Microfluidic membrane suppressor module design and evaluation for capillary ion chromatography

Wouters, Sam; Bruggink, Cees; Agroskin, Yury; Pohl, Christopher A.; Eeltink, Sebastiaan

doi:10.1016/j.chroma.2016.12.078

Cited by 9 publications

(7 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The μEMPIS design is based on an earlier described microfluidic ion suppressor for ion chromatography [ 21 ], modified to accommodate multiple parallel channels to increase its ion-exchange capacity. This ion stripper device features a bifurcating channel layout in which the effluent flow is equally distributed over eight microfluidic channels with dimensions of 60 mm × 200 μm × 300 μm (length × depth × width).…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we have developed a single-channel microfluidic membrane suppressor for use in capillary ion chromatography [ 21 , 22 ]. In this study, we describe the design and application of a multichannel microfluidic suppressor (μEMPIS: microfluidic ESI-enabling Mobile Phase Ion-Strip) for the selective removal of TFA ions from LC mobile phases, and exchange with propionic acid and formic acid.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins

et al. 2021

Self Cite

View full text Add to dashboard Cite

Trifluoroacetic acid (TFA) is commonly used as mobile phase additive to improve retention and peak shape characteristics in hydrophilic interaction liquid chromatography (HILIC) of intact proteins. However, when using electrospray ionization-mass spectrometry (ESI-MS) detection, TFA may cause ionization suppression and adduct formation, leading to reduced analyte sensitivity. To address this, we describe a membrane-based microfluidic chip with multiple parallel channels for the selective post-column removal of TFA anions from HILIC. An anion-exchange membrane was used to physically separate the column effluent from a stripper flow solution comprising acetonitrile, formic acid, and propionic acid. The exchange of ions allowed the post-column removal of TFA used during HILIC separation of model proteins. The multichannel design of the device allows the use of flow rates of 0.2 mL/min without the need for a flow splitter, using mobile phases containing 0.1% TFA (13 mM). Separation selectivity and efficiency were maintained (with minor band broadening effects) while increasing the signal intensity and peak areas by improving ionization and reducing TFA adduct formation. Graphical abstract

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nowadays, due to their advantages in terms of portability and high efficiency, microfluidic devices receive increasing attention in many fields, such as biological and healthcare measurement [ 1 , 2 , 3 , 4 , 5 ], chemistry analysis [ 6 , 7 , 8 , 9 , 10 ], and pollution analysis [ 11 ]. Electrical conductivity is a basic and important property of electrolyte solution, biomaterial or fluid [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…As conductivity is proportional to the ion concentration of the solution (low concentration solution), a C 4 D sensor with an LOD greater than 0.2 mS/cm cannot meet the requirements of many applications concerning ion detection at low concentrations [ 16 , 17 , 18 ]. For example, ion chromatograph (IC) is an important method for ion separation and detection, and has been used in the field of microfluidic devices [ 6 , 7 ]. In IC systems, conductivity detectors are the most common detectors [ 6 , 7 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

A Low Excitation Working Frequency Capacitively Coupled Contactless Conductivity Detection (C4D) Sensor for Microfluidic Devices

Huang

et al. 2021

Sensors

View full text Add to dashboard Cite

In this work, a new capacitively coupled contactless conductivity detection (C4D) sensor for microfluidic devices is developed. By introducing an LC circuit, the working frequency of the new C4D sensor can be lowered by the adjustments of the inductor and the capacitance of the LC circuit. The limits of detection (LODs) of the new C4D sensor for conductivity/ion concentration measurement can be improved. Conductivity measurement experiments with KCl solutions were carried out in microfluidic devices (500 µm × 50 µm). The experimental results indicate that the developed C4D sensor can realize the conductivity measurement with low working frequency (less than 50 kHz). The LOD of the C4D sensor for conductivity measurement is estimated to be 2.2 µS/cm. Furthermore, to show the effectiveness of the new C4D sensor for the concentration measurement of other ions (solutions), SO42− and Li+ ion concentration measurement experiments were also carried out at a working frequency of 29.70 kHz. The experimental results show that at low concentrations, the input-output characteristics of the C4D sensor for SO42− and Li+ ion concentration measurement show good linearity with the LODs estimated to be 8.2 µM and 19.0 µM, respectively.

show abstract

“…[3][4][5][6][7][8] In addition, with the downsizing of ion chromatographs, mini-columns or capillary columns are employed to IC. [9][10][11][12][13][14][15] Considering the above background, we came up with use of a guard column in IC. Guard columns packed with the same ionexchange resin as the IC separation column are generally used to remove soluble impurities and extend the lifespan of the resin in the separation column.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Anion-exchange Guard Column as an Ion Chromatographic Column of Anions Including Application to Simultaneous Separation of Anions and Cations

et al. 2019

View full text Add to dashboard Cite

This study demonstrated that a guard column containing anion-exchange resin has the potential for use as a separation column for acid eluent. Specifically, a 1-cm long anion-exchange guard column with a 4.6-mm internal diameter provided good separation of monovalent inorganic anions, by elution of 8 mM tartaric acid or 4 mM malic acid. Using the guard column with acid eluent could be applied to evaluation of nitrite and nitrate ions in mountain and urban river water samples. When the guard column was connected in front of a cation-exchange separation column (15 cm long × 4.6 mm internal diameter) in a series, the system provided simultaneous separation of anions and cations in eluent of 8 mM tartaric acid and 0.5 mM 18-crown-6 ether by a single injection.

show abstract

Microfluidic membrane suppressor module design and evaluation for capillary ion chromatography

Cited by 9 publications

References 24 publications

Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins

Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins

A Low Excitation Working Frequency Capacitively Coupled Contactless Conductivity Detection (C4D) Sensor for Microfluidic Devices

Utilization of Anion-exchange Guard Column as an Ion Chromatographic Column of Anions Including Application to Simultaneous Separation of Anions and Cations

Contact Info

Product

Resources

About