Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis

Patel, Saurin; Showers, Daniel; Vedantam, Pallavi; Tzeng, Tzuen‐Rong; Qian, Shizhi; Xuan, Xiangchun

doi:10.1063/1.4732800

Cited by 120 publications

(130 citation statements)

References 66 publications

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“…18 The controlling mechanism in the aforementioned handling techniques can be classified in two categories: active methods based on the application of external force fields and passive methods where their functionality is established by harnessing microchannel geometrical effects and nonlinear hydrodynamic forces. In the past decade, extensive investigations have been conducted in order to trap and sort cells and particles using either innovative active techniques, such as dielectrophoresis (DEP), [19][20][21][22] magnetophoresis, 23 acoustophoresis, 24 and optical tweezers, 25 or novel passive approaches, including pinched flow fractionation (PFF), 26 hydrodynamic filtration, 27 biomimetic methods, 28 hydrophoretic focusing, 29 deterministic lateral displacement (DLD), 30 and surface acoustic wave (SAW)-induced streaming. 31 Each of these methods is associated with some advantages and deficiencies which make them preferable in certain applications.…”

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confidence: 99%

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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Focusing and sorting cells and particles utilizing microfluidic phenomena have been flourishing areas of development in recent years. These processes are largely beneficial in biomedical applications and fundamental studies of cell biology as they provide cost-effective and point-of-care miniaturized diagnostic devices and rare cell enrichment techniques. Due to inherent problems of isolation methods based on the biomarkers and antigens, separation approaches exploiting physical characteristics of cells of interest, such as size, deformability, and electric and magnetic properties, have gained currency in many medical assays. Here, we present an overview of the cell/particle sorting techniques by harnessing intrinsic hydrodynamic effects in microchannels. Our emphasis is on the underlying fluid dynamical mechanisms causing cross stream migration of objects in shear and vortical flows. We also highlight the advantages and drawbacks of each method in terms of throughput, separation efficiency, and cell viability. Finally, we discuss the future research areas for extending the scope of hydrodynamic mechanisms and exploring new physical directions for microfluidic applications.

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confidence: 99%

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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“…However, in microfluidic systems, gravitational force is generally neglected. 28 Considering Newton's law, we can write the differential equation describing cell trajectory…”

Section: A Cell Motion Modelmentioning

confidence: 99%

Dielectrophoretic capture of low abundance cell population using thick electrodes

et al. 2015

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Enrichment of rare cell populations such as Circulating Tumor Cells (CTCs) is a critical step before performing analysis. This paper presents a polymeric microfluidic device with integrated thick Carbon-PolyDimethylSiloxane composite (C-PDMS) electrodes designed to carry out dielectrophoretic (DEP) trapping of low abundance biological cells. Such conductive composite material presents advantages over metallic structures. Indeed, as it combines properties of both the matrix and doping particles, C-PDMS allows the easy and fast integration of conductive microstructures using a soft-lithography approach while preserving O 2 plasma bonding properties of PDMS substrate and avoiding a cumbersome alignment procedure. Here, we first performed numerical simulations to demonstrate the advantage of such thick C-PDMS electrodes over a coplanar electrode configuration. It is well established that dielectrophoretic force (F DEP ) decreases quickly as the distance from the electrode surface increases resulting in coplanar configuration to a low trapping efficiency at high flow rate. Here, we showed quantitatively that by using electrodes as thick as a microchannel height, it is possible to extend the DEP force influence in the whole volume of the channel compared to coplanar electrode configuration and maintaining high trapping efficiency while increasing the throughput. This model was then used to numerically optimize a thick C-PDMS electrode configuration in terms of trapping efficiency. Then, optimized microfluidic configurations were fabricated and tested at various flow rates for the trapping of MDA-MB-231 breast cancer cell line. We reached trapping efficiencies of 97% at 20 ll/h and 78.7% at 80 ll/h, for 100 lm thick electrodes. Finally, we applied our device to the separation and localized trapping of CTCs (MDA-MB-231) from a red blood cells sample (concentration ratio of 1:10). V C 2015 AIP Publishing LLC.

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“…are being designed and fabricated, mainly focused at present for biomedical/life science applications but with applicability to environmental monitoring automated lab on a chip systems. [24][25][26][27][28] Whilst the dielectric properties of soil/water composites have been measured in the past, 29-32 manipulation of soils using AC electric fields in microflows has not been reported to our knowledge and the work described in this paper will represent a novel approach to improve bacterial concentrations from contaminating soils using DEP in order to enhance, for example, downstream biosensor detection limits. In this paper, we present the separation of Bacillus atrophaeus (analogous to B. anthracis) from four soil types using electric fields in a microfluidic system.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic sample preparation for environmental monitoring of microorganisms: Soil particle removal

2014

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Detection of pathogens from environmental samples is often hampered by sensors interacting with environmental particles such as soot, pollen, or environmental dust such as soil or clay. These particles may be of similar size to the target bacterium, preventing removal by filtration, but may non-specifically bind to sensor surfaces, fouling them and causing artefactual results. In this paper, we report the selective manipulation of soil particles using an AC electrokinetic microfluidic system. Four heterogeneous soil samples (smectic clay, kaolinitic clay, peaty loam, and sandy loam) were characterised using dielectrophoresis to identify the electrical difference to a target organism. A flow-cell device was then constructed to evaluate dielectrophoretic separation of bacteria and clay in a continous flow through mode. The average separation efficiency of the system across all soil types was found to be 68.7% with a maximal separation efficiency for kaolinitic clay at 87.6%. This represents the first attempt to separate soil particles from bacteria using dielectrophoresis and indicate that the technique shows significant promise; with appropriate system optimisation, we believe that this preliminary study represents an opportunity to develop a simple yet highly effective sample processing system

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Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis

Cited by 120 publications

References 66 publications

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Dielectrophoretic capture of low abundance cell population using thick electrodes

Dielectrophoretic sample preparation for environmental monitoring of microorganisms: Soil particle removal

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