Microfluidic hotspots in bacteria research: A review of soil and related advances

Dai, Hengyi; Zhuang, Yajuan; Stirling, Erinne; Liu, Nanlin; Ma, Bin

doi:10.1007/s42832-022-0129-3

Cited by 3 publications

(2 citation statements)

References 114 publications

(130 reference statements)

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“…The advent of microfabrication technologies, particularly microfluidics, has brought about a paradigm shift in contemporary biological research in recent years (Banik et al 2023 ; Dai et al 2023 ; Duncombe et al 2015 ). Generally, microfluidics pertains to the principles and instrumentation for the manipulation and analysis of fluids on a micrometer scale (Beebe et al 2002 ).…”

Section: Design Considerations When Using Microfluidics For Microbial...mentioning

confidence: 99%

Microfluidics for adaptation of microorganisms to stress: design and application

Zoheir,

Stolle,

Rabe

2024

Appl Microbiol Biotechnol

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Microfluidic systems have fundamentally transformed the realm of adaptive laboratory evolution (ALE) for microorganisms by offering unparalleled control over environmental conditions, thereby optimizing mutant generation and desired trait selection. This review summarizes the substantial influence of microfluidic technologies and their design paradigms on microbial adaptation, with a primary focus on leveraging spatial stressor concentration gradients to enhance microbial growth in challenging environments. Specifically, microfluidic platforms tailored for scaled-down ALE processes not only enable highly autonomous and precise setups but also incorporate novel functionalities. These capabilities encompass fostering the growth of biofilms alongside planktonic cells, refining selection gradient profiles, and simulating adaptation dynamics akin to natural habitats. The integration of these aspects enables shaping phenotypes under pressure, presenting an unprecedented avenue for developing robust, stress-resistant strains, a feat not easily attainable using conventional ALE setups. The versatility of these microfluidic systems is not limited to fundamental research but also offers promising applications in various areas of stress resistance. As microfluidic technologies continue to evolve and merge with cutting-edge methodologies, they possess the potential not only to redefine the landscape of microbial adaptation studies but also to expedite advancements in various biotechnological areas. Key points • Microfluidics enable precise microbial adaptation in controlled gradients. • Microfluidic ALE offers insights into stress resistance and distinguishes between resistance and persistence. • Integration of adaptation-influencing factors in microfluidic setups facilitates efficient generation of stress-resistant strains.

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Section: Design Considerations When Using Microfluidics For Microbial...mentioning

confidence: 99%

Microfluidics for adaptation of microorganisms to stress: design and application

Zoheir,

Stolle,

Rabe

2024

Appl Microbiol Biotechnol

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“…To this end, new technology may help probe the nexus between phototrophic organisms and MFCs. One promising direction involves microuidic lab-on-a-chip systems, which enable growth and real-time monitoring of living organisms such as soil bacteria, [184][185][186][187] biolms, 188,189 and even plant roots under highly controlled conditions. [190][191][192] For example, there are an increasing number of studies using microuidics to study naturally uorescent photosynthetic bacteria, sometimes…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Phototrophic microbial fuel cells: a greener approach to sustainable power generation and wastewater treatment

Sonawane¹,

Vijay²,

Deng³

et al. 2023

Sustainable Energy Fuels

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Dynamic in situ detection in iRhizo-Chip reveals diurnal fluctuations of Bacillus subtilis in the rhizosphere

Dai,

Wu,

Zhuang

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Effective colonization by microbe in the rhizosphere is critical for establishing a beneficial symbiotic relationship with the host plant. Bacillus subtilis , a soil-dwelling bacterium that is commonly found in association with plants and their rhizosphere, has garnered interest for its potential to enhance plant growth, suppress pathogens, and contribute to sustainable agricultural practices. However, research on the dynamic distribution of B. subtilis within the rhizosphere and its interaction mechanisms with plant roots remains insufficient due to limitations in existing in situ detection methodologies. To achieve dynamic in situ detection of the rhizosphere environment, we established iRhizo-Chip, a microfluidics-based platform. Using this device to investigate microbial behavior within the rhizosphere, we found obvious diurnal fluctuations in the growth of B. subtilis in the rhizosphere. Temporal dynamic analysis of rhizosphere dissolved oxygen (DO), pH, dissolved organic carbon, and reactive oxygen species showed that diurnal fluctuations in the growth of B. subtilis are potentially related to a variety of environmental factors. Spatial dynamic analysis also showed that the spatial distribution changes of B. subtilis and DO and pH were similar. Subsequently, through in vitro control experiments, we proved that rhizosphere DO and pH are the main driving forces for diurnal fluctuations in the growth of B. subtilis . Our results show that the growth of B. subtilis is driven by rhizosphere DO and pH, resulting in diurnal fluctuations, and iRhizo-Chip is a valuable tool for studying plant rhizosphere dynamics.

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Microfluidic hotspots in bacteria research: A review of soil and related advances

Cited by 3 publications

References 114 publications

Microfluidics for adaptation of microorganisms to stress: design and application

Microfluidics for adaptation of microorganisms to stress: design and application

Phototrophic microbial fuel cells: a greener approach to sustainable power generation and wastewater treatment

Dynamic in situ detection in iRhizo-Chip reveals diurnal fluctuations of Bacillus subtilis in the rhizosphere

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