Microfluidic droplet-based functional materials for cell manipulation

Zheng, Y.; Wu, Zengnan; Li, Gang; Zheng, Xiaonan; Hou, Ying; Lin, Jin‐Ming

doi:10.1039/d1lc00618e

Cited by 24 publications

(10 citation statements)

References 120 publications

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“…These controllable multicomponent surface droplets are of great interest in the compartmentalized chemical reactions and microanalytics. 36,76…”

Section: Formation Of Surface Nanodropletsmentioning

confidence: 99%

Nanoextraction based on surface nanodroplets for chemical preconcentration and determination

Wu¹,

Kanike²,

Atta³

et al. 2022

Preprint

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Liquid-liquid extraction based on surface nanodroplets, namely nanoextraction, can continuously extract and enrich target analytes from the flow of a sample solution. This sample preconcentration technique is easy to operate in a continuous flow system with a low consumption of organic solvent and a high enrichment factor. In this review, the evolution from single drop microextraction to advanced nanoextraction will be briefly introduced. Also, the formation principle and key features of surface nanodroplets will be summarized. Further, the major findings of nanoextraction combined with in-droplet chemistry towards sensitive and quantitative detection will be discussed. Finally, we will give our perspectives for the future trend of nanoextraction.

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“…These controllable multicomponent surface droplets are of great interest in the compartmentalized chemical reactions and microanalytics. 36,76…”

Section: Formation Of Surface Nanodropletsmentioning

confidence: 99%

Nanoextraction based on surface nanodroplets for chemical preconcentration and determination

Wu¹,

Kanike²,

Atta³

et al. 2022

Preprint

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“…Anisotropic hydrogel microparticles, which possess the precise size, shape, and surface texture, − have broken through the limitation of general isotropic particles and played a substantial role in many fields, including material science, − tissue engineering, − and sensing. − In particular, as one of the most prominent particle features, engineering surface topography has received increasing attention. − Many reports showed that actively changing the surface mesostructure of materials can generate novel biological functions, such as cell adhesion, proliferation, differentiation, and migration. − Inspired by this concept, customizing the new surface topography of hydrogel microparticles will provide opportunities for developing novel functions of materials and exploring their new applications in engineering and biology.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Engineering of Crater–Terrain Hydrogel Microparticles: Toward Novel Cell Carriers

Zheng

Hou

et al. 2023

ACS Appl. Mater. Interfaces

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Fabrication and application of novel anisotropic microparticles are of wide interest. Herein, a new method for producing novel crater−terrain hydrogel microparticles is presented using a concept of droplet−aerosol impact and regional polymerization. The surface pattern of microparticles is similar to the widespread "crater" texture on the lunar surface and can be regulated by the impact morphology of aerosols on the droplet surface. Methodological applicability was demonstrated by producing ionic-cross-linked (alginate) and photo-cross-linked (poly-(ethylene glycol) diacrylate, PEGDA) microparticles. Additionally, the crater−terrain microparticles (CTMs) can induce nonspecific protein absorption on their surface to acquire cell affinity, and they were exploited as cell carriers to load living cells. Cells could adhere and proliferate, and a special cellular adhesion fingerprint was observed on the novel cell carrier. Therefore, the scalable manufacturing method and biological potential make the engineered microparticles promising to open a new avenue for exploring cell−biomaterial crosstalk.

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“…In this review, we focus on the most recent developments in microfluidics-assisted formulation of biomaterials, in particular on those with nontrivial internal architecture, typically consisting of multiple distinct compartments. ,− ,, We use the term “microfluidics” to describe a set of techniques aimed at developing of high-level of control over tiny liquid volumes, typically nano- or even picoliter volumes, at submillimeter length scale. In particular, microfluidics can be used to disperse hydrogel precursor solutions into monodisperse droplets or extrude them into stable jets, which subsequently solidify, either spontaneously or via externally triggered cross-linking reaction, into hydrogel microparticles, ,,,,,− microfibers, − or more general “microgels”. The laminar (nonturbulent) flow conditions associated with small dimensions of the microchannels lead to reproducibility of droplet and jet morphologies as well as facilitate precise manipulation of the microscopic hydrogel liquid compartments, e.g., their on-chip merging or splitting .…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering

et al. 2022

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Microfluidics has recently emerged as a powerful tool in generation of submillimeter-sized cell aggregates capable of performing tissue-specific functions, so-called microtissues, for applications in drug testing, regenerative medicine, and cell therapies. In this work, we review the most recent advances in the field, with particular focus on the formulation of cell-encapsulating microgels of small "dimensionalities": "0D" (particles), "1D" (fibers), "2D" (sheets), etc., and with nontrivial internal topologies, typically consisting of multiple compartments loaded with different types of cells and/or biopolymers. Such structures, which we refer to as topological hydrogels or topological microgels (examples including core− shell or Janus microbeads and microfibers, hollow or porous microstructures, or granular hydrogels) can be precisely tailored with high reproducibility and throughput by using microfluidics and used to provide controlled "initial conditions" for cell proliferation and maturation into functional tissue-like microstructures. Microfluidic methods of formulation of topological biomaterials have enabled significant progress in engineering of miniature tissues and organs, such as pancreas, liver, muscle, bone, heart, neural tissue, or vasculature, as well as in fabrication of tailored microenvironments for stem-cell expansion and differentiation, or in cancer modeling, including generation of vascularized tumors for personalized drug testing. We review the available microfluidic fabrication methods by exploiting various cross-linking mechanisms and various routes toward compartmentalization and critically discuss the available tissue-specific applications. Finally, we list the remaining challenges such as simplification of the microfluidic workflow for its widespread use in biomedical research, bench-to-bedside transition including production upscaling, further in vivo validation, generation of more precise organ-like models, as well as incorporation of induced pluripotent stem cells as a step toward clinical applications.

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Microfluidic droplet-based functional materials for cell manipulation

Abstract: Functional materials from the microfluidic-based droplet community are emerging as enabling tools for various applications in tissue engineering and cell biology. The innovative micro- and nano-scale materials with diverse size,...

Cited by 24 publications

References 120 publications

Nanoextraction based on surface nanodroplets for chemical preconcentration and determination

Nanoextraction based on surface nanodroplets for chemical preconcentration and determination

Microfluidic Engineering of Crater–Terrain Hydrogel Microparticles: Toward Novel Cell Carriers

Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering

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