Microfluidic device to study arterial shear-mediated platelet-surface interactions in whole blood: reduced sample volumes and well-characterised protein surfaces

Kent, Nigel; Basabe‐Desmonts, Lourdes; Meade, Gerardene; MacCraith, Brian D.; Corcoran, Brian; Kenny, Dermot; Ricco, Antonio J.

doi:10.1007/s10544-010-9453-y

Cited by 41 publications

(45 citation statements)

References 35 publications

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“…29 The calculated values for various devices used herein can be found in Table 1. While the equation above is generally accepted and used to estimate shear stress, this equation is obtained with an assumption that the channel width is infinite (i.e., that there are no side wall effects).…”

Section: Resultsmentioning

confidence: 99%

On-Chip Evaluation of Shear Stress Effect on Cytotoxicity of Mesoporous Silica Nanoparticles

Kim

Haynes

2011

Anal. Chem.

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In this work, nanotoxicity in the bloodstream was modeled and the cytotoxicity of sub-50 nm mesoporous silica nanoparticles to human endothelial cells was investigated under microfluidic flow conditions. Compared to traditional in vitro cytotoxicity assays performed under static conditions, unmodified mesoporous silica nanoparticles show higher and shear stress-dependent toxicity to endothelial cells under flow conditions. Interestingly, even under flow conditions, highly organo-modified mesoporous silica nanoparticles show no significant toxicity to endothelial cells. This paper clearly demonstrates that shear stress is an important factor to be considered in in vitro nanotoxicology assessments and provides a simple device for pursuing this consideration.

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Section: Resultsmentioning

confidence: 99%

On-Chip Evaluation of Shear Stress Effect on Cytotoxicity of Mesoporous Silica Nanoparticles

Kim

Haynes

2011

Anal. Chem.

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“…A simple clamping mechanism held the plates together and acrylic shim stock was placed between the plates to provide a precisely defined channel height (Figure 1B). The flow within this chamber was assumed to be one-dimensional laminar parallel plate flow (Reynolds numbers between 0.1 and 0.4) [21, 22]. …”

Section: Methodsmentioning

confidence: 99%

Visualization and analysis of biomaterial-centered thrombus formation within a defined crevice under flow

Jamiolkowski

Pedersen

et al. 2016

Biomaterials

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The blood flow pathway within a device, together with the biomaterial surfaces and status of the patient’s blood, are well-recognized factors in the development of thrombotic deposition and subsequent embolization. Blood flow patterns are of particular concern for devices such as blood pumps (i.e. ventricular assist devices, VADs) where shearing forces can be high, volumes are relatively large, and the flow fields can be complex. However, few studies have examined the effect of geometric irregularities on thrombus formation on clinically relevant opaque materials under flow. The objective of this study was to quantify human platelet deposition onto Ti6Al4V alloys, as well as positive and negative control surfaces, in the region of defined crevices (~50–150 µm in width) that might be encountered in many VADs or other cardiovascular devices. To achieve this, reconstituted fresh human blood with hemoglobin-depleted red blood cells (to achieve optical clarity while maintaining relevant rheology), long working optics, and a custom designed parallel plate flow chamber were employed. The results showed that the least amount of platelet deposition occurred in the largest crevice size examined, which was counterintuitive. The greatest levels of deposition occurred in the 90 µm and 53 µm crevices at the lower wall shear rate. The results suggest that while crevices may be unavoidable in device manufacturing, the crevice size might be tailored, depending on the flow conditions, to reduce the risk of thromboembolic events. Further, these data might be used to improve the accuracy of predictive models of thrombotic deposition in cardiovascular devices to help optimize the blood flow path and reduce device thrombogenicity.

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“…While suitable for most research, the physical method of fibronectin deposition results in coatings that are too thick, nonuniform, and unstable for studies where these qualities are important. Such research includes the use of certain microfluidic devices and flow chambers (Kent et al, 2010), as well as quartz crystal microbalance (QCM) studies where cellular properties are of interest. QCM detects changes in resonance frequencies and dissipation (for quartz crystal microbalance with dissipation, QCM D) of a quartz crystal oscillated by a shear wave resonator in order to model changes in mass and viscoelastic properties of the surface.…”

Section: Introductionmentioning

confidence: 99%

Chemically grafted fibronectin for use in QCM-D cell studies

Kandel

Lee

Sobolewski

et al. 2014

Biosensors and Bioelectronics

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Traditionally, fibronectin has been used as a physisorbed surface coating (physFN) in cell culture experiments due to its critical role in cell adhesion. However, because the resulting layer is thick, unstable, and of unpredictable uniformity, this method of fibronectin deposition is unsuitable for some types of research, including quartz crystal microbalance (QCM) experiments involving cells. Here, we present a new method for chemical immobilization of fibronectin onto silicon oxide surfaces, including QCM crystals pre-coated with silicon oxide. We characterize these chemically coated fibronectin surfaces (chemFN) as well as physFN ones using surface ellipsometry (SE), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. A cell culture model demonstrates that cells on chemFN and physFN surfaces exhibit similar viability, structure, adhesion and metabolism. Finally, we perform QCM experiments using cells on both surfaces which demonstrate the superior suitability of chemFN coatings for QCM research, and provide real-time QCM-D data from cells subjected to an actin depolymerizing agent. Overall, our method of chemical immobilization of fibronectin yields great potential for furthering cellular experiments in which thin, stable and uniform coatings are desirable. As QCM research with cells has been rather limited in success thus far, we anticipate that this new technique will particularly benefit this experimental system by availing it to the much broader field of cell mechanics.

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Microfluidic device to study arterial shear-mediated platelet-surface interactions in whole blood: reduced sample volumes and well-characterised protein surfaces

Cited by 41 publications

References 35 publications

On-Chip Evaluation of Shear Stress Effect on Cytotoxicity of Mesoporous Silica Nanoparticles

On-Chip Evaluation of Shear Stress Effect on Cytotoxicity of Mesoporous Silica Nanoparticles

Visualization and analysis of biomaterial-centered thrombus formation within a defined crevice under flow

Chemically grafted fibronectin for use in QCM-D cell studies

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