Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics

Foudeh, Amir M.; Didar, Tohid F.; Veres, Teodor; Tabrizian, Maryam

doi:10.1039/c2lc40630f

Cited by 413 publications

(300 citation statements)

References 148 publications

(177 reference statements)

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“…Recently, a few LOCs have been developed for pathogen detection and some involved complex operations including total sample preparation processes, amplification, and finally detection 25 . However, to address the multiple steps involved in nucleic acid extraction and to avoid possible contamination to the downstream amplification, most of the published systems tended to perform DNA purification and amplification in two separate chambers 26,27 .…”

Section: Investigation Of the Inhibitory Effect Of Magnetic Beads On mentioning

confidence: 99%

A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples

et al. 2015

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Foodborne disease is a major public health threat worldwide. Salmonellosis, an infectious disease caused by Salmonella spp., is one of the most common foodborne diseases. Isolation and identification of Salmonella by conventional bacterial culture or molecular-based methods are time consuming and usually take a few hours to days to complete. In response to the demand for rapid on line or at site detection of pathogens, in this study, we describe for the first time an eight-chamber lab-on-a-chip (LOC) system with integrated magnetic beads-based sample preparation and loop-mediated isothermal amplification (LAMP) for rapid and quantitative detection of Salmonella spp. in food samples. The whole diagnostic procedures including DNA isolation, isothermal amplification, and real-time detection were accomplished in a single chamber. Up to eight samples could be handled simultaneously and the system was capable to detect Salmonella at concentration of 50 cells per test within 40 min. The simple design, together with high level of integration, isothermal amplification, and quantitative analysis of multiple samples in short time will greatly enhance the practical applicability of the LOC system for rapid on-site screening of Salmonella for applications in food safety control, environmental surveillance, and clinical diagnostics.

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Section: Investigation Of the Inhibitory Effect Of Magnetic Beads On mentioning

confidence: 99%

A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples

et al. 2015

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“…A microfluidic device has the ability to improve the performance of assays by facilitating less consumption of reagents, rapid analysis, high reliability and sensitivity as well as integrating multiple processes in a single device [24]. Therefore, microfluidic technology has also been exploited to design and develop various types of micro-devices for the diagnosis of tuberculosis (TB), pneumonia and asthma.…”

Section: Development Of a Diagnostic Systemmentioning

confidence: 99%

“…Treatment of TB essentially requires effective detection of infectious pathogen [24]. Tsai et al [25] presented a microfluidic paper based diagnostic device that utilized salt-induced gold nanoparticle (AuNP) based colorimetric diagnosis of TB DNA sequences.…”

Section: Development Of a Diagnostic Systemmentioning

confidence: 99%

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2016

IJBSBE

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Revisiting Pulmonary Diseases using Microfluidic IntroductionThe human respiratory system consists of nose, pharynx (throat), larynx (voice box), trachea (windpipe), bronchi, and the lungs [1]. The nose, pharynx, larynx, trachea and bronchi together form the respiratory airways. The bronchus continues to divide within lung into many narrower and shorter airways known as bronchioles [2]. The branching networks of pulmonary airways are lined with a viscous liquid film secreted by airway epithelial cells [3]. Terminal bronchioles are clustered in grapelike sacs, the alveoli. Gaseous exchange between air and blood takes place in the alveoli. The lungs contain approximately 500 million alveoli, each about 200 to 300 μm in diameter. The alveolar wall consists of a single layer of type I alveolar cells. Each alveolus is surrounded by a network of pulmonary capillaries. The interstitial space between pulmonary capillaries and alveolus forms a very thin barrier which facilitates gaseous exchange. Apart from type I alveolar cells, 5 % of the alveolar surface epithelium is covered by type II alveolar cells, which secrete a phosphor lipoprotein complex known as pulmonary surfactant that facilitates lung expansion [2]. In addition to type II alveolar cells, surfactant proteins are also produced by Clara cells of peripheral airways. The pulmonary surfactant produced from these cells is absorbed into the luminal air-liquid interface of the pulmonary airways and thus reduces the surface tension to values as low as zero during expiration. This in turn prevents the airway closure [4]. Lung epithelium is a highly complex tissue where epithelial cells, sub epithelial fibroblasts and the extracellular matrix of the airway wall are intricately involved in maintaining and regulating the structure and function of the lung [5]. Lung diseasesRespiratory diseases and disorders encompass pathological conditions that mostly affect the organs and tissues that take part in gaseous exchange (Figure 1). Notably, such abnormalities affect the airways, the physiology structure of lung tissue, and the pulmonary circulation [6]. The lung diseases are broadly classified into obstructive, restrictive and infectious diseases (Table 1).Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of death in the world. The COPD burden is projected to increase globally in the coming decades because of continued exposure to COPD risk factors and aging of the population [7]. COPD, a chronic inflammatory disease, is generally an adult age problem [8] and characterized by breathing difficulty as a consequence of narrowed airways. Long term exposure of lungs to noxious particles or gases, such as cigarette smoke, is considered as the main cause of COPD. According to WHO, COPD will become third leading cause of death by 2030 [9].Similarly, asthma is also amongst the chronic inflammatory disorders characterized by active hyper responsiveness to a variety of stimuli e.g. airborne allergens. It results from a complex interaction among inflammatory cel...

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“…This has led to fluorescence-based POC devices, in particular, as being one of the most widely explored and currently utilized optical modalities for the detection of multiple analytes on a single test. Examples of fluorescence-based POC devices being explored include LOC microfluidic immunoassays and early disease and cancer detection through biomarker isolation [91,92]. In terms of the equipment and optics, in recent years, there has been a significant advancement in portable handheld fluorescence spectrometers.…”

Section: Optical Analyses For Facilitating Poc Technologiesmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics

Cited by 413 publications

References 148 publications

A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples

A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples

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Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

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