Microfluidic high viability neural cell separation using viscoelastically tuned hydrodynamic spreading

Wu, Zhigang; Hjort, Klas; Wicher, Grzegorz; Svenningsen, Åsa Fex

doi:10.1007/s10544-008-9174-7

Cited by 31 publications

(24 citation statements)

References 35 publications

(45 reference statements)

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“…The throughput of our inertial microchip is 1.188 Â 10 7 cells/h, which is a significant improvement compared to using viscoelasticity tuned hydrodynamic spreading. 17 Although the throughput of the inertial chip is slower than the FACS, the throughput capability of our proposed device can be dramatically amplified by parallelizing several microchannels in the same microchip. 19 Compared to FACS, our inertial device is label-free, which may significantly reduce the total processing time and costs.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, these devices normally run in a batch manner and with a very limited throughput. Apart from DEP, Wu et al 17 also reported on the application of viscoelasticity tuned hydrodynamic spreading on neural cell separation where the microfluidic device works in a continuous and label free manner. Their device could achieve high viability neural cell separation independent of medium dielectric properties.…”

Section: B)mentioning

confidence: 99%

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A label-free and high-throughput separation of neuron and glial cells using an inertial microfluidic platform

et al. 2016

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While neurons and glial cells both play significant roles in the development and therapy of schizophrenia, their specific contributions are difficult to differentiate because the methods used to separate neurons and glial cells are ineffective and inefficient. In this study, we reported a high-throughput microfluidic platform based on the inertial microfluidic technique to rapidly and continuously separate neurons and glial cells from dissected brain tissues. The optimal working condition for an inertial biochip was investigated and evaluated by measuring its separation under different flow rates. Purified and enriched neurons in a primary neuron culture were verified by confocal immunofluorescence imaging, and neurons performed neurite growth after separation, indicating the feasibility and biocompatibility of an inertial separation. Phencyclidine disturbed the neuroplasticity and neuron metabolism in the separated and the unseparated neurons, with no significant difference. Apart from isolating the neurons, purified and enriched viable glial cells were collected simultaneously. This work demonstrates that an inertial microchip can provide a label-free, high throughput, and harmless tool to separate neurological primary cells. Published by AIP Publishing. [http://dx

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Section: Discussionmentioning

confidence: 99%

Section: B)mentioning

confidence: 99%

A label-free and high-throughput separation of neuron and glial cells using an inertial microfluidic platform

et al. 2016

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“…To simplify the tuning of cell separation a control channel, placed between the inlets and collectors and controlled with an extra pump, was designed to adapt and adjust to variations due to fabrication. By adding or subtracting fluid from the major flow, the spreading inside the major channel was shifted and finally the particle delivery could be tuned to its desired collectors 24 . …”

Section: Device Designmentioning

confidence: 99%

“…The device was then kept in a closed container with a high humidity environment in a refrigerator at 4ºC overnight. Before cell separation, the device was flushed with sterile PBS buffer 24 . For the experiments, three gastight syringes (Hamilton, Bonaduz, Switzerland) were filled with the fluids (sample and sheaths) and placed on an infusion syringe pump (PHD2000, Harvard apparatus, Boston, MA, USA).…”

Section: Device Fabrication and Fluidic Controlmentioning

confidence: 99%

Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

et al. 2009

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We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behaviour was studied in detail using 9.9 and 1.0 µ m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm 2 . The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re p ), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10 8 /mL), using a sample flow rate of up to 18 µL/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable.Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.3

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“…They have rheological properties valuable for thickening and stabilizing, which make them popular in research and in the food, paper, textiles, cosmetics and pharmaceutical industries (Vauchel et al 2008). Their rheological properties have been utilized in microfluidic devices involving ex-vivo mammalian cell preparations (Wu et al 2008). Also, alginate solutions have been introduced directly into the blood circulation of animals in some studies.…”

Section: Introductionmentioning

confidence: 99%

Rheologically biomimetic cell suspensions for decreased cell settling in microfluidic devices

Launiere

Czaplewski

Myung

et al. 2011

Biomed Microdevices

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Many microfluidic devices operate with cells suspended in buffer solutions. Researchers who work with large cell types in such devices often run into problems with gravitational cell settling in the injection equipment and in the device itself. A method for reducing this problematic settling is discussed in this paper using tumor cell lines as an example. Microfluidic circulating tumor cell (CTC) isolation devices (MCIDs) are benchmarked using buffer solutions spiked with in-vitro tumor cell lines prior to validation with clinical samples (i.e. whole blood). However, buffer solutions have different rheological properties than whole blood. Here we describe the use of alginate in PBS buffer solutions to mimic blood rheology and reduce cell settling during preliminary validation experiments. Because alginate increases the viscosity of a solution, it helps to maintain cells in suspension. We report that vertical equipment configurations are important to further mitigate the effects of cell settling for MDA-MB-468 carcinoma cells. We also report that alginate does not disrupt the specific binding interactions that are the basis of carcinoma cell capture in MCIDs. These results indicate that vertical equipment configurations and the addition of alginates can be used to reduce cell settling in buffer based MCID testing and other applications involving large cells suspended in buffer solution.

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Microfluidic high viability neural cell separation using viscoelastically tuned hydrodynamic spreading

Cited by 31 publications

References 35 publications

A label-free and high-throughput separation of neuron and glial cells using an inertial microfluidic platform

A label-free and high-throughput separation of neuron and glial cells using an inertial microfluidic platform

Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

Rheologically biomimetic cell suspensions for decreased cell settling in microfluidic devices

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