Microfluidic single‐cell multiomics analysis

Abstract

Cellular heterogeneity is essential to biological processes, such as embryonic development, cell differentiation, and the progression of disease. Recent years have seen the development of a variety of single‐cell multiomics technologies that systemically codetect the genome, epigenome, transcriptome, and proteome for single‐cell heterogeneity evaluation comprehensively. Microfluidics has emerged as a significant tool for single‐cell multiomics techniques, enabling the analysis of the complex regulatory network associated with genome coding, epigenome regulation, and transcriptome/proteome expression in a single cell with increased detection sensitivity, accuracy, throughput, and integration. A review of state‐of‐the‐art microfluidic single‐cell multiomics analysis is presented here. Various microfluidics for isolating single cells are introduced first, highlighting their advantages, disadvantages, and applications in single‐cell sequencing. Then, a comprehensive overview of microfluidic single‐cell multiomics techniques is provided. In addition, a brief introduction of single‐cell multiomics analysis in biological applications and clinical settings will be presented. Finally, we will conclude by discussing the future challenges and prospects of this field.

Construction of Exosome SORL1 Detection Platform Based on 3D Porous Microfluidic Chip and its Application in Early Diagnosis of Colorectal Cancer

“…[26][27][28][29][30][31][32] The microfluidic chip is a technology for precise control of microfluidics in submicron structures. [33,34] The use of microfluidic chips can facilitate the accurate control and detection of microfluids in the micro and nanoscale space. [35] In recent years, microfluidic chip technology has become a research hotspot in the field of biomedicine due to its unique advantageous properties of fast separation of biological particles resulting in good purity, high integration, and low dosage.…”

Injectable smart stimuli-responsive hydrogels: pioneering advancements in biomedical applications

“…Microfluidics is a commonly employed detection technique in the field of biopharmaceutical analysis, playing an important role in applications such as single-cell multi-omics analysis, hemostasis monitoring, thrombus diagnosis, and liquid biopsy of tumor-derived exosomes. [293][294][295] With its downscaled "chip laboratory" on a micrometer scale, microfluidics systems enable precise manipulation of minuscule volumes of fluids, resulting in a substantial reduction in both testing expenses and sample…”

“…Microfluidics is a commonly employed detection technique in the field of biopharmaceutical analysis, playing an important role in applications such as single-cell multi-omics analysis, hemostasis monitoring, thrombus diagnosis, and liquid biopsy of tumor-derived exosomes. 293–295 With its downscaled “chip laboratory” on a micrometer scale, microfluidics systems enable precise manipulation of minuscule volumes of fluids, resulting in a substantial reduction in both testing expenses and sample consumption. Concurrently, microfluidics has emerged as a novel technique to develop spherical droplets with uniform size of the aqueous hydrogel precursor within a continuous oil phase, and plays a vital role in fabricating HMs.…”

A facile single-cell patterning strategy based on harbor-like microwell microfluidics

“…For droplet microfluidics, waterin oil droplets are typically formed by forcing an aqueous cell suspension into a continuous oil phase within microchannels, act as independent miniaturized reactors for isolating and studying single cells [21]. Due to the advantages of high-throughput, minimal cross-contamination [22], parallelization and integration, droplet microfluidics has found applications in various research areas, including cell encapsulation, single-cell analysis, and high-throughput screening [23,24]. However, due to the encapsulation of cells within individual droplets and oil phase complications, it can be challenging to add or replace reagents within droplets during an ongoing experiment, limiting its utility for multi-step reactions or assays [25,26].…”