Microfluidic PMMA‐based microarray sensor chip with imaging analysis for ABO and RhD blood group typing

Pipatpanukul, Chinnawut; Amarit, Ratthasart; Somboonkaew, Armote; Sutapun, Boonsong; Vongsakulyanon, Apirom; Kitpoka, Pimpun; Srikhirin, Toemsak; Kunakorn, Mongkol

doi:10.1111/vox.12313

Cited by 11 publications

(11 citation statements)

References 22 publications

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“…Additionally, in one study, ABO and RH(D) blood groups were detected using an antibody microarray on the poly (methylmethacrylate) surface coupled with the microfluidic system (52). The Poly (methylmethacrylate) microarray chip showed good reproducibility, accuracy, and precision.…”

Section: Microfluidic Testingmentioning

confidence: 99%

Blood Group Testing

Guo

2022

Front. Med.

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Red blood cell (RBC) transfusion is one of the most frequently performed clinical procedures and therapies to improve tissue oxygen delivery in hospitalized patients worldwide. Generally, the cross-match is the mandatory test in place to meet the clinical needs of RBC transfusion by examining donor-recipient compatibility with antigens and antibodies of blood groups. Blood groups are usually an individual's combination of antigens on the surface of RBCs, typically of the ABO blood group system and the RH blood group system. Accurate and reliable blood group typing is critical before blood transfusion. Serological testing is the routine method for blood group typing based on hemagglutination reactions with RBC antigens against specific antibodies. Nevertheless, emerging technologies for blood group testing may be alternative and supplemental approaches when serological methods cannot determine blood groups. Moreover, some new technologies, such as the evolving applications of blood group genotyping, can precisely identify variant antigens for clinical significance. Therefore, this review mainly presents a clinical overview and perspective of emerging technologies in blood group testing based on the literature. Collectively, this may highlight the most promising strategies and promote blood group typing development to ensure blood transfusion safety.

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Section: Microfluidic Testingmentioning

confidence: 99%

Blood Group Testing

Guo

2022

Front. Med.

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“…This technology is widely used in the fields of genomics and proteomics. Solidphase antibody microarrays were fabricated for ABO and RhD blood typing [39] . Using image recognition, this array chip demonstrated good accuracy and precision for rapid blood grouping.…”

Section: Microarray Technologymentioning

confidence: 99%

Microchip technology applications for blood group analysis

Gao¹,

Misevic²

2020

Blood and Genomics

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Blood group analysis techniques are some of the most in demand immunological applications in clinical transfusion praxis and organ transplantation. In order to aid the advance towards higher throughput and increased sensitivity, analytical solutions dealing with a minimal amount of blood samples and the miniaturization of diagnostic equipment using microchip technologies have been evolving into an optimal solution. Here we review fabrication technologies for various types of microstructure on microchips, related operating procedures, and characterization approaches. Our focus is on examples of microchip technology and instrumentation used for blood group analysis ranging from classical serological methods of glycoprotein detection and solid phase assays, to nucleic acid amplification techniques. Molecular typing using microchip-based techniques is emerging as a supplement to standard serological methods. Microchip technology will play its key role to support blood group analysis at the molecular scale by using microliters of blood samples for extremely sensitive, quantitative, and high throughput analyses.

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“…Polymer substrates include bulk polymer materials such as plastic chips or slides, and thin polymer films deposited on a solid substrate. Plastic chips are desirable for high-throughput analysis because of cost and flexibility in design. − Microfluidic devices are commonly fabricated from polymer materials and have been integrated into microarray technology. − Additionally, the generation of microarrays in standard lab equipment such as plastic microwell plates would allow a simple interfacing to standard lab automation systems. Nonspecific protein adsorption is a ubiquitous phenomenon for the hydrophobic surfaces of plastics and polymers and needs to be considered …”

Section: Immobilization Chemistries For Microarray Surfacesmentioning

confidence: 99%

The Surface Science of Microarray Generation–A Critical Inventory

Brittain

Brandsetter

Prucker

et al. 2019

ACS Appl. Mater. Interfaces

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Microarrays are powerful tools in biomedical research and have become indispensable for high-throughput multiplex analysis, especially for DNA and protein analysis. The basis for all microarray processing and fabrication is surface modification of a chip substrate and many different strategies to couple probe molecules to such substrates have been developed. We present here a critical assessment of typical biochip generation processes from a surface science point of view. While great progress has been made from a molecular biology point of view on the development of qualitative assays and impressive results have been obtained on the detection of rather low concentrations of DNA or proteins, quantitative chip-based assays are still comparably rare. We argue that lack of stable and reliable deposition chemistries has led in many cases to suboptimal quantitative reproducibility, impeded further progress in microarray development and prevented a more significant penetration of microarray technology into the diagnostic market. We suggest that surface-attached hydrogel networks might be a promising strategy to achieve highly sensitive and quantitatively reproducible microarrays.

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Microfluidic PMMA‐based microarray sensor chip with imaging analysis for ABO and RhD blood group typing

Abstract: The PMMA array chip demonstrated its good accuracy and precision in rapid blood group testing. For its high throughput, the method has potential for use in large blood donation centre.

Cited by 11 publications

References 22 publications

Blood Group Testing

Blood Group Testing

Microchip technology applications for blood group analysis

The Surface Science of Microarray Generation–A Critical Inventory

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