Microfluidic Passive Permeability Assay using Nanoliter Droplet Interface Lipid Bilayers

Nisisako, Takasi; Portonovo, Shiva A.; Schmidt, Jacob J.

doi:10.1016/j.bpj.2012.11.3734

Cited by 3 publications

(6 citation statements)

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“…Caffeine is known to passively cross biological membranes [21], [46]. Its permeation coefficient through dioleyl-PC bilayers is about 5 10 2   cm/s [20]. For proflavine, to our knowledge no permeability value is available in the literature.…”

Section: Accepted Manuscriptmentioning

confidence: 98%

“…The permeation coefficient of the membrane takes into account the partition coefficient w lip P / , but also the diffusion coefficient of the drug through the lipid wall. Bilayer permeation coefficients can be determined from liposomes or planar bilayers [45], [47], or more recently in droplet interface bilayer microfluidic assays [48], [49], [20], [50]. For glucose, the permeation coefficients reported for phospholipid bilayers are of the order of 10 10  cm/s.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…In this study, we thoroughly investigate the efficiency of the control of the release rate by the structure for different drugs in various LLC surfactant-oil systems. The model drugs were selected for their different hydrophilicities: caffeine, a wide spread stimulant of the central nervous system [20], [21], [22], the antiseptic dye proflavine [15], and glucose, a model analyte used in a number of release studies from LLC systems [5], [6], [22]. Phosphatidylcholine (PC) and monolinoleine (MLO) were used as LLC surfactants, limonene, cyclohexane and tocopherol as apolar phase modifiers [12], [23], [24], [25], [26], [27], [28], [29].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oil and drug control the release rate from lyotropic liquid crystals

Martiel

Baumann

Vallooran

et al. 2015

Journal of Controlled Release

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Section: Accepted Manuscriptmentioning

confidence: 98%

Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oil and drug control the release rate from lyotropic liquid crystals

Martiel

Baumann

Vallooran

et al. 2015

Journal of Controlled Release

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“…The Laplace pressure at the entrance of the draining channels prevented the drops from entering these side channels under the applied flow conditions. 23–25 The outlet of the draining channels was connected to a polyethylene tubing open to atmosphere. To quantify the draining rate, we measured the volume of the initial continuous phase (rhodamine-doped F-SiO 2 NPs in HFE) in a polyethylene tubing having a known cross-section area within a certain time period.…”

Section: Methodsmentioning

confidence: 99%

“…The Laplace pressure at the entrance of the draining channels prevented the drops from entering these side channels under the applied flow conditions. [23][24][25] The outlet of the draining channels was connected to a polyethylene tubing open to atmosphere.…”

Section: Design Of the Exchangermentioning

confidence: 99%

High-Efficiency and High-Throughput On-Chip Exchange of the Continuous Phase in Droplet Microfluidic Systems

et al. 2017

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This article describes an integrated platform for the on-chip exchange of the continuous phase in droplet microfluidic systems. The drops used in this work are stabilized by amphiphilic nanoparticles. For some characterizations and applications of these nanoparticle-stabilized drops, including the measurement of adsorption dynamics of nanoparticles to the droplet surface, it is necessary to change the composition of the continuous phase from that used during the droplet generation process. Thus far, no work has reported the exchange of the continuous phase for a large number (>1 million) of drops in a microfluidic system. This article describes the design and characterization of a high-efficiency and high-throughput on-chip exchanger of the continuous phase in a continuous-flow droplet microfluidic system. The efficiency of exchange was higher than 97%. The throughput was greater than 1 million drops/min, and this can be increased further by increasing the number of parallel exchangers used. Because drops are injected into the exchanger in a continuous-flow manner, the method is directly compatible with automation to further increase its reliability and potential scale-up.

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Droplet Microfluidics for Food and Nutrition Applications

et al. 2021

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Droplet microfluidics revolutionizes the way experiments and analyses are conducted in many fields of science, based on decades of basic research. Applied sciences are also impacted, opening new perspectives on how we look at complex matter. In particular, food and nutritional sciences still have many research questions unsolved, and conventional laboratory methods are not always suitable to answer them. In this review, we present how microfluidics have been used in these fields to produce and investigate various droplet-based systems, namely simple and double emulsions, microgels, microparticles, and microcapsules with food-grade compositions. We show that droplet microfluidic devices enable unprecedented control over their production and properties, and can be integrated in lab-on-chip platforms for in situ and time-resolved analyses. This approach is illustrated for on-chip measurements of droplet interfacial properties, droplet–droplet coalescence, phase behavior of biopolymer mixtures, and reaction kinetics related to food digestion and nutrient absorption. As a perspective, we present promising developments in the adjacent fields of biochemistry and microbiology, as well as advanced microfluidics–analytical instrument coupling, all of which could be applied to solve research questions at the interface of food and nutritional sciences.

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Microfluidic Passive Permeability Assay using Nanoliter Droplet Interface Lipid Bilayers

Cited by 3 publications

References 0 publications

Oil and drug control the release rate from lyotropic liquid crystals

Oil and drug control the release rate from lyotropic liquid crystals

High-Efficiency and High-Throughput On-Chip Exchange of the Continuous Phase in Droplet Microfluidic Systems

Droplet Microfluidics for Food and Nutrition Applications

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