Microfluidic device with a carbonate‐rich hydroxyapatite micro‐coating

Abstract

A contiguous carbonate‐rich hydroxyapatite microcoating in a microfluidic device represents a substrate that has chemical and structural similarity to bone mineral. The present work describes a low‐temperature method to deposit a carbonate‐rich hydroxyapatite microcoating on a glass slide and its incorporation within the microchannels of a microfluidic device. A glass slide is covered/masked with polypropylene‐based tape and CaCO3 nanoparticles are deposited on exposed areas by convective self assembly. The precursor CaCO3 is converted to carbonate‐rich hydroxyapatite by dissolution‐recrystallization in phosphate‐buffered saline. The microcoating is aligned/incorporated within a microchannel when the underlying glass is bonded to a polydimethylsiloxane structure with the device layout. X‐ray diffraction, laser Raman microspectroscopy, and X‐ray photoelectron spectroscopy indicate that the microcoating was comprised of carbonate‐rich hydroxyapatite. Scanning electron microscopy and 3D laser confocal microscopy showed that was comprised of nanocrystalline rod‐like clusters that collectively exhibit a thickness of ∼20 µm. Monocultures/cocultures of osteoblast‐lineage (MC3T3‐E1, MG63) and preosteoclast‐lineage (RAW 264.7) cells were performed. Osteoblast‐lineage cells adhered to the microcoating and deposited an extracellular matrix of collagen fibrils and mineral accretions. Mineralization was detected in/near the inlet wells. The microcoating is analogous to bone mineral and could be applied to various layouts and mineral systems.

Functionalizing of Polydimethylsiloxane with Cell Shape Imprinting and Nano Layer Hydroxyapatite-Coating to Accelerate Osteoblast Proliferation and Differentiation

“…Crystal violet staining was used to evaluate cell attachment, proliferation, and migration [21]. The optical density (OD) of each group was analyzed to provide quanti able data for comparison of the abovementioned characteristics.…”