrophobic silanes, octyldimethylchlorosilane (ODCS) or (tridecafluoro-1,1,2,2-tetrahydrooctyl)dimethylchlorosilane (PFDCS), to investigate the effect of surface energy on electrical resistance of valves. Valves with ODCS-or PFDCS-modified valve seats both exhibited >70-fold increases in electrical resistance (>500 GΩ) when compared to the same valve design with unmodified glass valve seats (7 ± 3 GΩ), indicative of higher sealing capacity. The opening times for valves with ODCS-or PFDCS-modified valve seats was ca. 5× shorter compared to unmodified valve seats, whereas the closing time was up to 8× longer for modified valve seats, although the total closing time was ≤1.5 s, compatible with numerous microfluidic valving applications. Surface modified valve assemblies offered sufficient electrical resistance to isolate sub-pA current signals resulting from electrophysiology measurement of α-hemolysin conductance in a suspended lipid bilayer. This approach is well-suited for the design of novel microfluidic architectures that integrate fluidic manipulations with electrophysiological or electrochemical measurements.

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Microfluidic system for cell fusion

Cited by 2 publications

References 6 publications

Microfluidic Surgery in Single Cells and Multicellular Systems

Microfluidic Surgery in Single Cells and Multicellular Systems

Surface Modification of Glass/PDMS Microfluidic Valve Assemblies Enhances Valve Electrical Resistance

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