Microfluidic lab-on-a-chip systems based on polymers—fabrication and application

Guber, A.E.; Heckele, M.; Herrmann, Dominik; Muslija, A.; Saile, V.; Eichhorn, L.; Gietzelt, Thomas; Hoffmann, W.; Hauser, Peter C.; Tanyanyiwa, Jatisai; Gerlach, Andreas; Gottschlich, N.; Knebel, G.

doi:10.1016/j.cej.2004.01.016

Cited by 95 publications

(59 citation statements)

References 11 publications

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“…Hence, micro-total analysis systems ( TAS) are usually designed for a well-defined analytical task [4]. A wide range of detection techniques has been employed in microfluidic devices, such as optical detection, laser induced fluorescence, luminescence, electrochemistry, surface plasmon resonance, and mass-spectroscopy [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Polymer microchip impedance spectroscopy through two parallel planar embedded microelectrodes: Understanding the impedance contribution of the surrounding polymer on the measurement accuracy

Kechadi

Gamby

Chaal

et al. 2013

Electrochimica Acta

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a b s t r a c tThe present work describes a new methodology for contact free impedance of a solution in a polymer microchip taking into account the role played by the surrounding polymer on the impedance accuracy. Measurements were carried out using a photoablated polyethylene terephthalate (PET) microchannel above two embedded microband electrodes. The impedance diagrams exhibit a loop from high frequencies to medium frequencies (1 MHz-100 Hz) and a capacitive behavior at low frequencies (100-1 Hz). The impedance diagrams were corrected by eliminating from the global microchip response the contribution of the impedance of the PET layer between the two microband electrodes. This operation enables a clear observation of the impedance in the microchannel solution, including the bulk solution contribution and the interfacial capacitance related to the surface roughness of the photoablated microchannel. Models for the impedance of solutions of varying conductivity showed that the capacitance of the polymer-solution interface can be modeled by a constant phase element (CPE) with an exponent of 0.5. The loop diameter was found to be proportional to the microchannel resistivity, allowing a cell constant around 4.93 × 10 5 m −1 in contactless microelectrodes configuration.

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Section: Introductionmentioning

confidence: 99%

Polymer microchip impedance spectroscopy through two parallel planar embedded microelectrodes: Understanding the impedance contribution of the surrounding polymer on the measurement accuracy

Kechadi

Gamby

Chaal

et al. 2013

Electrochimica Acta

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“…The use of polymers in the microfabrication of MS instruments is mainly limited in the development of miniaturized ESI sources, and these are discussed in detail in Section IIB. The use of polymers in microfluidic applications in general has been discussed in the two reviews by Becker and Gärtner (2008) and Guber et al (2004).…”

Section: Polymersmentioning

confidence: 99%

Microchip technology in mass spectrometry

Sikanen

Franssila

Kauppila

et al. 2009

Mass Spectrom. Rev.

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Microfabrication of analytical devices is currently of growing interest and many microfabricated instruments have also entered the field of mass spectrometry (MS). Various (atmospheric pressure) ion sources as well as mass analyzers have been developed exploiting microfabrication techniques. The most common approach thus far has been the miniaturization of the electrospray ion source and its integration with various separation and sampling units. Other ionization techniques, mainly atmospheric pressure chemical ionization and photoionization, have also been subject to miniaturization, though they have not attracted as much attention. Likewise, all common types of mass analyzers have been realized by microfabrication and, in most cases, successfully applied to MS analysis in conjunction with on-chip ionization. This review summarizes the latest achievements in the field of microfabricated ion sources and mass analyzers. Representative applications are reviewed focusing on the development of fully microfabricated systems where ion sources or analyzers are integrated with microfluidic separation devices or microfabricated pums and detectors, respectively. Also the main microfabrication methods, with their possibilities and constraints, are briefly discussed together with the most commonly used materials.

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“…It consists, mostly, of two perpendicular channels connecting four reservoirs. The shorter channel is generally dedicated to sample injection, whereas the longer one represents the separation channel [15] (Fig. 1).…”

Section: Microfluidic Chips For Electrophoretic Separation Of Proteinsmentioning

confidence: 99%

Recent innovations in protein separation on microchips by electrophoretic methods

Peng¹,

Pallandre²,

Tran³

et al. 2007

Electrophoresis

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Microchips for analytical purposes have attracted great attention over the last 20 years. In the present review, we focus on the most recent development of microchips for electrophoretic separation of proteins. This review starts with a short recalling about the microchips covering the basic microchip layout for CE and the commercial chips and microchip platforms. A short paragraph is dedicated to the surface treatment of microchips, which is of paramount importance in protein analysis. One section is dedicated to on-line sample pretreatment in microchips and summarizes different strategies to pre-concentrate or to purify proteins from complex matrixes. Most of the common modes used for CE of proteins have already been adapted to the chip format, while multidimensional approaches are still in progress. The different routes to achieve detection in microchip are also presented with a special attention to derivatization or labeling of proteins. Finally, several recent applications are mentioned. They highlight the great potential of electrophoretic separations of proteins in numerous fields such as biological, pharmaceutical or agricultural and food analysis. A bibliography with 151 references is provided covering papers published from 2000 to the early 2007.

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Microfluidic lab-on-a-chip systems based on polymers—fabrication and application

Cited by 95 publications

References 11 publications

Polymer microchip impedance spectroscopy through two parallel planar embedded microelectrodes: Understanding the impedance contribution of the surrounding polymer on the measurement accuracy

Polymer microchip impedance spectroscopy through two parallel planar embedded microelectrodes: Understanding the impedance contribution of the surrounding polymer on the measurement accuracy

Microchip technology in mass spectrometry

Recent innovations in protein separation on microchips by electrophoretic methods

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