Microfluidic Platforms for the Interrogation of Intravascular Cellular Trafficking Mechanisms Influenced by Hemodynamic Forces

McClatchey, P. Mason; Hannen, Erin; Thomas, Susan N.

doi:10.1007/978-3-319-20726-1_9

Cited by 1 publication

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“…Collectively, these forces influence circulating cell distribution and interactions, leading to the initiation of rolling/tethering adhesion and eventual arrest and infiltration into the surrounding tissue bed. Accordingly, model fluidic systems have been, and continue to be, widely employed in the study of intravascular cell homing for the elucidation of contributing biological and mechanical factors [1,2]. Microfluidic approaches have not only allowed for the identification of important molecular mediators of pathological cell homing, such as in inflammation [3][4][5] and metastasis [6][7][8][9][10][11][12], but have also enabled the study of how hemodynamic forces can affect circulating cell interactions with the vessel wall via these molecular mediators.…”

Section: Introductionmentioning

confidence: 99%

Force and torque on spherical particles in micro-channel flows using computational fluid dynamics

et al. 2016

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To delineate the influence of hemodynamic force on cell adhesion processes, model in vitro fluidic assays that mimic physiological conditions are commonly employed. Herein, we offer a framework for solution of the three-dimensional Navier–Stokes equations using computational fluid dynamics (CFD) to estimate the forces resulting from fluid flow near a plane acting on a sphere that is either stationary or in free flow, and we compare these results to a widely used theoretical model that assumes Stokes flow with a constant shear rate. We find that while the full three-dimensional solutions using a parabolic velocity profile in CFD simulations yield similar translational velocities to those predicted by the theoretical method, the CFD approach results in approximately 50% larger rotational velocities over the wall shear stress range of 0.1–5.0 dynes cm−2. This leads to an approximately 25% difference in force and torque calculations between the two methods. When compared with experimental measurements of translational and rotational velocities of microspheres or cells perfused in microfluidic channels, the CFD simulations yield significantly less error. We propose that CFD modelling can provide better estimations of hemodynamic force levels acting on perfused microspheres and cells in flow fields through microfluidic devices used for cell adhesion dynamics analysis.

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