Microfluidic sperm sorting improves ICSI outcomes in patients with increased values of Double-Strand Breaks in sperm DNA

Lara-Cerrillo, Sandra; Muñoz, Cristina Urda; Heras, Moisés de la Casa; Fernández-Pacheco, Silvia Camacho; Santa, Julio Gijón de la; Lacruz-Ruiz, Tania; Rosado-Iglesias, Candela; Gonçalves-Aponte, Verónica; Liébana, Vicente Badajoz; García-Peiró, Agustín

doi:10.1016/j.androl.2021.10.003

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…186 Recent studies exploring the application of microfluidic sperm selection in men with high SDF confirmed its superiority in selecting sperm with lower SDF levels compared to conventional sperm selection methods. 187,188 However, further studies are still required to confirm its impact on pregnancy and live birth rates.…”

Section: Sperm Selection Methodsmentioning

confidence: 99%

Unraveling the Impact of Sperm DNA Fragmentation on Reproductive Outcomes

Nielsen,

Majzoub,

Esteves

et al. 2023

Semin Reprod Med

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In recent years, there has been a growing interest in identifying subcellular causes of male infertility, and sperm DNA fragmentation (SDF) research has been at the forefront of this focus. DNA damage can occur during spermatogenesis due to faulty chromatin compaction or excessive abortive apoptosis. It can also happen as sperm transit through the genital tract, often induced by oxidative stress. There are several methods for SDF testing, with the sperm chromatin structure assay, terminal deoxynucleotidyl transferase d-UTI nick end labeling (TUNEL) assay, comet assay, and sperm chromatin dispersion test being the most commonly used. Numerous studies strongly support the negative impact of SDF on male fertility potential. DNA damage has been linked to various morphological and functional sperm abnormalities, ultimately affecting natural conception and assisted reproductive technology outcomes. This evidence-based review aims to explore how SDF influences male reproduction and provide insights into available therapeutic options to minimize its detrimental impact.

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Section: Sperm Selection Methodsmentioning

confidence: 99%

Unraveling the Impact of Sperm DNA Fragmentation on Reproductive Outcomes

Nielsen,

Majzoub,

Esteves

et al. 2023

Semin Reprod Med

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“…demonstrates how advances in microfluidic sperm sorting, when used in conjunction with ICSI, significantly improve clinical outcomes by facilitating the selection of sperm with lower DNA fragmentation rates, thus improving the potential for successful fertilization and pregnancy outcomes. 176 In addition, research by Sallam, Hassan et al. re‐evaluates the use of ICSI in the context of non‐male factor infertility, suggesting a nuanced understanding of its benefits beyond conventional use, thereby broadening the horizon of its applicability in ART.…”

Section: Prospects For Icsimentioning

confidence: 99%

Evolution of intracytoplasmic sperm injection: From initial challenges to wider applications

Asada

2024

Reprod Medicine & Biology

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BackgroundIn vitro fertilization (IVF) has revolutionized infertility treatment. Nevertheless, male infertility requires more effective solutions. In 1992, the first‐ever case of human birth via intracytoplasmic sperm injection (ICSI) was reported. ICSI involves microscopically injecting a sperm into an ovum. Successful ICSI has become a reliable therapy for couples facing infertility, a significant milestone. However, it has also introduced various challenges. This study also delves into ethical dilemmas arising from widespread ICSI use.MethodsThis review traces the history of ICSI, presenting pioneering attempts, first successful attempts, and critical reports on account of the initial skepticism toward the technology. The review also focuses on chronological progress until ICSI was recognized as effective and became widely applied.Main findingsThe review reveals that ICSI, although transformative, presents challenges. Successes include addressing male infertility and aiding fertilization. However, concerns arise regarding optimal sperm and embryo selection, genetic mutations, and long‐term health implications. Ethical considerations surrounding ICSI's broad applications also surface.ConclusionsDespite its success and effectiveness, ICSI is still evolving as a therapeutic method. By comprehensively evaluating the historical progress and the current status of ICSI and exploring its future prospects, this study highlights the importance of ICSI in infertility treatment.

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