Microfluidic Synthesis of Cell-Type-Specific Artificial Extracellular Matrix Hydrogels

Allazetta, Simone; Hausherr, Tanja Cloé; Lütolf, Matthias P.

doi:10.1021/bm4000162

Cited by 83 publications

(97 citation statements)

References 48 publications

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“…[134][135][136][137][138][139][140][141][142][143][144][145][146][147][148][149][150] VS are typically attached to polymer chains via multiple-step reactions, for example, fi rst reacting mercaptoalkanoic acid to divinyl sulfone to yield a free carboxylic acid group and subsequently performing an esterifi cation with hydroxyl groups on a carbohydrate via carbodiimide chemistry. [ 148 ] Single-step functionalizations are also possible via direct reactions between a polymer-bound hydroxyl or amine group and VS in the presence of a strong base [ 138,145 ] or copolymerization of a ring opening monomer possessing VS functionality that provides direct chain functionalization with VS without the need for post-polymerization reactions.…”

Section: Tissue Interactionsmentioning

confidence: 99%

Designing Injectable, Covalently Cross‐Linked Hydrogels for Biomedical Applications

Patenaude

Smeets

Hoare

2014

Macromol. Rapid Commun.

165

125

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Hydrogels that can form spontaneously via covalent bond formation upon injection in vivo have recently attracted significant attention for their potential to address a variety of biomedical challenges. This review discusses the design rules for the effective engineering of such materials, and the major chemistries used to form injectable, in situ gelling hydrogels in the context of these design guidelines are outlined (with examples). Directions for future research in the area are addressed, noting the outstanding challenges associated with the use of this class of hydrogels in vivo.

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Section: Tissue Interactionsmentioning

confidence: 99%

Designing Injectable, Covalently Cross‐Linked Hydrogels for Biomedical Applications

Patenaude

Smeets

Hoare

2014

Macromol. Rapid Commun.

165

125

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“…(Allazetta et al 2013). Further it is feasible to encapsulate cells within agarose to provide a 3D environment for individual cells (Hammill et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Microsphere cytometry to interrogate microenvironment-dependent cell signaling

et al. 2017

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2018Avhandling for graden philosophiae doctor (ph.d.)Dato for disputas: 06.04.18Trykk:Skipnes Kommunikasjon / Universitetet i Bergen Materialet i denne publikasjonen er omfattet av åndsverkslovens bestemmelser. Tittel:The effects of ageing on microenvironment-contextual epithelial cell signalling Rein Aasland, for valuable insight on academic writing, and for pointing out, as did James, when my words became self-indulgent fun and flowers. Mark LaBarge, for taking me into his lab, and letting me partake in that which concerns absolutely everyone: ageing. To Martha Stampfer for allowing me to poke around in her human cell resources biobank. To James Garbe for being an incredibly sympathetic guy with the most convenient knowledge up his sleeve.I would like to thank my mother Nina-Mamma for never curbing her enthusiasm for tiny biology when I brought home science homework, and my father Ulf for being genuinely impressed with pretty much everything I didincluding when I did it badly. I need to thank my boyfriend, Lars, for meticulous proofreading of the manuscript, and for continuously encouraging me to put my academic whims into writing. I want to point out that Iren Abrahamsen did substantial preliminary work for the microsphere cytometry method. I need to to thank my medical doctor office mates, Gry and Kjersti for valuable insights from the clinical realm where lab rats never go, to remind me who we're doing this for in the first place. Thanks to Sissel for being that steady rock in the corner stall of the lab where PhDs and postdocs fly by. I would like to thank the girls in the lab for taking up the fight against cancer cell Fråtse-Frida, and winning it. Finally, I would like to thank the Cancer Society, VilVite, CCBIO and Forskningsdagene for giving me trust, whether it was to develop flow cytometry protocols or popular science shows, or to interrogate the microenvironment I would like thank just anyone else in the lab and the general world who is interested and willing to contemplate big ideas such as the purpose of being. And at last, every single nerd: nerdy boys and nerdy girls. pathway activation levels in HMEC are attenuated with age, and that the diminished signaling magnitude in HMEC from ageing women correlated with reduced probability of activating oncogene-induced senescence.Our results suggest that attenuated cell signaling response may reduce the likelihood of activating oncogene induced senescence, for cells in ageing women. We hypothesize this is the result of age-related changes to the microenvironment that support age-emergent cellular phenotypes with increased cancer susceptibility.

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“…Crosslinking PEG-4MAL macromers can be accomplished with dithiol molecules and does not require free radical initiators, which are detrimental to encapsulated cell health 13 . Similar microfluidic schemes for producing microgels [14][15][16] and encapsulating cells [17][18][19] have recently been reported for widely varying applications, including wound healing, stem cell culture and fundamental studies of cell biology. The versatility of microfluidic cell encapsulation extends to other polymers, including Matrigel 20 , agarose 21 and even multilayer core-shell composites such as collagen cores with alginate shells 22 .…”

Section: Introductionmentioning

confidence: 87%

Parallel droplet microfluidics for high throughput cell encapsulation and synthetic microgel generation

2018

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Cells can be microencapsulated in synthetic hydrogel microspheres (microgels) using droplet microfluidics, but microfluidic devices with a single droplet generating geometry have limited throughput, especially as microgel diameter decreases. Here we demonstrate microencapsulation of human mesenchymal stem cells (hMSCs) in small ( o100 μm diameter) microgels utilizing parallel droplet generators on a two-layer elastomer device, which has 600% increased throughput vs. single-nozzle devices. Distribution of microgel diameters were compared between products of parallel vs. single-nozzle configurations for two square nozzle widths, 35 and 100 μm. Microgels produced on parallel nozzles were equivalent to those produced on single nozzles, with substantially the same polydispersity. Microencapsulation of hMSCs was compared for parallel nozzle devices of each width. Thirty five micrometer wide nozzle devices could be operated at twice the cell concentration of 100 μm wide nozzle devices but produced more empty microgels than predicted by a Poisson distribution. Hundred micrometer wide nozzle devices produced microgels as predicted by a Poisson distribution. Polydispersity of microgels did not increase with the addition of cells for either nozzle width. hMSCs encapsulated on 35 μm wide nozzle devices had reduced viability (~70%) and a corresponding decrease in vascular endothelial growth factor (VEGF) secretion compared to hMSCs cultured on tissue culture (TC) plastic. Encapsulating hMSCs using 100 μm wide nozzle devices mitigated loss of viability and function, as measured by VEGF secretion.

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Microfluidic Synthesis of Cell-Type-Specific Artificial Extracellular Matrix Hydrogels

Cited by 83 publications

References 48 publications

Designing Injectable, Covalently Cross‐Linked Hydrogels for Biomedical Applications

Designing Injectable, Covalently Cross‐Linked Hydrogels for Biomedical Applications

Microsphere cytometry to interrogate microenvironment-dependent cell signaling

Parallel droplet microfluidics for high throughput cell encapsulation and synthetic microgel generation

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