Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples

Quinn, Molly M.; Jalalian, Liza; Ribeiro, Salustiano; Ona, Katherine; Demirci, Utkan; Cedars, Marcelle I.; Rosen, Mitchell P.

doi:10.1093/humrep/dey239

Cited by 133 publications

(108 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was thought to be due to higher maternal age in Fertile group. The studies regarding the use of microfluidic chips for sperm sorting supported that these chips were able to sort spermatozoa with higher DNA integrity (Quinn et al, ; Schulte et al, ); these devices were introduced to ART market with the claim that their use might be beneficial for decreasing miscarriage rates which were reported to be affected by higher sperm DNA fragmentation (Carlini et al, ; Kumar et al, ). The results of our study showed no significant difference in miscarriage rates between both groups and did not support any advantage of chip over conventional sorting methods in terms of this outcome.…”

Section: Discussionmentioning

confidence: 99%

“…These systems have been integrated into use in the field of assisted reproduction after they were converged into systems that can be used with larger volume samples (Chinnasamy, Behr, & Demirci, ; Samuel et al, ). In addition, there are clinical studies showing that spermatozoa processed with these chips yielded higher DNA integrity and lower DNA damage compared with conventional sorting techniques (Quinn et al, ; Schulte, Chung, Ohl, Takayama, & Smith, ). However, the most important question whether their use is of any advantage in terms of laboratory and clinical IVF/ICSI outcomes still remains controversy.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Does a microfluidic chip for sperm sorting have a positive add‐on effect on laboratory and clinical outcomes of intracytoplasmic sperm injection cycles? A sibling oocyte study

Kalyan¹,

Celik

Okan

et al. 2019

Andrologia

View full text Add to dashboard Cite

The most recent technologies for sperm sorting involve microfluidics. However, the most important question whether their use is of any advantage in terms of laboratory and clinical IVF/ICSI outcomes still remains controversy. In this study, we aimed to investigate whether a microfluidic sperm sorting device (Fertile Plus ® ) has a positive add-on effect on laboratory and clinical outcomes. Sibling oocytes of 81 patients were assigned to two sperm sorting groups including swim up and Fertile Plus ® . All embryos were cultured until day 5/6. Fertilisation, embryo quality and blastocyst development were assessed as primary outcomes among 81 patients; clinical pregnancy, implantation and live birth rates were analysed as secondary outcomes as a subgroup analysis due to transfer cancellations. No statistically significant differences were found between groups in terms of all outcomes analysed in laboratory and clinical terms (p > .05 for all). The results of this study suggest that sorting spermatozoa through Fertile chip does not improve laboratory outcomes significantly and does not seem to have a positive contribution to clinical outcomes. K E Y W O R D Schip, implantation, intracytoplasmic sperm injection, microfluidic, sibling

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Does a microfluidic chip for sperm sorting have a positive add‐on effect on laboratory and clinical outcomes of intracytoplasmic sperm injection cycles? A sibling oocyte study

Kalyan¹,

Celik

Okan

et al. 2019

Andrologia

View full text Add to dashboard Cite

show abstract

“…Intracytoplasmic morphologically selected sperm injection (IMSI) uses high magnification to select the most morphologically normal sperm, as the presence of vacuoles in the nuclear region has been associated with high SDF [244,245]. Other approaches include the physiological intracytoplasmic sperm injection (PICSI), based on sperm binding to hyaluronic acid, and microfluidic devices, allowing sperm migration along microchannels [246][247][248][249].…”

Section: Sperm Processing and Preparationmentioning

confidence: 99%

Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

173

144

View full text Add to dashboard Cite

Sperm DNA integrity is crucial for fertilization and development of healthy offspring. The spermatozoon undergoes extensive molecular remodeling of its nucleus during later phases of spermatogenesis, which imparts compaction and protects the genetic content. Testicular (defective maturation and abortive apoptosis) and post-testicular (oxidative stress) mechanisms are implicated in the etiology of sperm DNA fragmentation (SDF), which affects both natural and assisted reproduction. Several clinical and environmental factors are known to negatively impact sperm DNA integrity. An increasing number of reports emphasizes the direct relationship between sperm DNA damage and male infertility. Currently, several assays are available to assess sperm DNA damage, however, routine assessment of SDF in clinical practice is not recommended by professional organizations. This article provides an overview of SDF types, origin and comparative analysis of various SDF assays while primarily focusing on the clinical indications of SDF testing. Importantly, we report four clinical cases where SDF testing had played a significant role in improving fertility outcome. In light of these clinical case reports and recent scientific evidence, this review provides expert recommendations on SDF testing and examines the advantages and drawbacks of the clinical utility of SDF testing using Strength-Weaknesses-Opportunities-Threats (SWOT) analysis.

show abstract

“…Two of the most explored methods for sperm processing in ART-density gradient centrifugation (DGS) and swim-up (SU)-are investigated in details. Compared to fresh sample, the processed one has lower DNA fragmentation rates [134,135] and lower concentration of ROS regardless which method was used [136,137].…”

Section: Processing Sperm Samples In Vitromentioning

confidence: 99%

The Sperm: Parameters and Evaluation

Milachich¹,

Dyulgerova-Nikolova²

2020

Innovations in Assisted Reproduction Technology

View full text Add to dashboard Cite

Sperm abnormalities are a major factor of human infertility. Since 1987, there are several references in different editions of World Health Organization (WHO) manual defining optimal sperm parameters. Over the years, many reproductive specialists have been constantly debating, suggesting and remodeling the frame values in those guidelines. Semen parameters have a leading role both in natural conception and assisted reproduction technologies (ART) outcomes. Deviations expressed in lower sperm count, impaired motility, abnormal morphology, and high percentage of sperm DNA fragmentation are linked to reduced chances to achieve pregnancy. In cases with low sperm count, severe oligoasthenozoospermia (OA) or azoospermia, karyotyping or evaluation with sperm aneuploidy test (SAT) could be an option and genetic counseling will be necessary if there is an obvious deviation or aberration (e.g., translocation, aneuploidy, etc.). Taking care of lifestyle factors as body mass index (BMI), diets, alcohol intake, smoking, using some additional nutrition and vitamin supplements might affect sperm parameters and contribute to the chances of a couple to conceive.

show abstract

Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples

Abstract: No external funding to declare. Utkan Demirci, PhD is the Co-founder and Scientific Advisor for DxNow Inc., LevitasBio Inc. and Koek Biotech. Mitchell Rosen, MD is a member of the Clinical Advisory Board for DxNow Inc.

Cited by 133 publications

References 36 publications

Does a microfluidic chip for sperm sorting have a positive add‐on effect on laboratory and clinical outcomes of intracytoplasmic sperm injection cycles? A sibling oocyte study

Does a microfluidic chip for sperm sorting have a positive add‐on effect on laboratory and clinical outcomes of intracytoplasmic sperm injection cycles? A sibling oocyte study

Sperm DNA Fragmentation: A New Guideline for Clinicians

The Sperm: Parameters and Evaluation

Contact Info

Product

Resources

About