Microfluidic flow cytometry for blood-based biomarker analysis

Zhang, Yuxin; Zhao, Ying; Cole, Tim; Zheng, Jiahao; Bayinqiaoge,; Guo, Jinhong; Tang, Shi‐Yang

doi:10.1039/d2an00283c

Cited by 16 publications

(16 citation statements)

References 218 publications

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“…151,152 MFCMs still require dilution and/or a sample preparation process for the analysis of whole blood; hence, a more detailed discussion of these has not been included in this review. 150,153,154 Perhaps the sample preparation technologies discussed here could be integrated to further develop MFCMs to separate blood cells from plasma/serum and potentially analyze both components simultaneously.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…Some developments in microfluidic devices involve the isolation and analysis of blood cells and/or extracellular vesicles, which can aid in applications for liquid biopsies and cytopathology. − Flow cytometry capabilities, such as single-cell characterization and sorting, have been implemented into microfluidic devices to make portable instruments targeted at POC applications. − Recent advancements toward developing microfluidic flow cytometers (MFCMs) were summarized by Zhang et al and concluded that MFCMs do not offer a significant advantage over traditional flow cytometry, since the current detection and sorting subsystems need to be further developed to meet the cost, portability, and performance required for use in POC settings . The fabrication processes for MFCMs have been discussed in other reviews, with the general outcomes being that they are too complex and expensive for large-scale manufacturing.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…150−152 Recent advancements toward developing microfluidic flow cytometers (MFCMs) were summarized by Zhang et al and concluded that MFCMs do not offer a significant advantage over traditional flow cytometry, since the current detection and sorting subsystems need to be further developed to meet the cost, portability, and performance required for use in POC settings. 150 The fabrication processes for MFCMs have been discussed in other reviews, with the general outcomes being that they are too complex and expensive for large-scale manufacturing. Methods like soft lithography and injection molding are good alternatives with high efficiency and affordability.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…The fabrication processes for MFCMs have been discussed in other reviews, with the general outcomes being that they are too complex and expensive for large-scale manufacturing. Methods like soft lithography and injection molding are good alternatives with high efficiency and affordability. , MFCMs still require dilution and/or a sample preparation process for the analysis of whole blood; hence, a more detailed discussion of these has not been included in this review. ,, Perhaps the sample preparation technologies discussed here could be integrated to further develop MFCMs to separate blood cells from plasma/serum and potentially analyze both components simultaneously.…”

Section: Future Perspectivesmentioning

confidence: 99%

See 3 more Smart Citations

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

et al. 2023

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Blood testing allows for diagnosis and monitoring of numerous conditions and illnesses; it forms an essential pillar of the health industry that continues to grow in market value. Due to the complex physical and biological nature of blood, samples must be carefully collected and prepared to obtain accurate and reliable analysis results with minimal background signal. Examples of common sample preparation steps include dilutions, plasma separation, cell lysis, and nucleic acid extraction and isolation, which are time-consuming and can introduce risks of sample cross-contamination or pathogen exposure to laboratory staff. Moreover, the reagents and equipment needed can be costly and difficult to obtain in point-of-care or resource-limited settings. Microfluidic devices can perform sample preparation steps in a simpler, faster, and more affordable manner. Devices can be carried to areas that are difficult to access or that do not have the resources necessary. Although many microfluidic devices have been developed in the last 5 years, few were designed for the use of undiluted whole blood as a starting point, which eliminates the need for blood dilution and minimizes blood sample preparation. This review will first provide a short summary on blood properties and blood samples typically used for analysis, before delving into innovative advances in microfluidic devices over the last 5 years that address the hurdles of blood sample preparation. The devices will be categorized by application and the type of blood sample used. The final section focuses on devices for the detection of intracellular nucleic acids, because these require more extensive sample preparation steps, and the challenges involved in adapting this technology and potential improvements are discussed.

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Section: Future Perspectivesmentioning

confidence: 99%

Section: Future Perspectivesmentioning

confidence: 99%

Section: Future Perspectivesmentioning

confidence: 99%

Section: Future Perspectivesmentioning

confidence: 99%

See 2 more Smart Citations

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

et al. 2023

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“…However, conventional flow cytometry is bulky, complex, and requires highly skilled personnel. With the development of microfluidic technology, microfluidic flow cytometry (MFCM) has been combined with flow cytometry to achieve powerful single cell focusing, detection, and sorting, which has been proven in various biological applications [ 3 ], including single-cell RT-PCR [ 4 ], stem cell screening [ 5 ], protein analysis [ 6 ], etc. While MFCM has proven to be a powerful tool for single-cell manipulation and analysis in medical diagnostics and animal cell studies, similar work on plant-cell properties is still far behind.…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry

et al. 2022

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A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells. In this study, we developed a microfluidic flow cytometry with fluorescence sensor for functional characterization and phenotyping of protoplasts to allow an unbiased assessment of the influence of environmental factors at the single cell level. First, based on the measurement of intracellular homeostasis of reactive oxygen species (ROS) with a DCFH-DA dye, the effects of various external stress factors such as H2O2, temperature, ultraviolet (UV) light, and cadmium ions on intracellular ROS accumulation in Arabidopsis mesophyll protoplasts were quantitatively investigated. Second, a faster and stronger oxidative burst was observed in Petunia protoplasts isolated from white petals than in those isolated from purple petals, demonstrating the photoprotective role of anthocyanins. Third, using mutants with different endogenous auxin, we demonstrated the beneficial effect of auxin during the process of primary cell wall regeneration. Moreover, UV-B irradiation has a similar accelerating effect by increasing the intracellular auxin level, as shown by double fluorescence channels. In summary, our work has revealed previously underappreciated phenotypic variability within a protoplast population and demonstrated the advantages of a microfluidic flow cytometry for assessing the in vivo dynamics of plant metabolic and physiological indices at the single-cell level.

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Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

Li,

Xue,

et al. 2023

Electrophoresis

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Single‐cell biophysical properties play a crucial role in regulating cellular physiological states and functions, demonstrating significant potential in the fields of life sciences and clinical diagnostics. Therefore, over the last few decades, researchers have developed various detection tools to explore the relationship between the biophysical changes of biological cells and human diseases. With the rapid advancement of modern microfabrication technology, microfluidic devices have quickly emerged as a promising platform for single‐cell analysis offering advantages including high‐throughput, exceptional precision, and ease of manipulation. Consequently, this paper provides an overview of the recent advances in microfluidic analysis and detection systems for single‐cell biophysical properties and their applications in the field of cancer. The working principles and latest research progress of single‐cell biophysical property detection are first analyzed, highlighting the significance of electrical and mechanical properties. The development of data acquisition and processing methods for real‐time, high‐throughput, and practical applications are then discussed. Furthermore, the differences in biophysical properties between tumor and normal cells are outlined, illustrating the potential for utilizing single‐cell biophysical properties for tumor cell identification, classification, and drug response assessment. Lastly, we summarize the limitations of existing microfluidic analysis and detection systems in single‐cell biophysical properties, while also pointing out the prospects and future directions of their applications in cancer diagnosis and treatment.

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Microfluidic flow cytometry for blood-based biomarker analysis

Abstract: Flow cytometry has proven its capability for rapid and quantitative analysis of individual cells and the separation of targeted biological samples from others. The emerging microfluidics technology makes it possible...

Cited by 16 publications

References 218 publications

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

Emerging Microfluidic Devices for Sample Preparation of Undiluted Whole Blood to Enable the Detection of Biomarkers

Functional Characterization and Phenotyping of Protoplasts on a Microfluidics-Based Flow Cytometry

Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis

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