Microfluidic platforms for single neuron analysis

Gupta, Pallavi; Shinde, Ashwini; Illath, Kavitha; Kar, Siddhartha; Nagai, Masao; Tseng, Fan‐Gang; Santra, Tuhin Subhra

doi:10.1016/j.mtbio.2022.100222

Cited by 15 publications

(8 citation statements)

References 120 publications

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“…Due to its ease of fabrication and its biocompatible properties, polydimethylsiloxane (PDMS)-based microfluidics is of increasing relevance for developmental biology [ 14 ] and has been widely applied for the investigation of various small organisms ranging from plant specimens [ 15 , 16 , 17 ] to animal models [ 18 , 19 , 20 , 21 , 22 ]. Furthermore, microfluidic-based cultivation has recently been reported as a suitable tool for the study of the filamentous growth of the brown alga Ectocarpus silicosus [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Cultivation and Imaging of S. latissima Embryo Monolayered Cell Sheets Inside Microfluidic Devices

et al. 2022

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The culturing and investigation of individual marine specimens in lab environments is crucial to further our understanding of this highly complex ecosystem. However, the obtained results and their relevance are often limited by a lack of suitable experimental setups enabling controlled specimen growth in a natural environment while allowing for precise monitoring and in-depth observations. In this work, we explore the viability of a microfluidic device for the investigation of the growth of the alga Saccharina latissima to enable high-resolution imaging by confining the samples, which usually grow in 3D, to a single 2D plane. We evaluate the specimen’s health based on various factors such as its growth rate, cell shape, and major developmental steps with regard to the device’s operating parameters and flow conditions before demonstrating its compatibility with state-of-the-art microscopy imaging technologies such as the skeletonisation of the specimen through calcofluor white-based vital staining of its cell contours as well as the immunolocalisation of the specimen’s cell wall. Furthermore, by making use of the on-chip characterisation capabilities, we investigate the influence of altered environmental illuminations on the embryonic development using blue and red light. Finally, live tracking of fluorescent microspheres deposited on the surface of the embryo permits the quantitative characterisation of growth at various locations of the organism.

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Section: Resultsmentioning

confidence: 99%

Cultivation and Imaging of S. latissima Embryo Monolayered Cell Sheets Inside Microfluidic Devices

et al. 2022

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“…While the single-cell analysis of other somatic cells (e.g., neurons or endothelial cells) can also provide an essential biological tool for understanding fundamental physiological processes and tissue-specific functions, establishing these studies on integrated microsystems can be limited by technical challenges. [205] Most somatic cells, in contrast to CTCs and WBCs, need to adhere to a solid surface to remain viable and proliferate. [136,138] This commonly requires the tailoring of the integrated microfluidic systems to allow for cell attachment (e.g., ECM protein coating), long-term on-chip cell culture, as well as the detachment of the cells for downstream single-cell analyses.…”

Section: Other Somatic Cellsmentioning

confidence: 99%

“…While the single‐cell analysis of other somatic cells (e.g., neurons or endothelial cells) can also provide an essential biological tool for understanding fundamental physiological processes and tissue‐specific functions, establishing these studies on integrated microsystems can be limited by technical challenges. [ 205 ]…”

Section: Recent Advances In Integrated Single‐cell Separation and On‐...mentioning

confidence: 99%

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Kutluk,

Viefhues,

Constantinou

2024

Small Science

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From deciphering infection and disease mechanisms to identifying novel biomarkers and personalizing treatments, the characteristics of individual cells can provide significant insights into a variety of biological processes and facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited in terms of cost, contamination risks, sample volumes, analysis times, throughput, sensitivity, and selectivity. Although microfluidic approaches have been suggested as a low‐cost, information‐rich, and high‐throughput alternative to conventional single‐cell isolation and analysis methods, limitations such as necessary off‐chip sample pre‐ and post‐processing as well as systems designed for individual workflows have restricted their applications. In this review, a comprehensive overview of recent advances in integrated microfluidics for single‐cell isolation and on‐chip analysis in three prominent application domains are provided: investigation of somatic cells (particularly cancer and immune cells), stem cells, and microorganisms. Also, the use of conventional cell separation methods (e.g., dielectrophoresis) in unconventional or novel ways, which can advance the integration of multiple workflows in microfluidic systems, is discussed. Finally, a critical discussion related to current limitations of integrated microfluidic single‐cell workflows and how they could be overcome is provided.

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“…For CNS studies, compartmentalization is a widely-used fabrication approach to create in vitro neural microfluidics [93]. In the multicompartment microfluidic chips, the presynaptic structure and postsynaptic structure, neuronal soma and axons, as well as different types of cells are segregated in different chambers for the purpose of exploring synapse formation, neural circuits, projection pathway and CNS microenvironment [93,94]. For PNS researches, microscaled channels and grooves are usually integrated to guide axonal growth, which enabled the investigation of peripheral nerve elongation, axonal transport and myelination [19,95].…”

Section: Current Modeling and Application Of Neural System-on-a-chip ...mentioning

confidence: 99%

Microfabrication and lab-on-a-chip devices promote in vitro modeling of neural interfaces for neuroscience researches and preclinical applications

Liu,

Yao,

Fan

et al. 2023

Biofabrication

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Neural tissues react to injuries through the orchestration of cellular reprogramming, generating specialized cells and activating gene expression that helps with tissue remodeling and homeostasis. Simplified biomimetic models are encouraged to amplify the physiological and morphological changes during neural regeneration at cellular and molecular levels. Recent years have witnessed growing interest in lab-on-a-chip technologies for the fabrication of neural interfaces. Neural system-on-a-chip devices are promising in vitro microphysiological platforms that replicate the key structural and functional characteristics of neural tissues. Microfluidics and microelectrode arrays are two fundamental techniques that are leveraged to address the need for microfabricated neural devices. In this review, we explore the innovative fabrication, mechano-physiological parameters, spatiotemporal control of neural cell cultures and chip-based neurogenesis. Although the high variability in different constructs, and the restriction in experimental and analytical access limit the real-life applications of microphysiological models, neural system-on-a-chip devices have gained considerable translatability for modeling neuropathies, drug screening and personalized therapy.

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Microfluidic platforms for single neuron analysis

Cited by 15 publications

References 120 publications

Cultivation and Imaging of S. latissima Embryo Monolayered Cell Sheets Inside Microfluidic Devices

Cultivation and Imaging of S. latissima Embryo Monolayered Cell Sheets Inside Microfluidic Devices

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Microfabrication and lab-on-a-chip devices promote in vitro modeling of neural interfaces for neuroscience researches and preclinical applications

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