Microfluidic free‐flow zone electrophoresis and isotachophoresis using carbon black nano‐composite PDMS sidewall membranes

Fu, Xiaotong; Mavrogiannis, Nicholas; Ibo, Markela; Crivellari, Francesca; Gagnon, Zachary

doi:10.1002/elps.201600104

Cited by 24 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing with microfluidic capillary zone electrophoresis, unfortunately, continuous µFFE inevitably increases the complexity of device fabrication, induces additional stream broadening, and impairs separation performance [8]. Currently, the research field of µFFE focuses on theoretical prediction [9,10], device fabrication [11,12], electrode material selection [13,14], separation mode discussion [15,16], and application expansion [17][18][19][20]. All these efforts will promote the development of µFFE technique and the application of microfluidics in complicated real sample analysis.…”

Section: Introductionmentioning

confidence: 99%

A microchip device to enhance free flow electrophoresis using controllable pinched sample injections

et al. 2019

View full text Add to dashboard Cite

Micro free flow electrophoresis (µFFE) is a valuable technique capable of high throughput rapid microscale electrophoretic separation along with mild operating conditions. However, the stream flow separation nature of free flow electrophoresis affects its separation performance with additional stream broadening due to sample stream deflection. To reduce stream broadening and enhance separation performance of µFFE, we presented a simple microfluidic device that enables injection bandwidth control. A pinched injection was formed in the reported µFFE system using operating buffer at sample flow rate ratio (r) setting. Initial bandwidth at the entrance of separation chamber can be shrunk from 800 to 30 µm when r increased from 1 to 256. Stream broadening at the exit of separation chamber can be reduced by about 96% when r increased from 4 to 128, according to both theoretical and experimental results. Moreover, the separation resolution for a dye mixture was enhanced by a factor of 4 when r increased from 16 to 128, which corresponded to an 80% reduction in sample initial bandwidth. Furthermore, a similar enhancement on amino acids separation was obtained by using injection control in the reported µFFE device and readily integrated into online/offline sample preparation and/or downstream analysis procedures.

show abstract

Section: Introductionmentioning

confidence: 99%

A microchip device to enhance free flow electrophoresis using controllable pinched sample injections

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In some cases, FFZE has been employed as the first dimension in the multidimensional separation of complex mixtures of peptides and proteins in proteomic and peptidomic studies, e.g., in a combination with RP-HPLC-MS/MS [ 30 , 31 ] and MS [ 32 , 33 ]. Microchip formats of FFZE and other electrophoretic methods, e.g., free-flow isotachophoresis (FFITP) [ 34 ] and free-flow isoelectric focusing (FFIEF) [ 27 ] have been used for continuous microscale separation of analytes prior to their UV-absorption, fluorescence, or MS detection, for reviews see [ 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata

et al. 2021

View full text Add to dashboard Cite

The problem of a growing resistance of bacteria and other microorganisms to conventional antibiotics gave rise to a search for new potent antimicrobial agents. Insect antimicrobial peptides (AMPs) seem to be promising novel potential anti-infective therapeutics. The dipeptide β-alanyl-tyrosine (β-Ala-Tyr) is one of the endogenous insect toxins exhibiting antibacterial activity against both Gram-negative and Gram-positive bacteria. Prior to testing its other antimicrobial activities, it has to be prepared in a pure form. In this study, we have developed a capillary zone electrophoresis (CZE) method for analysis of β‑Ala‑Tyr isolated from the extract of the hemolymph of larvae of the fleshfly Neobellieria bullata by reversed-phase high-performance liquid chromatography (RP-HPLC). Based on our previously described correlation between CZE and free-flow zone electrophoresis (FFZE), analytical CZE separation of β‑Ala‑Tyr and its admixtures have been converted into preparative purification of β‑Ala‑Tyr by FFZE with preparative capacity of 45.5 mg per hour. The high purity degree of the β‑Ala‑Tyr obtained by FFZE fractionation was confirmed by its subsequent CZE analysis.

show abstract

“…Consequently, research in this field has increasingly focused on the prevention of fluidic and electrical inhomogeneity due to bubble formation. To prevent bubbles from entering the main chamber, strategies such as deepening electrode channels for an highly increased volume flow rate [11,12,26], (high resistance) side channels [27,28] between separation channel and electrode channel, and gels [22,23,29,30] and membranes [7,17,31] have been developed. Further techniques include mediation of the electric field through an insulating barrier [32], and chemical suppression of electrolysis [33].…”

Section: Introductionmentioning

confidence: 99%

Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology

et al. 2020

View full text Add to dashboard Cite

The increasing resolution of three‐dimensional (3D) printing offers simplified access to, and development of, microfluidic devices with complex 3D structures. Therefore, this technology is increasingly used for rapid prototyping in laboratories and industry. Microfluidic free flow electrophoresis (μFFE) is a versatile tool to separate and concentrate different samples (such as DNA, proteins, and cells) to different outlets in a time range measured in mere tens of seconds and offers great potential for use in downstream processing, for example. However, the production of μFFE devices is usually rather elaborate. Many designs are based on chemical pretreatment or manual alignment for the setup. Especially for the separation chamber of a μFFE device, this is a crucial step which should be automatized. We have developed a smart 3D design of a μFFE to pave the way for a simpler production. This study presents (1) a robust and reproducible way to build up critical parts of a μFFE device based on high‐resolution MultiJet 3D printing; (2) a simplified insertion of commercial polycarbonate membranes to segregate separation and electrode chambers; and (3) integrated, 3D‐printed wells that enable a defined sample fractionation (chip‐to‐world interface). In proof of concept experiments both a mixture of fluorescence dyes and a mixture of amino acids were successfully separated in our 3D‐printed μFFE device.

show abstract

Microfluidic free‐flow zone electrophoresis and isotachophoresis using carbon black nano‐composite PDMS sidewall membranes

Cited by 24 publications

References 33 publications

A microchip device to enhance free flow electrophoresis using controllable pinched sample injections

A microchip device to enhance free flow electrophoresis using controllable pinched sample injections

Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata

Miniaturized free‐flow electrophoresis: production, optimization, and application using 3D printing technology

Contact Info

Product

Resources

About