Microfluidic Blood Typing Chip with Voltammetry Measurement

Abstract

Abstract. Blood typing is a critical test in many medical procedures, especially for blood transfusion. Incompatible red blood cells (RBCs) in transfusion might result in intravascular hemolysis and even cause recipients death. The test result of blood typing can be determined by observing the agglutination of RBCs. In this study, a microfluidic blood typing chip with interdigitated electrodes is presented. ZnO nanowires were synthesized on the interdigitated electrodes so that voltammetry measurement was employed to determine the test results of blood typing. The experimental results show that the discrimination between agglutination and non-agglutination was obvious and successful determinations of RBC agglutinations in blood typing tests were achieved by means of voltammetry measurement.

Microfluidics-Based Blood Typing Devices: An In-Depth Overview

“…RBC was separated from plasma in the device by a plasma separation membrane, and CMOS image sensing identified the results . Chang et al contrived a PDMS-based device for measuring RBC agglutination using the voltammetry technique, which consisted of interdigitated electrodes on which ZnO nanowires were synthesized by the hydrothermal method …”

“…82 Chang et al contrived a PDMS-based device for measuring RBC agglutination using the voltammetry technique, which consisted of interdigitated electrodes on which ZnO nanowires were synthesized by the hydrothermal method. 83 Kline et al contrived a plug-based device for ABO D, A, and weak A blood typing using PDMS as the substrate having a three-phase flow with liquid−liquid−gas phases one after the other to prevent cross contamination. A 1:10 v/v of surfactant 1H,1H,2H,2H-perfluoro-1-octanol (PFO) and fluoroinert 3283 was used as the carrier fluid.…”