Microfluidic stickers

Bartolo, Denis; Degré, Guillaume; Nghe, Philippe; Studer, Vincent

doi:10.1039/b712368j

Cited by 236 publications

(276 citation statements)

References 26 publications

(34 reference statements)

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“…During the PU particle synthesis, we mixed the PU precursors with a toluene solution at a 1:1 volume ratio. In the 25 experiments, the particles synthesized in PDMS devices were deformed, reflecting a swollen channel (Fig. 4b).…”

Section: Comparison With Pdms-based Flow Lithographymentioning

confidence: 99%

“…After that, PDMS (mixed at 10:1 base:curing agent) was poured on the SIFEL layer to add device thickness and save SIFEL material. In 25 particular, the addition of PDMS to the SIFEL layer can offer the enhanced mechanical strength to the devices, prevent the wrinkling problems during the heat curing, and reduce the fabrication costs 44,51 . The composite layer was further cured at 80 ˚C for 45 min.…”

Section: Synthesis Of Umentioning

confidence: 99%

“…Apart from this disadvantage, PFPE-based SFL can exhibit more performances than the PDMS-based SFL. We quantified the dimensions of particles synthesized in both 25 processes and found negligible differences (sub-micron). Also, we used PFPE-based SFL to synthesize multifunctional particles, showing that the technique can be compatible with the particle synthesis process on the structured microflows.…”

Section: Introductionmentioning

confidence: 99%

“…In one cycle of SFL, photopatternable monomers are introduced to a microchannel, the flow quickly stopped, a brief 25 pulse of UV light creates particles which are then flushed out of the device. During the photopolymerization step, particle geometries are defined by masks and channel topographies.…”

Section: Introductionmentioning

confidence: 99%

“…As PFPE has a lower elastic modulus than PDMS, the PFPE channels can extend lag times in the SFL process and decrease the particle throughput. However, we highlight the 25 PFPE devices are compatible with most organic solvents, allowing for the solvent-based SFL that can achieve particles with higher degree of chemical complexity than the prior PDMSbased SFL. We believe that the PFPE-based SFL can be a simple but powerful technique to create new class of multifunctional 30 particles from organic precursors.…”

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confidence: 99%

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Stop flow lithography in perfluoropolyether (PFPE) microfluidic channels

2014

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5Stop Flow Lithography (SFL) is a microfluidic-based particle synthesis method for creating anisotropic multifunctional particles with applications that range from MEMs to biomedical engineering. Polydimethylsiloxane (PDMS) has been typically used to construct SFL devices as the material enables rapid prototyping of channels with complex geometries, optical transparency, and oxygen permeability. However, PDMS is not compatible with most organic solvents which limit the current range of materials 10 that can be synthesized with SFL. Here, we demonstrate that a fluorinated elastomer, called perfluoropolyether (PFPE), can be an alternative oxygen permeable elastomer for SFL microfluidic flow channels. We fabricate PFPE microfluidic devices with soft lithography and synthesize anisotropic multifunctional particles in the devices via the SFL process -this is the first demonstration of SFL with oxygen lubrication layers in a non-PDMS channel. We benchmark the SFL performance of the PFPE 15 devices by comparing them to PDMS devices. We synthesized particles in both PFPE and PDMS devices under the same SFL conditions and found the difference of particle dimensions was less than a micron. PFPE devices can greatly expand the range of precursor materials that can be processed in SFL because the fluorinated devices are chemically resistant to most organic solvents, an inaccessible class of reagents in PDMS-based devices due to swelling.

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Section: Comparison With Pdms-based Flow Lithographymentioning

confidence: 99%

Section: Synthesis Of Umentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Stop flow lithography in perfluoropolyether (PFPE) microfluidic channels

2014

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Programming Spatiotemporal Patterns with DNA‐Based Circuits

Hofstadt

Gines

Galas

et al. 2021

DNA‐ and RNA‐Based Computing Systems

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Overcoming the Pitfalls of Cytochrome P450 Immobilization through the Use of Fusogenic Liposomes

et al. 2018

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This work describes a new nanotechnology‐based immobilization strategy for cytochrome P450s (CYPs), the major class of drug metabolizing enzymes. Immobilization of CYPs on solid supports provides a significant leap forward compared with soluble enzyme assays by enabling the implementation of through‐flow microreactors for, for example, determination of time‐dependent inhibition. Immobilization of the complex CYP membrane‐protein system is however particularly challenging as the preservation of the authentic enzyme kinetic parameters requires the full complexity of the lipid environment. The developed strategy is based on the spontaneous fusion of biotinylated fusogenic liposomes with lipid bilayers to facilitate the gentle biotinylation of human liver microsomes that incorporate all main natural CYP isoforms. The same process is also feasible for the biotinylation of recombinant CYPs expressed in insect cells, same as any membrane‐bound enzymes in principle. As a result, CYPs could be immobilized on streptavidin‐functionalized surfaces, both those of commercial magnetic beads and customized microfluidic arrays, so that the enzyme kinetic parameters remain unchanged, unlike in previously reported immobilization approaches that often suffer from restricted substrate diffusion to the enzyme's active site and steric hindrances. The specificity and robustness of the functionalization method of customized microfluidic CYP assays are also carefully examined.

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Microfluidic stickers

Cited by 236 publications

References 26 publications

Stop flow lithography in perfluoropolyether (PFPE) microfluidic channels

Stop flow lithography in perfluoropolyether (PFPE) microfluidic channels

Programming Spatiotemporal Patterns with DNA‐Based Circuits

Overcoming the Pitfalls of Cytochrome P450 Immobilization through the Use of Fusogenic Liposomes

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