Microfluidic Chip Coupled with Thermal Desorption Atmospheric Pressure Ionization Mass Spectrometry

Chang, Chen‐Chi; Chen, Tsung‐Yi; Chen, Yu-Chie

doi:10.5702/massspectrometry.s0026

Cited by 2 publications

(3 citation statements)

References 26 publications

(25 reference statements)

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“…Microfluidics is presently recognized as a crucial tool in understanding life science questions, 45 including diagnosis, 46 single-cell analysis, 18 and pharmaceuticals. 47 Several interfaces and ionization techniques can be utilized, including matrix-assisted laser desorption ionization, 48 desorption electrospray ionization, 49 atmospheric pressure photoionization, 50 inductively coupled plasma, 51 and electrospray ionization. [52][53][54][55] In addition, several geometries of microfluidic devices can be connected to a mass spectrometer to manipulate samples (including processes such as cell lysis, separation, purification, and step-wise chemical and biological reactions).…”

Section: Recent Progress On Microfluidics-based Mass Spectrometrymentioning

confidence: 99%

Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Chantipmanee¹,

2022

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Exploring unknown matter in an ultrasmall volume object, such as unknown subcellular matter in a single cell, requires an analytical technique that identifies unknown matter in terms of molecular structures and properties. Mass spectrometry is considered one of the best techniques for such analyses because it can identify unknown matter according to its mass‐to‐charge ratio. However, the use of mass spectrometry to identify unknown substances in such a small world has been greatly impeded due to the lack of tools to sample complex and heterogeneous analytes with ultrasmall volumes of the picoliter order, which is the volume order of a mammalian cell. We believe that nanofluidics would be an ideal tool to resolve this critical issue owing to its ability to sample such fluid samples with ultrasmall volumes ranging from the picoliter to zeptoliter order. Thus, the integration of such nanofluidic features into mass spectrometers would open up future avenues for the potential of mass spectrometry to explore unknown subcellular matter at a nano scale. In this perspective, we first discuss the applicability of microfluidics/nanofluidics to mass spectrometry, then address critical issues toward nanofluidics‐based mass spectrometry, and finally depict a personal outlook on the future of this field to resolve challenges on global and universal scales.

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Section: Recent Progress On Microfluidics-based Mass Spectrometrymentioning

confidence: 99%

Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Chantipmanee¹,

2022

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“…Microfluidic systems generally involve aqueous samples with low flow rates. Therefore, electrospray ionization mass spectrometry (ESI‐MS) that introduce liquid samples with a similar injection flow rate to that used in the microfluidic device is a suitable detection tool for online interfacing with microfluidic devices . Coupling microfluidic chips with ESI‐MS has been studied since the late 1990s .…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, electrospray ionization mass spectrometry (ESI-MS) [7][8][9] that introduce liquid samples with a similar injection flow rate to that used in the microfluidic device is a suitable detection tool for online interfacing with microfluidic devices. [10][11][12][13][14][15][16][17] Coupling microfluidic chips with ESI-MS has been studied since the late 1990s. 10,11 External voltage can be applied to the sample reservoir to assist the electrospray formation at the edge of the microfluidic chip.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonication‐assisted spray ionization‐based micro‐reactors for online monitoring of fast chemical reactions by mass spectrometry

Chen

2018

J Mass Spectrom

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Microfluidics can be used to handle relatively small volumes of samples and to conduct reactions in microliter‐sized volumes. Electrospray ionization can couple microfluidics with mass spectrometry (MS) to monitor chemical reactions online. However, fabricating microfluidic chips is time‐consuming. We herein propose the use of a micro‐reactor that is sustained by two capillaries and an ultrasonicator. The inlets of the capillaries were individually immersed to two different sample vials that were subjected to the ultrasonicator. The tapered outlets of the two capillaries were placed cross with an angle of ~60° close to the inlet of the mass spectrometer to fuse the eluents. On the basis of capillary action and ultrasonication, the samples from the two capillaries can be continuously directed to the capillary outlets and fuse simultaneously to generate gas phase ions for MS analysis through ultrasonication‐assisted spray ionization (UASI). Any electric contact applied on the capillaries is not required. Nevertheless, UASI spray derived from the eluents can readily occur in front of the mass spectrometer. That is, a micro‐reactor was created from the fusing of the eluent containing different reactants from these two UASI capillaries, allowing reactions to be conducted in situ. The solvent in the fused droplets was evaporated quickly, and the product ions could be immediately observed by MS because of the extreme rise in the concentration of the reactants. For proof of concept, pyrazole synthesis reaction and cortisone derivatization by Girard T reagent were selected as the model reactions. The results demonstrated the feasibility of using UASI‐based micro‐reactor for online MS analysis to detect reaction intermediates and products.

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Microfluidic Chip Coupled with Thermal Desorption Atmospheric Pressure Ionization Mass Spectrometry

Cited by 2 publications

References 26 publications

Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Toward nanofluidics‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds

Ultrasonication‐assisted spray ionization‐based micro‐reactors for online monitoring of fast chemical reactions by mass spectrometry

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