Microfluidic Electrospray of Multifunctional Discal Microparticles for Wound Healing

Abstract

Attempts to develop an efficient wound dressing have come to the forefront in the field of wound healing. Herein, we employ a microfluidic electrospray device to prepare the novel multifunctional discal microparticles (DMPs) with therapeutics encapsulation for wound healing. The DMPs are derived from the deformation of droplets containing κ-carrageenan and methacrylate-esterified hyaluronic acid. These DMPs possess highly porous structures due to the cross-linking and lyophilization of their hybrid hydrogels. Benefiting from the special discal morphology and porous structures, the DMPs offer candidates to wound dressing with large contact area and excellent drug release ability. By loading vascular endothelial growth factors and antimicrobial peptides, the resultant DMPs were imparted with good biocompatibility, sterilization, and angiogenesis in vitro. We have demonstrated that, in the skin defect model of Sjogren's syndrome mice, the multifunctional DMPs treated acute wounds could perform accelerated tissue regeneration and remodeling, down-regulated inflammatory response, and promoted angiogenesis in vivo. These results indicated that our proposed DMPs have great potential to serve as a satisfactory dressing for promoting wound healing.