Microfluidic Probes and Quadrupoles: A new era of open microfluidics

Brimmo, Ayoola T.; Qasaimeh, Mohammad A.

doi:10.1109/mnano.2016.2633678

Cited by 16 publications

(8 citation statements)

References 59 publications

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“…293,294 The stagnation point ow generated by the four-aperture MFP is typically referred to as the microuidic quadrupole (MQ). 284,[295][296][297] The ow eld of the conventional MQ is reminiscence of the classical quadrupolar electrostatic eld (see Fig. 8f), and is also very similar to that generated by the four-roll mill and the cross-slot.…”

Section: Microuidic Probesmentioning

confidence: 98%

Stagnation point flows in analytical chemistry and life sciences

Brimmo

Qasaimeh

2017

RSC Adv.

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Section: Microuidic Probesmentioning

confidence: 98%

Stagnation point flows in analytical chemistry and life sciences

Brimmo

Qasaimeh

2017

RSC Adv.

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“…[206,207] Detailed reviews on the application of microfluidic quadrupoles can be found in ref. [208].…”

Section: Microparticle Tweezersmentioning

confidence: 99%

Recent Advances and Perspectives in Microfluidics‐Based Single‐Cell Biosensing Techniques

Brimmo

Qasaimeh

et al. 2017

Small Methods

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Single‐cell analyses of secretory proteins are essential to fully understand cellular functional heterogeneity and unravel the underlying mechanisms of intercellular signaling and interactions. Retrieving dynamic information of protein secretion at single‐cell resolution reflects the precise, real‐time functional states of individual cells in physiological processes. Such measurements remain very challenging in single‐cell analysis, which requires highly integrated systems capable of performing on‐chip single‐cell isolation and subsequent real‐time protein detection. Here, recent advances in microfluidics‐based single‐cell manipulation and emerging approaches for label‐free single‐cell biosensing are reviewed. The advantages and limitations of these technologies are summarized and challenges to establish the integrated microfluidic biosensing systems for real‐time single‐cell secretomics are discussed. Recent efforts on integrated platforms for on‐chip single‐cell protein assays are highlighted and some perspectives on future directions in this field are provided.

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“…In our system‐exemplified by the concept of the microfluidic probe (MFP) [ 27 ] —the probe and the substrate are fully decoupled, where the hydrodynamic flow confinement (HFC) provides “virtual walls” to confine the processed fluid. [ 28 ] The open configuration also allows easy access of cells (by simply pulling the probe away from substrate) for postcapture analysis. [ 29 ] Meanwhile, the MFP comes with ability to scan over the capture substrate in a 2D printing mode, which brings multiplexed cell capture functionality.…”

Section: Introductionmentioning

confidence: 99%

Herringbone Microfluidic Probe for Multiplexed Affinity‐Capture of Prostate Circulating Tumor Cells

Glia

Deliorman

Sukumar

et al. 2021

Adv Materials Technologies

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In this work, for first time, circulating tumor cells (CTCs) are captured on an open biofunctionalized substrate with multiplexing capability. This is achieved by developing a new microfluidic probe (MFP) integrated with radially staggered herringbone (HB) elements for microvortex generation. The new tool, named as herringbone microfluidic probe (HB‐MFP), is a channel‐less microfluidic system with physically separated bottom capture substrate and top fluidics delivery system. The concept allows for functionalizing the capture substrate with multiple biorecognition ligands (in this work, stripes of different capture antibodies) and scanning the fluidics delivery system across the substrate in a 2D printing‐like movement. Using the HB‐MFP, CTCs are efficiently captured from prostate cancer blood samples through their specific EpCAM, PSMA, and PSA antigens in a single run, with counts ranging from as low as 6 CTCs mL‐1 (localized cancer patients) to as high as 280 CTCs mL‐1 (metastatic cancer patients). In the process, CTC clusters with sizes of as high as 40–50 cells are also successfully captured. The results indicate that multiplex profiles of CTCs could reveal certain cellular phenotypes based on PSMA and PSA expression levels. The developed HB‐MFP is simple and robust to use, allows for high throughput sample processing, and provides seamless access to captured CTCs for further downstream characterization.

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Microfluidic Probes and Quadrupoles: A new era of open microfluidics

Cited by 16 publications

References 59 publications

Stagnation point flows in analytical chemistry and life sciences

Stagnation point flows in analytical chemistry and life sciences

Recent Advances and Perspectives in Microfluidics‐Based Single‐Cell Biosensing Techniques

Herringbone Microfluidic Probe for Multiplexed Affinity‐Capture of Prostate Circulating Tumor Cells

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