Microfluidic Device for Patient-Centric Multiplexed Assays with Readout in Centralized Laboratories

Abstract

Patient-centric sampling strategies, where the patient performs self-sampling and ships the sample to a centralized laboratory for readout, are on the verge of widespread adaptation. However, the key to a successful patient-centric workflow is user-friendliness, with few noncritical user interactions, and simple, ideally biohazard-free shipment. Here, we present a capillary-driven microfluidic device designed to perform the critical biomarker capturing step of a multiplexed immunoassay at the time of sample collection. On-chip sample drying enables biohazard-free shipment and allows us to make use of advanced analytics of specialized laboratories that offer the needed analytical sensitivity, reliability, and affordability. Using C-Reactive Protein, MCP1, S100B, IGFBP1, and IL6 as model blood biomarkers, we demonstrate the multiplexing capability and applicability of the device to a patient-centric workflow. The presented quantification of a biomarker panel opens up new possibilities for e-doctor and e-health applications.

Microfluidic Immunosensing Platform Based on a Rolling Circle Amplification-Assisted DNA Dendrimer Probe for Portable and Sensitive Detection of Allergen-Specific IgE

“…1 was used as the serum filter. The edges of paper were hydrophobicity treated to prevent leakage of blood around the edges 19 by ColorQube 8580 N wax printer (Xerox, Japan) and incubated at 120 °C for 2 min. The filtrating layer was prepared by cutting a Whatman No.…”

“…Point-ofcare test (POCT) have great promise to estimate time-critical biomarkers, which are liable to degradation after sampling. 19 When biomarkers in the blood are detected, blood cells will interfere with the sensing, reducing sensitivity and specificity. To realize POCT applications, various lab-on-chip immunoassays have been proposed, such as centrifugal 20−22 or paperbased 23−27 microfluidic chips.…”

“…Microfluidic chips can integrate basic operational units onto a centimeter-scale chip, , playing an important role in the evolution toward more patient-centered medicine. Point-of-care test (POCT) have great promise to estimate time-critical biomarkers, which are liable to degradation after sampling . When biomarkers in the blood are detected, blood cells will interfere with the sensing, reducing sensitivity and specificity.…”