Microfluidic chip electrophoresis for biochemical analysis

Ou, Xiaowen; Chen, Peng; Xi-zhi, Huang; Li, Shunji; Liu, Bi‐Feng

doi:10.1002/jssc.201900758

Cited by 86 publications

(52 citation statements)

References 119 publications

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“…Lastly, two analytical approaches more suitable for online action, limited samples, and increased sensitivity were evaluated: CE which is widely established for rapid on-chip separations [45][46][47] , and nanoLC-MS allowing for high sensitivity measurements 48 .…”

Section: Resultsmentioning

confidence: 99%

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Skottvoll

Hansen

Harrison

et al. 2020

Preprint

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33Liver organoids (miniature, organ-like biomaterials derived from e.g. a patient´s stem 34 cells) are emerging tools for precision drug development and toxicity screening. We 35 demonstrate that electromembrane extraction (EME) is suited for collecting organoid-36 derived drug metabolites prior to mass spectrometry (MS)-based measurements. 37 EME, which is essentially electrophoresis across an oil membrane, allowed drugs 38 and drug metabolites to be separated from medium components (albumin, etc.) that 39 could interfere with subsequent measurements. Multi-well EME (100 µL solutions) 40 allowed for simple and repeatable monitoring of heroin metabolism kinetics. Organoid 41 EME extracts were compatible with ultrahigh-pressure liquid chromatography 42 (UHPLC) and capillary electrophoresis (CE), used to separate the analytes prior to 43 detection. These initial efforts show that organoids are well-matched with various 44 electrophoresis/chromatography techniques and MS measurements. 45 46 47

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Section: Resultsmentioning

confidence: 99%

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Skottvoll

Hansen

Harrison

et al. 2020

Preprint

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“…Another less-explored method within on-chip microfluidic devices, which is steadily gaining traction, is the electrophoretic method. The advantages of microfluidic electrophoresis include low-sample requirements, cost-efficiency, rapid detection, as well as convenient integration with sensing modules [123]. Some examples of types of electrophoretic methods that have been implemented in microfluidic devices include gel electrophoresis, capillary electrophoresis, and dielectrophoresis [124,125].…”

Section: How To Detect On-sitementioning

confidence: 99%

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

et al. 2020

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More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. In the developing world, this makes waterborne diseases the second highest cause of mortality. Such cases of waterborne disease are thought to be caused by poor sanitation, water infrastructure, public knowledge, and lack of suitable water monitoring systems. Conventional laboratory-based techniques are inadequate for effective on-site water quality monitoring purposes. This is due to their need for excessive equipment, operational complexity, lack of affordability, and long sample collection to data analysis times. In this review, we discuss the conventional techniques used in modern-day water quality testing. We discuss the future challenges of water quality testing in the developing world and how conventional techniques fall short of these challenges. Finally, we discuss the development of electrochemical biosensors and current research on the integration of these devices with microfluidic components to develop truly integrated, portable, simple to use and cost-effective devices for use by local environmental agencies, NGOs, and local communities in low-resource settings.

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“…Capillary electrophoresis is a separation technique that has the potential to address this limitation as it utilizes miniaturized components, including a small power supply and detectors that can fit into a small space, thereby facilitating the manufacture of an instrument no larger than the size of a lap-top computer or smaller device [ 25 , 45 , 46 ]. Instruments developed for CE use are manufactured in two formats: a conventional platform using fused-silica capillaries, and a microfluidic chip format consisting of microchannels etched or molded into a material made of glass, silicon, or a polymer such as polydimethylsiloxane [ 25 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ]. Newer microfabrication techniques in particular are advancing, and microchip production using 3D is now under development [ 52 , 53 ].…”

Section: Role Of Capillary Electrophoresis In Point-of-care Diagnomentioning

confidence: 99%

A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

Guzman¹,

Guzman

2020

Biomedicines

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Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increasingly reliable and accessible. Particularly, the identification of circulating cell-free chemical and biochemical substances, cellular and subcellular entities, and extracellular vesicles has demonstrated promising applications in understanding the physiologic and pathologic conditions of an individual. Traditionally, tissue biopsy has been the gold standard for the diagnosis of many diseases, especially cancer. More recently, liquid biopsy for biomarker detection has emerged as a non-invasive or minimally invasive and less costly method for diagnosis of both cancerous and non-cancerous diseases, while also offering information on the progression or improvement of disease. Unfortunately, the standardization of analytical methods to isolate and quantify circulating cells and extracellular vesicles, as well as their extracted biochemical constituents, is still cumbersome, time-consuming, and expensive. To address these limitations, we have developed a prototype of a portable, miniaturized instrument that uses immunoaffinity capillary electrophoresis (IACE) to isolate, concentrate, and analyze cell-free biomarkers and/or tissue or cell extracts present in biological fluids. Isolation and concentration of analytes is accomplished through binding to one or more biorecognition affinity ligands immobilized to a solid support, while separation and analysis are achieved by high-resolution capillary electrophoresis (CE) coupled to one or more detectors. When compared to other existing methods, the process of this affinity capture, enrichment, release, and separation of one or a panel of biomarkers can be carried out on-line with the advantages of being rapid, automated, and cost-effective. Additionally, it has the potential to demonstrate high analytical sensitivity, specificity, and selectivity. As the potential of liquid biopsy grows, so too does the demand for technical advances. In this review, we therefore discuss applications and limitations of liquid biopsy and hope to introduce the idea that our affinity capture-separation device could be used as a form of point-of-care (POC) diagnostic technology to isolate, concentrate, and analyze circulating cells, extracellular vesicles, and viruses.

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Microfluidic chip electrophoresis for biochemical analysis

Cited by 86 publications

References 119 publications

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings

A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

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