Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies

Sia, Samuel K.; Whitesides, George M.

doi:10.1002/elps.200305584

Cited by 1,572 publications

(1,181 citation statements)

References 82 publications

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“…Devices were fabricated from polydimethylsiloxane (PDMS) using standard lithography techniques (33,34). Molds were prepared by spin-coating 50 mm of SU-8 50 photoresist (Microchem, Newton, MA) onto 4-inch silicon wafers and then exposing to UV light through a chrome mask.…”

Section: Microfluidic Device Fabricationmentioning

confidence: 99%

Worms under Pressure: Bulk Mechanical Properties of C. elegans Are Independent of the Cuticle

Gilpin

Uppaluri

Brangwynne

2015

Biophysical Journal

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The mechanical properties of cells and tissues play a well-known role in physiology and disease. The model organism Caenorhabditis elegans exhibits mechanical properties that are still poorly understood, but are thought to be dominated by its collagen-rich outer cuticle. To our knowledge, we use a novel microfluidic technique to reveal that the worm responds linearly to low applied hydrostatic stress, exhibiting a volumetric compression with a bulk modulus, k ¼ 140 5 20 kPa; applying negative pressures leads to volumetric expansion of the worm, with a similar bulk modulus. Surprisingly, however, we find that a variety of collagen mutants and pharmacological perturbations targeting the cuticle do not impact the bulk modulus. Moreover, the worm exhibits dramatic stiffening at higher stresses-behavior that is also independent of the cuticle. The stress-strain curves for all conditions can be scaled onto a master equation, suggesting that C. elegans exhibits a universal elastic response dominated by the mechanics of pressurized internal organs.

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Section: Microfluidic Device Fabricationmentioning

confidence: 99%

Worms under Pressure: Bulk Mechanical Properties of C. elegans Are Independent of the Cuticle

Gilpin

Uppaluri

Brangwynne

2015

Biophysical Journal

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“…Lab-on-a-Chip) is a key enabling technology for point-of-care diagnostics as it promises improvements over standard immunoassay formats in terms of speed, sensitivity and reagent consumption, and can potentially be highly automated [11][12][13][14][15][16][17][18][19][20] . A low-cost disposable microfluidic card could, in principle, carry out the necessary tests using a small patient sample and a scant amount of expensive reagents, (such as antibodies).…”

Section: Microfluidics (And Its Integration Into What Is Known Asmentioning

confidence: 99%

Concentration Gradient Immunoassay. 1. An Immunoassay Based on Interdiffusion and Surface Binding in a Microchannel

2007

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We describe a novel microfluidic immunoassay method based on the diffusion of a small molecule analyte into a parallel-flowing stream containing cognate antibody. This interdiffusion results in a steady-state gradient of antibody binding site occupancy transverse to convective flow. In contrast to the diffusion immunoassay (Hatch et al. Nature Biotechnology,19:461−465 (2001)), this antibody occupancy gradient is interrogated by a sensor surface coated with a functional analog of the analyte. Antibodies with at least one unoccupied binding site may specifically bind to this functionalized surface, leading to a quantifiable change in surface coverage by the antibody. SPR imaging is used to probe the spatial distribution of antibody binding to the surface and, therefore, the outcome of the assay. We show that the pattern of antibody binding to the SPR sensing surface correlates with the concentration of a model analyte (phenytoin) in the sample stream. Using an inexpensive disposable microfluidic device, we demonstrate assays for phenytoin ranging in concentration from 75 to 1000 nM in phosphate buffer. At a total volumetric flow rate of 90 nL/sec, the assays are complete within 10 minutes. Inclusion of an additional flow stream on the side of the antibody stream opposite to that of the sample enables simultaneous calibration of the assay. This assay method is suitable for rapid quantitative detection of low-molecular weight analytes for point-of-care diagnostic instrumentation.

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“…For example, keratinocytes and EGF encapsulated into a fibrin matrix stimulated epidermal regeneration in skin wound-healing 90 , and hydrogels containing bone-forming cells, bFGF and BMP2 improved bone regeneration [91][92][93] . A more refined presentation of cell-adhesion cues in the materials using nanoscale and microscale patterning techniques [94][95][96] could provide an even greater level of control and improve the therapeutic efficiency of proteins both when host cells are recruited into the target tissue and when cells are transplanted.…”

Section: Antisense Therapymentioning

confidence: 99%

Microenvironmental regulation of biomacromolecular therapies

Kong

Mooney

2007

Nat Rev Drug Discov

138

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Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies

Cited by 1,572 publications

References 82 publications

Worms under Pressure: Bulk Mechanical Properties of C. elegans Are Independent of the Cuticle

Worms under Pressure: Bulk Mechanical Properties of C. elegans Are Independent of the Cuticle

Concentration Gradient Immunoassay. 1. An Immunoassay Based on Interdiffusion and Surface Binding in a Microchannel

Microenvironmental regulation of biomacromolecular therapies

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