Microfluidic chip-based assay for post-hematopoietic stem cell transplantation chimerism monitoring using polymorphic tandem repeat markers

Daud, Siti Sarah; Ibrahim, Kamariah; Choong, Soo Sin; Vengidasan, Lelamekala; Chong, Lee Ai; Ariffin, Hany

doi:10.1016/j.ab.2009.10.008

Cited by 7 publications

(9 citation statements)

References 21 publications

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“…Many processing tools for HSCs are introduced and reviewed in this section including purification and separation, 145,[154][155][156][157][158] signaling analysis, [159][160][161][162][163][164] microchip electrophoresis assays, and microfluidicbased, digital, reverse transcription-polymerase chain reaction ͑RT-PCR͒ assays. [165][166][167] …”

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

“…166 Chimerism is a dynamic process which signifies the proportion of cells derived from the donor in the recipient's blood, that is, a mixture of recipient and donor cells in the recipient hematopoietic system may occur after allo-HSCT. 167 It has been reported that the determination of donor chimerism is feasible by using a microfluidic system. Engraftment status was quantitatively evaluated in postallo-HSCT patients using variable numbers of tandem repeat ͑VNTR͒ markers and microelectrophoresis technology.…”

Section: Microelectrophoresis Assay and Digital Rt-pcr Assaymentioning

confidence: 99%

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Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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Microfluidic techniques have been recently developed for cell-based assays. In microfluidic systems, the objective is for these microenvironments to mimic in vivo surroundings. With advantageous characteristics such as optical transparency and the capability for automating protocols, different types of cells can be cultured, screened, and monitored in real time to systematically investigate their morphology and functions under well-controlled microenvironments in response to various stimuli. Recently, the study of stem cells using microfluidic platforms has attracted considerable interest. Even though stem cells have been studied extensively using bench-top systems, an understanding of their behavior in in vivo-like microenvironments which stimulate cell proliferation and differentiation is still lacking. In this paper, recent cell studies using microfluidic systems are first introduced. The various miniature systems for cell culture, sorting and isolation, and stimulation are then systematically reviewed. The main focus of this review is on papers published in recent years studying stem cells by using microfluidic technology. This review aims to provide experts in microfluidics an overview of various microfluidic systems for stem cell research.

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Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

Section: Microelectrophoresis Assay and Digital Rt-pcr Assaymentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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“…[1][2][3] Complete donor derived hematopoiesis is considered essential for sustained engraftment and for preventing relapse of the underlying disease. [1][2][3][4][5][6] The critical task following HSCT is the quantitative estimation of the donor-specific cells in the recipient. Chimerism monitoring has therefore become vital for identifying as well as predicting the success or failure of HSCT.…”

Section: Introductionmentioning

confidence: 99%

Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods

et al. 2013

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“…[8] Two common types of MC are known decreasing and increasing MC. MC can be decreasing or increasing depending on the ratio between donor and recipient cells .Many variables affect the evolution of chimerism as increasing MC levels in hematopoietic stem cell transplantation (HSCT) performed after hematological malignancies may indicate disease relapse, graft failure, or rejection [1][2][3][9][10][11] . On the other hand, decreasing MC, often seen after tapering of immunosuppression after transplant or after donor lymphocytes infusion (DLI), may be an early predictor of graftversus host disease (GvHD) and of its more desirable counterpart graft versus-tumor effect.…”

Section: Jmscr Vol||04||issue||07||page 11406-11416||july 2016mentioning

confidence: 99%

“…Allogeneic-hematopoietic stem cell transplantation (HSCT) has become the main treatment of a wide range of malignant and nonmalignant hematological disorders [1][2][3][4] . The main goal of post-transplantation monitoring in HSCT is to predict negative events, such as disease relapse, graft rejection and graft-versus-host disease, in order to intervene with appropriate therapy.…”

Section: Introductionmentioning

confidence: 99%