Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples

Phillips, Elizabeth; Moehling, Taylor J.; Ejendal, Karin F.K.; Hoilett, Orlando S.; Byers, Kristin M.; Basing, Laud Anthony; Jankowski, Lauren; Bennett, Jackson; Lin, Li‐Kai; Stanciu, Lia; Linnes, Jacqueline C.

doi:10.1039/c9lc00506d

Cited by 97 publications

(107 citation statements)

References 34 publications

(55 reference statements)

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“…Finally, colorimetric assays have been used to detect influenza A virus, with limits of 3.2 × 10 −3 hemagglutinating units (HAU) per reaction. Phillips et al (2019) designed a microfluidic rapid and autonomous analytical device (microRAAD) which can leverage the wicking abilities of paper membranes and size discriminating pores to isolate HIV virus particles from human blood cells and then utilises pre-dried RT-LAMP reagents to amplify RNA from the virus particles ( Fig. 8 D).…”

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

“…(b) Upon thermally actuating the valves, wash buffer is released to the amplification zone and the RT-LAMP products migrate to the LFIA for (c) test band development. Reproduced with permission from ( Phillips et al, 2019 ). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)…”

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

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Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

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The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the coronavirus disease 2019 (COVID-19) worldwide pandemic. This unprecedented situation has garnered worldwide attention. An effective strategy for controlling the COVID-19 pandemic is to develop highly accurate methods for the rapid identification and isolation of SARS-CoV-2 infected patients. Many companies and institutes are therefore striving to develop effective methods for the rapid detection of SARS-CoV-2 ribonucleic acid (RNA), antibodies, antigens, and the virus. In this review, we summarize the structure of the SARS-CoV-2 virus, its genome and gene expression characteristics, and the current progression of SARS-CoV-2 RNA, antibodies, antigens, and virus detection. Further, we discuss the reasons for the observed false-negative and false-positive RNA and antibody detection results in practical clinical applications. Finally, we provide a review of the biosensors which hold promising potential for point-of-care detection of COVID-19 patients. This review thereby provides general guidelines for both scientists in the biosensing research community and for those in the biosensor industry to develop a highly sensitive and accurate point-of-care COVID-19 detection system, which would be of enormous benefit for controlling the current COVID-19 pandemic.

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Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

339

309

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“…We finish by discussing a published work from Phillips et al for a POC microfluidic rapid and autonomous analysis device (microRAAD) for the detection of the human immunodeficiency virus (HIV) [ 182 ]. The apparatus was built using a laminated and replaceable μPAD, a Kapton tape with printed resistive heating elements, and a temperature control circuit that could connect to a power source like a cell phone or portable battery, all assembled inside a plastic case as shown in Figure 12 a.…”

Section: Applications Of µPadsmentioning

confidence: 99%

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

et al. 2020

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Microfluidic paper-based analytical devices (µPADs) have become promising tools offering various analytical applications for chemical and biological assays at the point-of-care (POC). Compared to traditional microfluidic devices, µPADs offer notable advantages; they are cost-effective, easily fabricated, disposable, and portable. Because of our better understanding and advanced engineering of µPADs, multistep assays, high detection sensitivity, and rapid result readout have become possible, and recently developed µPADs have gained extensive interest in parallel analyses to detect biomarkers of interest. In this review, we focus on recent developments in order to achieve µPADs with high-throughput capability. We discuss existing fabrication techniques and designs, and we introduce and discuss current detection methods and their applications to multiplexed detection assays in relation to clinical diagnosis, drug analysis and screening, environmental monitoring, and food and beverage quality control. A summary with future perspectives for µPADs is also presented.

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“…By reducing the power by more than half (from 3 V to 1.2 V), we also enable a greater ability to incorporate these microheaters into cheap and portable cell phone powered platforms for multi-temperature and sequential heating, which was successful. 55 We hope this work can be built upon using our simple methods to further the potential for accessible diagnostics worldwide.…”

Section: Application-driven Heatingmentioning

confidence: 99%

Versatile printed microheaters to enable low-power thermal control in paper diagnostics

Byers

Lin

Moehling

et al. 2020

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Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples

Abstract: This handheld sample-to-answer platform combines blood cell separation, viral lysis, and isothermal nucleic acid amplification with capillary fluidics and heating controls to automatically detect HIV from blood samples within 90 minutes.

Cited by 97 publications

References 34 publications

Detection of COVID-19: A review of the current literature and future perspectives

Detection of COVID-19: A review of the current literature and future perspectives

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

Versatile printed microheaters to enable low-power thermal control in paper diagnostics

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