Microfluidic Affinity Sensor Based on a Molecularly Imprinted Polymer for Ultrasensitive Detection of Chlorpyrifos

Abstract

The persistent use of pesticides in the agriculture field remains a serious issue related to public health. In the present work, molecularly imprinted polymer thin films were developed using electropolymerization of pyrrole (py) onto gold microelectrodes followed by electrodeposition for the selective detection of chlorpyrifos (CPF). The molecularly imprinted polymer (MIP) was synthesized by the electrochemical deposition method, which allowed in-line transfer of MIP on gold microelectrodes without using any additional adhering agents. Various parameters such as pH, monomer ratio, scan rate, and deposition cycle were optimized for sensor fabrication. The sensor was characterized at every stage of fabrication using various spectroscopic, microscopic, and electrochemical techniques. The sensor requires only 2 μL of the analyte and its linear detection range was found to be 1 μM to 1 fM. The developed sensor’s limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.93 and 2.82 fM, respectively, with a sensitivity of 3.98 (μA/(μM)/ mm2. The sensor’s shelf life was tested for 70 days. The applicability of the sensor in detecting CPF in fruit and vegetable samples was also assessed out with recovery % between 91 and 97% (RSD < 5%). The developed sensor possesses a huge commercial potential for on-field monitoring of pesticides.

Highly Efficient Selective Extraction of Chlorpyrifos Residues from Apples by Magnetic Microporous Molecularly Imprinted Polymer Prepared by Reversible Addition–Fragmentation Chain Transfer Surface Polymerization

“…The static adsorption experiments (Figure B) showed that the adsorption of CPF by both MMMIP and NIPs increased with the increase of the initial concentration, and MMMIP showed a superior adsorption performance to NIPs. In addition, compared with the previously reports on the adsorption of CPF by MIPs, the MMMIP synthesized by this method has a higher adsorption performance on CPF, ,,− which has potential application in the enrichment and separation of trace CPF in complex matrices.…”

Application of Molecularly Imprinted Polymers in the Analysis of Waters and Wastewaters

“…This is a consequence of CPF rebinding at the cavities in the PPy matrix, which primarily occurs via hydrogen bonding between the N group of pyridine in CPF and the N−H group of PPy. Recovery from spiked cucumber and pomegranate ranged from 91 to 97% with an RSD of 5% [117].…”

An Electroanalytical Flexible Biosensor Based on Reduced Graphene Oxide-DNA Hybrids for the Early Detection of Human Papillomavirus-16

“…Electrochemical transduction has usually been in use in biosensor design because of its high degree of sensitivity, LoD and selectivity within a wide detection range [ 7 , 8 , 9 , 10 , 11 ]. Such transduction strategies are extremely suitable for integration with flexible electrodes, and as such, have proved immensely beneficial in the point-of-care scenario [ 12 ].…”