Microfluidic based biosensing for Escherichia coli detection by embedding antimicrobial peptide-labeled beads

Yoo, Jeong Ha; Woo, Deok Ha; Chang, Mi-Sook; Chun, Myung‐Suk

doi:10.1016/j.snb.2013.09.105

Cited by 39 publications

(40 citation statements)

References 27 publications

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“…Previously, our group quantified the concentration of bacteria by evaluating the angle-specific Mie scatter signal from immunoagglutinated polystyrene particles (Fronczek et al, 2013;Park et al, 2013;You et al, 2011). By using paper microfluidic chips as our mPADs along with smartphone-based optical detection, E. coli was detected from waste water samples through quantifying the extent of immunoagglutination (Park and Yoon, 2015).…”

Section: Referencementioning

confidence: 99%

“…The concentrations of antibody-conjugated particle suspension were calibrated by measuring 45°forward light scattering of particle suspension on a 2-well slide, using the device described in our previous work (You et al, 2011). These concentration-calibrated particles were loaded to the detection area (center of each channel, next to white linear marker).…”

Section: Assay Proceduresmentioning

confidence: 99%

“…However, this soft-centrifuging resulted in varying the particle concentration. We complemented this variance by measuring the 45°forward light scattering of particle suspension using a two-well slide and the scattering reader device described in You et al (2011), followed by adding the necessary amount of deionized water to the particle suspension. Supplementary Material 2 shows microscopic images of anti-E. coli antibody-particles right after completing covalent conjugation (A) and after soft centrifugation (B), clearly indicating elimination of non-specifically aggregated antibody-particles.…”

Section: Optimization Of Particle Loading To Paper Chipsmentioning

confidence: 99%

See 2 more Smart Citations

Smartphone-based, sensitive µPAD detection of urinary tract infection and gonorrhea

Cho

Park

Nahapetian

et al. 2015

Biosensors and Bioelectronics

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Section: Referencementioning

confidence: 99%

Section: Assay Proceduresmentioning

confidence: 99%

Section: Optimization Of Particle Loading To Paper Chipsmentioning

confidence: 99%

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Smartphone-based, sensitive µPAD detection of urinary tract infection and gonorrhea

Cho

Park

Nahapetian

et al. 2015

Biosensors and Bioelectronics

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“…Likewise, AST development using microfluidics devices is possible, because have the ability of quantify antibiotic effects, enhancing sensitivity and specificity [110]. In this way an interesting approach uses microbead-based microfluidic devices for to improve detection efficiency due to the increased volume of surface immobilization [134].…”

Section: Discussionmentioning

confidence: 99%

Biosensors in Antimicrobial Drug Discovery: Since Biology until Screening Platforms

2015

J Microb Biochem Technol

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Antimicrobial drug resistance is a current public health problem, which is compounded by the misuse of antibiotics in medical practice and the emergence of Multidrug-Resistant (MDR) microorganisms. Therefore it is necessary to develop new anti-infective drugs and implement new methodologies able to establish the Antimicrobial Susceptibility (AST) in field and the point-of-care. In this sense biosensors is a promising technology that can detect MDR strains and small molecules in various samples, these devices have the advantages that can be miniaturized for obtain portability, rapidity, and cost-effectiveness. The aim of this work is to present the applications of biosensors technology in antimicrobial drug discovery, since cell based biosensors and cell culture on chips, considering metabolic interactions of the microbial world and the pharmacological response to be inhibited by compounds with promising activity with the end of design antimicrobial drug screening platforms robust, automatable and reproducible

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“…[39][40][41] The obtained bacteria were washed thrice with PBS (pH 7.4) before the SERS measurement.…”

mentioning

confidence: 99%

Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria

Wang¹,

Liu³

et al. 2018

IJN

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Background Pathogenic bacteria have always been a significant threat to human health. The detection of pathogens needs to be rapid, accurate, and convenient. Methods We present a sensitive surface-enhanced Raman scattering (SERS) biosensor based on the combination of vancomycin-modified Ag-coated magnetic nanoparticles (Fe 3 O 4 @Ag-Van MNPs) and Au@Ag nanoparticles (NPs) that can effectively capture and discriminate bacterial pathogens from solution. The high-performance Fe 3 O 4 @Ag MNPs were modified with vancomycin and used as bacteria capturer for magnetic separation and enrichment. The modified MNPS were found to exhibit strong affinity with a broad range of Gram-positive and Gram-negative bacteria. After separating and rinsing bacteria, Fe 3 O 4 @Ag-Van MNPs and Au@Ag NPs were synergistically used to construct a very large number of hot spots on bacteria cells, leading to ultrasensitive SERS detection. Results The dominant merits of our dual enhanced strategy included high bacterial-capture efficiency (>65%) within a wide pH range (pH 3.0–11.0), a short assay time (<30 min), and a low detection limit (5×10 2 cells/mL). Moreover, the spiked tests show that this method is still valid in milk and blood samples. Owing to these capabilities, the combined system enabled the sensitive and specific discrimination of different pathogens in complex solution, as verified by its detection of Gram-positive bacterium Escherichia coli , Gram-positive bacterium Staphylococcus aureus , and methicillin-resistant S. aureus . Conclusion This method has great potential for field applications in food safety, environmental monitoring, and infectious disease diagnosis.

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Microfluidic based biosensing for Escherichia coli detection by embedding antimicrobial peptide-labeled beads

Cited by 39 publications

References 27 publications

Smartphone-based, sensitive µPAD detection of urinary tract infection and gonorrhea

Smartphone-based, sensitive µPAD detection of urinary tract infection and gonorrhea

Biosensors in Antimicrobial Drug Discovery: Since Biology until Screening Platforms

Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria

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