Microfluidic immunosensor systems

Bange, Adam; Halsall, H. Brian; Heineman, William R.

doi:10.1016/j.bios.2004.10.016

Cited by 496 publications

(350 citation statements)

References 67 publications

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“…These steps can involve separation, mixing, incubation, washing steps and enzyme reactions with substrates necessary for the immunoassay [133]. It may also ease the automation of reactions alleviating the human error involved in the loss of accuracy and lowering the energy requirements of the assay [134].…”

Section: Miniaturisation Of Immunosensorsmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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Section: Miniaturisation Of Immunosensorsmentioning

confidence: 99%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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“…Both homogeneous and heterogeneous assays have been adapted to the microchip format [5][6][7]. A heterogeneous immunoassay exploits the interaction between a target analyte (antigen (Ag)) and a receptor (capture antibody (Ab)) immobilized on a solid phase to produce a visual, fluorescent or electrical signal, quantifying the target concentration [8]. While a continuous flow microfluidics-based bioassay allows studying the Ag-Ab binding kinetics, the intrinsically fast Ag-Ab reaction rate can be mass transport-limited [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Study of Spatio-Temporal Immunofluorescence on Bead Patterns in a Microfluidic Channel

Sivagnanam¹,

Yang

Gijs³

2010

AIP Conference Proceedings

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Abstract. We performed a direct immunoassay inside a microfluidic channel on patterned streptavidin-coated beads, which captured fluorescently-labeled biotin target molecules from a continuous flow. We arranged the beads in a dot array at the bottom of the channel and demonstrated their position-and flow rate-dependent fluorescence. As the target analyte gets gradually depleted from the flow when passing downstream the channel, the highest fluorescence intensity was observed on the most upstream positioned dot patterns. We propose a simple analytical convection model to explain this spatio-temporal fluorescence.

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“…Optical [2][3][4], piezoelectric [5], and electrochemical [6,7] immunosensors in connection with microfluidic systems [8] have successfully been applied to monitor antigen-antibody binding reactions. In recent years, immunosensors based on surface plasmon resonance (SPR) were developed for the measurement of antigens, which could bind to antibodies immobilized on the biosensor surface [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A localized surface plasmon resonance based immunosensor for the detection of casein in milk

Hiep

Endo

Kerman

et al. 2007

Science and Technology of Advanced Materials

138

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In this research, a localized surface plasmon resonance (LSPR) immunosensor based on gold-capped nanoparticle substrate for detecting casein, one of the most potent allergens in milk, was developed. The fabrication of the gold-capped nanoparticle substrate involved a surface-modified silica nanoparticle layer (core) on the slide glass substrate between bottom and top gold layers (shell). The absorbance peak of the gold-capped nanoparticle substrate was observed at $520 nm. In addition, the atomic force microscopy (AFM) images demonstrated that the nanoparticles formed a monolayer on the slide glass. After immobilizing anti-casein antibody on the surface, our device, casein immunosensor, could be applied easily for the detection of casein in the raw milk sample without a difficult pretreatment. Under the optimum conditions, the detection limit of the casein immunosensor was determined as 10 ng/mL. Our device brings several advantages to the existing LSPR-based biosensors with its easy fabrication, simple handling, low-cost, and high sensitivity. r

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Microfluidic immunosensor systems

Cited by 496 publications

References 67 publications

Trends and Perspectives in Immunosensors

Trends and Perspectives in Immunosensors

Study of Spatio-Temporal Immunofluorescence on Bead Patterns in a Microfluidic Channel

A localized surface plasmon resonance based immunosensor for the detection of casein in milk

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