“…The method applied here is similar to the microflotation method previously reported in Hanotu et al, (2012Hanotu et al, ( , 2013Hanotu et al, ( and 2014. After culture, the medium pH was adjusted to 5 and 7 respectively followed by the addition of chitosan for cell agglomeration at varying concentrations: (0, 0.2, 0.4, 0.6, 0.8, 1, 1.2 %v/v).…”